E. coli DNA gyrase bound to a DNA crossover
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8qdx
Q-score: 0.451
Vayssieres M, Marechal N, Yun L, Lopez Duran B, Murugasamy NK, Fogg JM, Zechiedrich L, Nadal M, Lamour V
Science (2024) 384 pp. 227-232 [ DOI: doi:10.1126/science.adl5899 Pubmed: 38603484 ]
- Dna gyrase subunit b (90 kDa, Protein from Escherichia coli)
- Dsdna minicircles (Complex from synthetic construct)
- Dna gyrase subunit a (97 kDa, Protein from Escherichia coli)
- E. coli dna gyrase in complex with dna minicircles (745 kDa, Complex)
- Dna minicircle (35 kDa, DNA from synthetic construct)
- Dna minicircle (36 kDa, DNA from synthetic construct)
- E. coli dna gyrase (Complex from Escherichia coli)
E. coli DNA gyrase bound to a DNA crossover
Vayssieres M, Marechal N, Yun L, Lopez Duran B, Murugasamy NK, Fogg JM, Zechiedrich L, Nadal M, Lamour V
Science (2024) 384 pp. 227-232 [ DOI: doi:10.1126/science.adl5899 Pubmed: 38603484 ]
Focused map of GyrA-CTD and T-segment DNA from the DNA crossover-gyrase complex
Vayssieres M, Marechal N, Yun L, Lopez Duran B, Murugasamy NK, Fogg JM, Zechiedrich L, Nadal M, Lamour V
Science (2024) 384 pp. 227-232 [ DOI: doi:10.1126/science.adl5899 Pubmed: 38603484 ]
Focused map of GyrA-CTD from DNA crossover-gyrase complex
Vayssieres M, Marechal N, Yun L, Lopez Duran B, Murugasamy NK, Fogg JM, Zechiedrich L, Nadal M, Lamour V
Science (2024) 384 pp. 227-232 [ DOI: doi:10.1126/science.adl5899 Pubmed: 38603484 ]
E. coli DNA gyrase bound to a linear part of a DNA minicircle
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8qqs
Q-score: 0.519
Vayssieres M, Marechal N, Yun L, Lopez Duran B, Murugasamy NK, Fogg JM, Zechiedrich L, Nadal M, Lamour V
Science (2024) 384 pp. 227-232 [ DOI: doi:10.1126/science.adl5899 Pubmed: 38603484 ]
- Dna (30-mer) (9 kDa, DNA from synthetic construct)
- Dna gyrase subunit b (90 kDa, Protein from Escherichia coli)
- E. coli dna gyrase in complex with dsdna minicircles (745 kDa, Complex)
- Dsdna minicircles (Complex from synthetic construct)
- Dna gyrase subunit a (97 kDa, Protein from Escherichia coli)
- Dna (30-mer) (9 kDa, DNA from synthetic construct)
- E. coli dna gyrase (Complex from Escherichia coli)
Asymetric subunit of E. coli DNA gyrase bound to a linear part of a DNA minicircle
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8qqu
Q-score: 0.542
Vayssieres M, Marechal N, Yun L, Lopez Duran B, Murugasamy NK, Fogg JM, Zechiedrich L, Nadal M, Lamour V
Science (2024) 384 pp. 227-232 [ DOI: doi:10.1126/science.adl5899 Pubmed: 38603484 ]
- Dna gyrase subunit b (90 kDa, Protein from Escherichia coli)
- E. coli dna gyrase (Complex from Escherichia coli)
- Dsdna minicircles (Complex from synthetic construct)
- Dna (16-mer) (4 kDa, DNA from synthetic construct)
- Dna gyrase subunit a (97 kDa, Protein from Escherichia coli)
- E. coli dna gyrase in complex with dsdna minicircles (745 kDa, Complex)
- Dna (16-mer) (4 kDa, DNA from synthetic construct)
Resolution: 4.09 Å
EM Method: Single-particle
Fitted PDBs: 8a43
Q-score: 0.337
Daiss JL, Pilsl M, Straub K, Bleckmann A, Hocherl M, Heiss FB, Abascal-Palacios G, Ramsay EP, Tluckova K, Mars JC, Furtges T, Bruckmann A, Rudack T, Bernecky C, Lamour V, Panov K, Vannini A, Moss T, Engel C
