Cas9 ternary complex, 14-nt sgRNA, State I (linear)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 9c9p
Q-score: 0.461
Kiernan KA, Kwon J, Merrill BJ, Simonovic M
Nucleic Acids Res (2025) 53 [ DOI: doi:10.1093/nar/gkae1164 Pubmed: 39657754 ]
- Sgrna (30 kDa, RNA from synthetic construct)
- Cas9-sgrna-dsdna complex (213 MDa, Complex from Streptococcus pyogenes)
- Target strand (12 kDa, DNA from synthetic construct)
- Crispr-associated endonuclease cas9/csn1 (158 kDa, Protein from Streptococcus pyogenes)
- Non-target strand (12 kDa, DNA from synthetic construct)
Cas9 ternary complex, 14-nt sgRNA, State II (kinked)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 9cgu
Q-score: 0.404
Kiernan KA, Kwon J, Merrill BJ, Simonovic M
Nucleic Acids Res (2025) 53 [ DOI: doi:10.1093/nar/gkae1164 Pubmed: 39657754 ]
- Sgrna (30 kDa, RNA from synthetic construct)
- Cas9-sgrna-dsdna complex (213 kDa, Complex from Streptococcus pyogenes)
- Target strand (12 kDa, DNA from synthetic construct)
- Crispr-associated endonuclease cas9/csn1 (158 kDa, Protein from Streptococcus pyogenes)
- Non-target strand (12 kDa, DNA from synthetic construct)
Cryo-EM structure of human TMEM87A A308M
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8kb4
Q-score: 0.507
Kang H, Han AR, Zhang A, Jeong H, Koh W, Lee JM, Lee H, Jo HY, Maria-Solano MA, Bhalla M, Kwon J, Roh WS, Yang J, An HJ, Choi S, Kim HM, Lee CJ
Nat Commun (2024) 15 pp. 5830-5830 [ Pubmed: 38992057 DOI: doi:10.1038/s41467-024-49297-8 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Transmembrane protein 87a,egfp (96 kDa, Protein from Human adenovirus C serotype 2)
- (9r,12r)-15-amino-12-hydroxy-6,12-dioxo-7,11,13-trioxa-12lambda~5~-phosphapentadecan-9-yl undecanoate (481 Da, Ligand)
- Transmembrane protein 87a a308m with gfp tag and twin-strep tag (Cellular component from Homo sapiens)
Cryo-EM structure of human TMEM87A, PE-bound
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8hsi
Q-score: 0.493
Kang H, Han AR, Zhang A, Jeong H, Koh W, Lee JM, Lee H, Jo HY, Maria-Solano MA, Bhalla M, Kwon J, Roh WS, Yang J, An HJ, Choi S, Kim HM, Lee CJ
Nat Commun (2024) 15 pp. 5830-5830 [ Pubmed: 38992057 DOI: doi:10.1038/s41467-024-49297-8 ]
- Transmembrane protein 87a (63 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- (1s)-2-{[(s)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(octadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (746 Da, Ligand)
- Transmembrane protein 87a with gfp tag and twin-strep tag (Cellular component from Homo sapiens)
Cryo-EM structure of human TMEM87A, gluconate-bound
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8htt
Q-score: 0.427