Cryo-EM structure of FLVCR2 in the inward-facing state with choline bound
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 8vzn
Q-score: 0.583
Cater RJ, Mukherjee D, Gil-Iturbe E, Erramilli SK, Chen T, Koo K, Santander N, Reckers A, Kloss B, Gawda T, Choy BC, Zhang Z, Katewa A, Larpthaveesarp A, Huang EJ, Mooney SWJ, Clarke OB, Yee SW, Giacomini KM, Kossiakoff AA, Quick M, Arnold T, Mancia F
Nature (2024) 629 pp. 704-709 [ DOI: doi:10.1038/s41586-024-07326-y Pubmed: 38693257 ]
- Flvcr2 in the inward-facing state complexed with choline and fab flv9 (110 kDa, Complex from Mus musculus)
- Feline leukemia virus subgroup c cellular receptor 2 (60 kDa, Protein from Mus musculus)
- Fab flv9 heavy chain (25 kDa, Protein from synthetic construct)
- Fab flv9 light chain (23 kDa, Protein from synthetic construct)
- Choline ion (104 Da, Ligand)
Cryo-EM structure of FLVCR2 in the outward-facing state with choline bound
Resolution: 2.49 Å
EM Method: Single-particle
Fitted PDBs: 8vzo
Q-score: 0.637
Cater RJ, Mukherjee D, Gil-Iturbe E, Erramilli SK, Chen T, Koo K, Santander N, Reckers A, Kloss B, Gawda T, Choy BC, Zhang Z, Katewa A, Larpthaveesarp A, Huang EJ, Mooney SWJ, Clarke OB, Yee SW, Giacomini KM, Kossiakoff AA, Quick M, Arnold T, Mancia F
Nature (2024) 629 pp. 704-709 [ DOI: doi:10.1038/s41586-024-07326-y Pubmed: 38693257 ]
- Flvcr2 in the outward-facing state complexed with choline and fab flv23 (110 kDa, Complex from Mus musculus)
- Feline leukemia virus subgroup c cellular receptor 2 (60 kDa, Protein from Mus musculus)
- Fab flv23 heavy chain (25 kDa, Protein from synthetic construct)
- Fab flv23 light chain (23 kDa, Protein from synthetic construct)
- Choline ion (104 Da, Ligand)
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 8pg0
Q-score: 0.528
Ciuta AD, Nosol K, Kowal J, Mukherjee S, Ramirez AS, Stieger B, Kossiakoff AA, Locher KP
Nat Commun (2023) 14 pp. 5774-5774 [ Pubmed: 37723174 DOI: doi:10.1038/s41467-023-41552-8 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Fab19 (heavy chain, variable region) (25 kDa, Protein from Homo sapiens)
- Bicarbonate ion (61 Da, Ligand)
- Solute carrier organic anion transporter family member 1b3 (77 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Human oatp1b3 (180 kDa, Complex from Homo sapiens)
- Fab19 (light chain, variable region) (23 kDa, Protein from Homo sapiens)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8phw
Q-score: 0.38
Ciuta AD, Nosol K, Kowal J, Mukherjee S, Ramirez AS, Stieger B, Kossiakoff AA, Locher KP
Nat Commun (2023) 14 pp. 5774-5774 [ Pubmed: 37723174 DOI: doi:10.1038/s41467-023-41552-8 ]
- Fab18 (light chain, variable region) (23 kDa, Protein from Homo sapiens)
- Fab18 (heavy chain, variable region) (24 kDa, Protein from Homo sapiens)
- Solute carrier organic anion transporter family member 1b1 (Complex from Homo sapiens)
- Fab18 (variable region) (Complex from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Estrone 3-sulfate (350 Da, Ligand)
- Human oatp1b1 (180 kDa, Complex)
- Solute carrier organic anion transporter family member 1b1 (76 kDa, Protein from Homo sapiens)
Resolution: 2.72 Å
EM Method: Single-particle
Fitted PDBs: 7zlg
Q-score: 0.454
Bloch JS, John A, Mao R, Mukherjee S, Boilevin J, Irobalieva RN, Darbre T, Scott NE, Reymond JL, Kossiakoff AA, Goddard-Borger ED, Locher KP
Nat Chem Biol (2023) 19 pp. 575-584 [ Pubmed: 36604564 DOI: doi:10.1038/s41589-022-01219-9 ]
- C-mannosyltransferase dpy-19 (80 kDa, Protein from Caenorhabditis elegans)
- Cmt2-fab heavy chain (25 kDa, Protein from synthetic construct)
- Anti-fab nanobody (13 kDa, Protein from synthetic construct)
- Synthetic octapeptide wehi 1886493 (821 Da, Protein from synthetic construct)
- Cmt2-fab light chain (23 kDa, Protein from synthetic construct)
- C-mannosyltransferase cedpy19, in complex with acceptor peptide and bound to cmt2-fab and anti-fab nanobody (Complex from Caenorhabditis elegans)
