Resolution: 2.72 Å
EM Method: Single-particle
Fitted PDBs: 7zlg
Q-score: 0.454
Bloch JS, John A, Mao R, Mukherjee S, Boilevin J, Irobalieva RN, Darbre T, Scott NE, Reymond JL, Kossiakoff AA, Goddard-Borger ED, Locher KP
Nat Chem Biol (2023) 19 pp. 575-584 [ Pubmed: 36604564 DOI: doi:10.1038/s41589-022-01219-9 ]
- Synthetic octapeptide wehi 1886493 (821 Da, Protein from synthetic construct)
- Cmt2-fab heavy chain (25 kDa, Protein from synthetic construct)
- Water (18 Da, Ligand)
- C-mannosyltransferase cedpy19, in complex with acceptor peptide and bound to cmt2-fab and anti-fab nanobody (Complex from Caenorhabditis elegans)
- C-mannosyltransferase dpy-19 (80 kDa, Protein from Caenorhabditis elegans)
- Anti-fab nanobody (13 kDa, Protein from synthetic construct)
- Cmt2-fab light chain (23 kDa, Protein from synthetic construct)
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 7zlh
Q-score: 0.455
Bloch JS, John A, Mao R, Mukherjee S, Boilevin J, Irobalieva RN, Darbre T, Scott NE, Reymond JL, Kossiakoff AA, Goddard-Borger ED, Locher KP
Nat Chem Biol (2023) 19 pp. 575-584 [ Pubmed: 36604564 DOI: doi:10.1038/s41589-022-01219-9 ]
- Cmt2-fab heavy chain (25 kDa, Protein from synthetic construct)
- Water (18 Da, Ligand)
- C-mannosyltransferase dpy-19 (80 kDa, Protein from Caenorhabditis elegans)
- Anti-fab nanobody (13 kDa, Protein from synthetic construct)
- C-mannosyltransferase cedpy19, in apo state, bound to cmt2-fab and anti-fab nanobody (Complex from Caenorhabditis elegans)
- Cmt2-fab light chain (23 kDa, Protein from synthetic construct)
Resolution: 2.99 Å
EM Method: Single-particle
Fitted PDBs: 7zli
Q-score: 0.496
Bloch JS, John A, Mao R, Mukherjee S, Boilevin J, Irobalieva RN, Darbre T, Scott NE, Reymond JL, Kossiakoff AA, Goddard-Borger ED, Locher KP
Nat Chem Biol (2023) 19 pp. 575-584 [ Pubmed: 36604564 DOI: doi:10.1038/s41589-022-01219-9 ]
- Cmt2-fab heavy chain (25 kDa, Protein from synthetic construct)
- Water (18 Da, Ligand)
- C-mannosyltransferase dpy-19 (80 kDa, Protein from Caenorhabditis elegans)
- Anti-fab nanobody (13 kDa, Protein from synthetic construct)
- Cmt2-fab light chain (23 kDa, Protein from synthetic construct)
- C-mannosyltransferase cedpy19, in complex with dol25-p-man and bound to cmt2-fab and anti-fab nanobody (Complex from Caenorhabditis elegans)
- [(2~{s},3~{s},4~{s},5~{s},6~{r})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl] [(3~{s},6~{z},10~{e},14~{e})-3,7,11,15,19-pentamethylicosa-6,10,14,18-tetraenyl] hydrogen phosphate (602 Da, Ligand)
Resolution: 3.63 Å
EM Method: Single-particle
Fitted PDBs: 7zlj
Q-score: 0.411
Bloch JS, John A, Mao R, Mukherjee S, Boilevin J, Irobalieva RN, Darbre T, Scott NE, Reymond JL, Kossiakoff AA, Goddard-Borger ED, Locher KP
Nat Chem Biol (2023) 19 pp. 575-584 [ Pubmed: 36604564 DOI: doi:10.1038/s41589-022-01219-9 ]
- Cmt2-fab heavy chain (25 kDa, Protein from synthetic construct)
- Water (18 Da, Ligand)
- C-mannosyltransferase dpy-19 (80 kDa, Protein from Caenorhabditis elegans)
- Anti-fab nanobody (13 kDa, Protein from synthetic construct)
- C-mannosyltransferase cedpy19, in ternary complex with dol25-p-c-man and acceptor peptide, bound to cmt2-fab and anti-fab nanobody (Complex from Caenorhabditis elegans)
- Synthetic octapeptide wehi 1886493 (821 Da, Protein from synthetic construct)
- Cmt2-fab light chain (23 kDa, Protein from synthetic construct)
- [(2~{r},3~{s},4~{s},5~{s},6~{r})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]methyl-[(3~{s},6~{e},10~{z},14~{e})-3,7,11,15,19-pentamethylicosa-6,10,14,18-tetraenoxy]phosphinic acid (600 Da, Ligand)
Cryo-EM structure of human insulin degrading enzyme in complex with insulin
