Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8phw
Q-score: 0.38
Ciuta AD, Nosol K, Kowal J, Mukherjee S, Ramirez AS, Stieger B, Kossiakoff AA, Locher KP
Nat Commun (2023) 14 pp. 5774-5774 [ DOI: doi:10.1038/s41467-023-41552-8 Pubmed: 37723174 ]
- Human oatp1b1 (180 kDa, Complex)
- Solute carrier organic anion transporter family member 1b1 (76 kDa, Protein from Homo sapiens)
- Fab18 (light chain, variable region) (23 kDa, Protein from Homo sapiens)
- Solute carrier organic anion transporter family member 1b1 (Complex from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Fab18 (variable region) (Complex from Homo sapiens)
- Fab18 (heavy chain, variable region) (24 kDa, Protein from Homo sapiens)
- Estrone 3-sulfate (350 Da, Ligand)
Cryo-EM structure of the HIV-1 restriction factor human SERINC3
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 7ru6
Q-score: 0.323
Leonhardt SA, Purdy MD, Grover JR, Yang Z, Poulos S, McIntire WE, Tatham EA, Erramilli SK, Nosol K, Lai KK, Ding S, Lu M, Uchil PD, Finzi A, Rein A, Kossiakoff AA, Mothes W, Yeager M
Nat Commun (2023) 14 pp. 4368-4368 [ DOI: doi:10.1038/s41467-023-39262-2 Pubmed: 37474505 ]
- Sia (25 kDa, Protein from synthetic construct)
- Hserinc3-sia (55 kDa, Complex from Homo sapiens)
- Serine incorporator 3 (55 kDa, Protein from Homo sapiens)
Resolution: 4.7 Å
EM Method: Single-particle
Fitted PDBs: 7rug
Q-score: 0.244
Leonhardt SA, Purdy MD, Grover JR, Yang Z, Poulos S, McIntire WE, Tatham EA, Erramilli SK, Nosol K, Lai KK, Ding S, Lu M, Uchil PD, Finzi A, Rein A, Kossiakoff AA, Mothes W, Yeager M
Nat Commun (2023) 14 pp. 4368-4368 [ DOI: doi:10.1038/s41467-023-39262-2 Pubmed: 37474505 ]
- Hserinc3-deltaicl4-sia (52 kDa, Complex from Homo sapiens)
- Sia (25 kDa, Protein from synthetic construct)
- Serine incorporator 3 (52 kDa, Protein from Homo sapiens)
Structure of the human sodium/bile acid cotransporter (NTCP) in complex with Fab and nanobody
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 7zyi
Q-score: 0.502
Liu H, Irobalieva RN, Bang-Sorensen R, Nosol K, Mukherjee S, Agrawal P, Stieger B, Kossiakoff AA, Locher KP
Cell Res (2022) 32 pp. 773-776 [ Pubmed: 35726088 DOI: doi:10.1038/s41422-022-00680-4 ]
- Water (18 Da, Ligand)
- Nanobody (13 kDa, Protein from Lama glama)
- Glycochenodeoxycholic acid (449 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Sodium/bile acid cotransporter (38 kDa, Protein from Homo sapiens)
- Light chain of fab (23 kDa, Protein from Mus musculus)
- Heavy chain of fab (25 kDa, Protein from Mus musculus)
- Cholesterol (386 Da, Ligand)
- Sodium/bile acid cotransporter (ntcp) in complex with fab and nanobody (110 kDa, Complex from Homo sapiens)
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 7niu
Q-score: 0.333
Nosol K, Bang-Sorensen R, Irobalieva RN, Erramilli SK, Stieger B, Kossiakoff AA, Locher KP
PNAS (2021) 118 [ Pubmed: 34385322 DOI: doi:10.1073/pnas.2106702118 ]
- 4b1 fab-fragment heavy chain (25 kDa, Protein from Homo sapiens)
- Qa2 fab-fragment light chain (23 kDa, Protein from Homo sapiens)
- 4b1 fab-fragment: light and heavy chain (Complex from Homo sapiens)
- Isoform 2 of phosphatidylcholine translocator abcb4 (140 kDa, Protein from Homo sapiens)
- Nanodisc reconstituted human abcb4 in complex with 4b1-fab and qa2-fab (apo-inward-open conformation) (340 kDa, Complex)
- Qa2 fab-fragment: light and heavy chain (Complex from Homo sapiens)
- Qa2 fab-fragment heavy chain (24 kDa, Protein from Homo sapiens)
- Phosphatidylcholine translocator abcb4 (Complex from Homo sapiens)
- 4b1 fab-fragment light chain (23 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7niv
Q-score: 0.382
Nosol K, Bang-Sorensen R, Irobalieva RN, Erramilli SK, Stieger B, Kossiakoff AA, Locher KP
PNAS (2021) 118 [ Pubmed: 34385322 DOI: doi:10.1073/pnas.2106702118 ]
- Qa2 fab-fragment light chain (23 kDa, Protein from Homo sapiens)
- Qa2 fab-fragment: light and heavy chain (Complex from Homo sapiens)
- 4b1 fab-fragment: light and heavy chain (Complex from Homo sapiens)
- Isoform 2 of phosphatidylcholine translocator abcb4 (140 kDa, Protein from Homo sapiens)
- 1,2-dilinoleoyl-sn-glycero-3-phosphocholine (782 Da, Ligand)
- Qa2 fab-fragment light chain (24 kDa, Protein from Homo sapiens)
- Phosphatidylcholine translocator abcb4 (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- 4b1 fab-fragment light chain (23 kDa, Protein from Homo sapiens)
- Nanodisc reconstituted human abcb4 in complex with 4b1-fab and qa2-fab (phosphatidylcholine-bound, occluded conformation) (340 kDa, Complex)
- 4b1 fab-fragment light chain (25 kDa, Protein from Homo sapiens)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7niw
Q-score: 0.452
Nosol K, Bang-Sorensen R, Irobalieva RN, Erramilli SK, Stieger B, Kossiakoff AA, Locher KP
PNAS (2021) 118 [ Pubmed: 34385322 DOI: doi:10.1073/pnas.2106702118 ]
- 4b1 fab-fragment heavy chain (25 kDa, Protein from Homo sapiens)
- Nanodisc reconstituted human abcb4 in complex with 4b1-fab (posaconazole-bound, inward-open conformation) (140 kDa, Complex)
- 1,2-dilinoleoyl-sn-glycero-3-phosphocholine (782 Da, Ligand)
- Phosphatidylcholine translocator abcb4 (Complex from Homo sapiens)
- 4b1 fab-fragment: heavy chain (Complex from Homo sapiens)
- 4b1 fab-fragment: light chain (Complex from Homo sapiens)
- 4b1 fab-fragment light chain (23 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Phosphatidylcholine translocator abcb4 (140 kDa, Protein from Homo sapiens)
- Posaconazole (700 Da, Ligand)