Life Sci Alliance (2022) 5 [ DOI: doi:10.26508/lsa.202201568 Pubmed: 36271492 ]
- Dna-directed rna polymerase i subunit rpa12 (13 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase i subunit rpa1 (195 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (24 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Homo sapiens)
- Rna polymerase i (Complex from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase i subunit rpa34 (55 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase i subunit rpa49 (47 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase i subunit rpa2 (128 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i and iii subunit rpac2 (15 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i and iii subunit rpac1 (39 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Homo sapiens)
Cryo-EM structure of the Human topoisomerase II alpha DNA-binding/cleavage domain in State 1
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6zy5
Q-score: 0.385
Vanden Broeck A, Lotz C, Drillien R, Haas L, Bedez C, Lamour V
Nat Commun (2021) 12 pp. 2962-2962 [ DOI: doi:10.1038/s41467-021-23136-6 Pubmed: 34016969 ]
- Dna topoisomerase 2-alpha (Complex from Homo sapiens)
- Dna (Complex from Homo sapiens)
- Etoposide-bound human top2a dna-binding/cleavage domain in state 1 (205 kDa, Complex)
- Dna (5'-d(*gp*ap*gp*gp*ap*tp*gp*ap*cp*gp*ap*tp*g)-3') (4 kDa, DNA from Homo sapiens)
- Dna topoisomerase 2-alpha (174 kDa, Protein from Homo sapiens)
- (5s,5ar,8ar,9r)-9-(4-hydroxy-3,5-dimethoxyphenyl)-8-oxo-5,5a,6,8,8a,9-hexahydrofuro[3',4':6,7]naphtho[2,3-d][1,3]dioxol -5-yl 4,6-o-[(1r)-ethylidene]-beta-d-glucopyranoside (588 Da, Ligand)
- Dna (5'-d(*cp*gp*cp*gp*cp*ap*tp*cp*gp*tp*cp*ap*tp*cp*cp*tp*c)-3') (5 kDa, DNA from Homo sapiens)
Cryo-EM structure of the Human topoisomerase II alpha DNA-binding/cleavage domain in State 2
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6zy6
Q-score: 0.272
Vanden Broeck A, Lotz C, Drillien R, Haas L, Bedez C, Lamour V
Nat Commun (2021) 12 pp. 2962-2962 [ DOI: doi:10.1038/s41467-021-23136-6 Pubmed: 34016969 ]
- Dna topoisomerase 2-alpha (Complex from Homo sapiens)
- Dna (Complex from Homo sapiens)
- Dna (5'-d(*gp*ap*gp*gp*ap*tp*gp*ap*cp*gp*ap*tp*g)-3') (4 kDa, DNA from Homo sapiens)
- Dna topoisomerase 2-alpha (174 kDa, Protein from Homo sapiens)
- (5s,5ar,8ar,9r)-9-(4-hydroxy-3,5-dimethoxyphenyl)-8-oxo-5,5a,6,8,8a,9-hexahydrofuro[3',4':6,7]naphtho[2,3-d][1,3]dioxol -5-yl 4,6-o-[(1r)-ethylidene]-beta-d-glucopyranoside (588 Da, Ligand)
- Etoposide-bound human top2a dna-binding/cleavage domain in state 2 (205 kDa, Complex)
- Dna (5'-d(*cp*gp*cp*gp*cp*ap*tp*cp*gp*tp*cp*ap*tp*cp*cp*tp*c)-3') (5 kDa, DNA from Homo sapiens)
Entire Human Top2a - Interface between ATPase and DNA-binding/cleavage domains
Vanden Broeck A, Lotz C, Drillien R, Haas L, Bedez C, Lamour V
Nat Commun (2021) 12 pp. 2962-2962 [ DOI: doi:10.1038/s41467-021-23136-6 Pubmed: 34016969 ]
- Etoposide-bound entire human top2a (280 kDa, Complex from Homo sapiens)
- Human top2a (Protein from Homo sapiens)