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 6bf8
Q-score: 0.153
Zhang Z, Liang WG, Bailey LJ, Tan YZ, Wei H, Wang A, Farcasanu M, Woods VA, McCord LA, Lee D, Shang W, Deprez-Poulain R, Deprez B, Liu DR, Koide A, Koide S, Kossiakoff AA, Li S, Carragher B, Potter CS, Tang WJ
eLife (2018) 7 [ DOI: doi:10.7554/eLife.33572 Pubmed: 29596046 ]
- Insulin-degrading enzyme (111 kDa, Protein from Homo sapiens)
- Insulin degrading enzyme (100 kDa, Complex from Homo sapiens)
Cryo-EM structure of human insulin degrading enzyme
Resolution: 6.5 Å
EM Method: Single-particle
Fitted PDBs: 6bf6
Q-score: 0.162
Zhang Z, Liang WG, Bailey LJ, Tan YZ, Wei H, Wang A, Farcasanu M, Woods VA, McCord LA, Lee D, Shang W, Deprez-Poulain R, Deprez B, Liu DR, Koide A, Koide S, Kossiakoff AA, Li S, Carragher B, Potter CS, Tang WJ
eLife (2018) 7 [ DOI: doi:10.7554/eLife.33572 Pubmed: 29596046 ]
- Insulin-degrading enzyme (111 kDa, Protein from Homo sapiens)
- Insulin degrading enzyme (100 kDa, Complex from Homo sapiens)
Resolution: 6.5 Å
EM Method: Single-particle
Fitted PDBs: 6bf7
Q-score: 0.16
Zhang Z, Liang WG, Bailey LJ, Tan YZ, Wei H, Wang A, Farcasanu M, Woods VA, McCord LA, Lee D, Shang W, Deprez-Poulain R, Deprez B, Liu DR, Koide A, Koide S, Kossiakoff AA, Li S, Carragher B, Potter CS, Tang WJ
eLife (2018) 7 [ DOI: doi:10.7554/eLife.33572 Pubmed: 29596046 ]
- Fab h11-e light chain (23 kDa, Protein from Homo sapiens)
- Insulin-degrading enzyme (111 kDa, Protein from Homo sapiens)
- Insulin degrading enzyme (100 kDa, Complex from Homo sapiens)
- Fab h11-e heavy chain (23 kDa, Protein from Homo sapiens)
Resolution: 7.2 Å
EM Method: Single-particle
Fitted PDBs: 6bf9
Q-score: 0.156
Zhang Z, Liang WG, Bailey LJ, Tan YZ, Wei H, Wang A, Farcasanu M, Woods VA, McCord LA, Lee D, Shang W, Deprez-Poulain R, Deprez B, Liu DR, Koide A, Koide S, Kossiakoff AA, Li S, Carragher B, Potter CS, Tang WJ
eLife (2018) 7 [ DOI: doi:10.7554/eLife.33572 Pubmed: 29596046 ]
- Fab h11-e light chain (23 kDa, Protein from Homo sapiens)
- Insulin-degrading enzyme (111 kDa, Protein from Homo sapiens)
- Insulin degrading enzyme (100 kDa, Complex from Homo sapiens)
- Fab h11-e heavy chain (23 kDa, Protein from Homo sapiens)
Cryo-EM structure of human insulin degrading enzyme in complex with insulin
Resolution: 6.5 Å
EM Method: Single-particle
Fitted PDBs: 6b7z
Q-score: 0.146
Zhang Z, Liang WG, Bailey LJ, Tan YZ, Wei H, Wang A, Farcasanu M, Woods VA, McCord LA, Lee D, Shang W, Deprez-Poulain R, Deprez B, Liu DR, Koide A, Koide S, Kossiakoff AA, Li S, Carragher B, Potter CS, Tang WJ
eLife (2018) 7 [ DOI: doi:10.7554/eLife.33572 Pubmed: 29596046 ]
- Fab h11 heavy chain (23 kDa, Protein from Homo sapiens)
- Insulin-degrading enzyme (111 kDa, Protein from Homo sapiens)
- Insulin degrading enzyme (100 kDa, Complex from Homo sapiens)
- Fab h11 light chain (23 kDa, Protein from Homo sapiens)
Cryo-EM structure of human insulin degrading enzyme in complex with insulin
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6b70
Q-score: 0.374
Zhang Z, Liang WG, Bailey LJ, Tan YZ, Wei H, Wang A, Farcasanu M, Woods VA, McCord LA, Lee D, Shang W, Deprez-Poulain R, Deprez B, Liu DR, Koide A, Koide S, Kossiakoff AA, Li S, Carragher B, Potter CS, Tang WJ
eLife (2018) 7 [ DOI: doi:10.7554/eLife.33572 Pubmed: 29596046 ]
- Insulin-degrading enzyme (111 kDa, Protein from Homo sapiens)
- Insulin (11 kDa, Protein from Homo sapiens)
- Insulin degrading enzyme/insulin (100 kDa, Complex from Homo sapiens)
- Fab h11-e light chain (23 kDa, Protein from Homo sapiens)
- Fab h11-e heavy chain (23 kDa, Protein from Homo sapiens)