- Water (18 Da, Ligand)
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 7zlh
Q-score: 0.455
Bloch JS, John A, Mao R, Mukherjee S, Boilevin J, Irobalieva RN, Darbre T, Scott NE, Reymond JL, Kossiakoff AA, Goddard-Borger ED, Locher KP
Nat Chem Biol (2023) 19 pp. 575-584 [ Pubmed: 36604564 DOI: doi:10.1038/s41589-022-01219-9 ]
- C-mannosyltransferase dpy-19 (80 kDa, Protein from Caenorhabditis elegans)
- Cmt2-fab heavy chain (25 kDa, Protein from synthetic construct)
- Anti-fab nanobody (13 kDa, Protein from synthetic construct)
- Cmt2-fab light chain (23 kDa, Protein from synthetic construct)
- Water (18 Da, Ligand)
- C-mannosyltransferase cedpy19, in apo state, bound to cmt2-fab and anti-fab nanobody (Complex from Caenorhabditis elegans)
Resolution: 2.99 Å
EM Method: Single-particle
Fitted PDBs: 7zli
Q-score: 0.496
Bloch JS, John A, Mao R, Mukherjee S, Boilevin J, Irobalieva RN, Darbre T, Scott NE, Reymond JL, Kossiakoff AA, Goddard-Borger ED, Locher KP
Nat Chem Biol (2023) 19 pp. 575-584 [ Pubmed: 36604564 DOI: doi:10.1038/s41589-022-01219-9 ]
- C-mannosyltransferase dpy-19 (80 kDa, Protein from Caenorhabditis elegans)
- Cmt2-fab heavy chain (25 kDa, Protein from synthetic construct)
- Anti-fab nanobody (13 kDa, Protein from synthetic construct)
- Cmt2-fab light chain (23 kDa, Protein from synthetic construct)
- C-mannosyltransferase cedpy19, in complex with dol25-p-man and bound to cmt2-fab and anti-fab nanobody (Complex from Caenorhabditis elegans)
- [(2~{s},3~{s},4~{s},5~{s},6~{r})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl] [(3~{s},6~{z},10~{e},14~{e})-3,7,11,15,19-pentamethylicosa-6,10,14,18-tetraenyl] hydrogen phosphate (602 Da, Ligand)
- Water (18 Da, Ligand)
Resolution: 3.63 Å
EM Method: Single-particle
Fitted PDBs: 7zlj
Q-score: 0.411
Bloch JS, John A, Mao R, Mukherjee S, Boilevin J, Irobalieva RN, Darbre T, Scott NE, Reymond JL, Kossiakoff AA, Goddard-Borger ED, Locher KP
Nat Chem Biol (2023) 19 pp. 575-584 [ Pubmed: 36604564 DOI: doi:10.1038/s41589-022-01219-9 ]
- C-mannosyltransferase dpy-19 (80 kDa, Protein from Caenorhabditis elegans)
- Cmt2-fab heavy chain (25 kDa, Protein from synthetic construct)
- Anti-fab nanobody (13 kDa, Protein from synthetic construct)
- Cmt2-fab light chain (23 kDa, Protein from synthetic construct)
- [(2~{r},3~{s},4~{s},5~{s},6~{r})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]methyl-[(3~{s},6~{e},10~{z},14~{e})-3,7,11,15,19-pentamethylicosa-6,10,14,18-tetraenoxy]phosphinic acid (600 Da, Ligand)
- Synthetic octapeptide wehi 1886493 (821 Da, Protein from synthetic construct)
- C-mannosyltransferase cedpy19, in ternary complex with dol25-p-c-man and acceptor peptide, bound to cmt2-fab and anti-fab nanobody (Complex from Caenorhabditis elegans)
- Water (18 Da, Ligand)
Cryo-EM structure of the HIV-1 restriction factor human SERINC3
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 7ru6
Q-score: 0.323
Leonhardt SA, Purdy MD, Grover JR, Yang Z, Poulos S, McIntire WE, Tatham EA, Erramilli SK, Nosol K, Lai KK, Ding S, Lu M, Uchil PD, Finzi A, Rein A, Kossiakoff AA, Mothes W, Yeager M
Nat Commun (2023) 14 pp. 4368-4368 [ Pubmed: 37474505 DOI: doi:10.1038/s41467-023-39262-2 ]
- Serine incorporator 3 (55 kDa, Protein from Homo sapiens)
- Sia (25 kDa, Protein from synthetic construct)
- Hserinc3-sia (55 kDa, Complex from Homo sapiens)
Resolution: 4.7 Å
EM Method: Single-particle
Fitted PDBs: 7rug
Q-score: 0.244
Leonhardt SA, Purdy MD, Grover JR, Yang Z, Poulos S, McIntire WE, Tatham EA, Erramilli SK, Nosol K, Lai KK, Ding S, Lu M, Uchil PD, Finzi A, Rein A, Kossiakoff AA, Mothes W, Yeager M
Nat Commun (2023) 14 pp. 4368-4368 [ Pubmed: 37474505 DOI: doi:10.1038/s41467-023-39262-2 ]
- Sia (25 kDa, Protein from synthetic construct)
- Serine incorporator 3 (52 kDa, Protein from Homo sapiens)
- Hserinc3-deltaicl4-sia (52 kDa, Complex from Homo sapiens)