Cryo-EM movies of the human α1β3γ2 GABAA receptor in a lipid bilayer bound to diazepam and GABA
Masiulis S, Desai R, Uchański T, Serna Martin I, Laverty D, Karia D, Malinauskas T, Zivanov J, Pardon E, Kotecha A, Steyaert J, Miller KW, Aricescu AR
Nature (2019) 565 pp. 454-459 [ Pubmed: 30602790 DOI: 10.1038/s41586-018-0832-5 ]
Image sets:
Cryo-EM movies of the human α1β3γ2 GABAA receptor in a lipid bilayer bound to bicuculline
Masiulis S, Desai R, Uchański T, Serna Martin I, Laverty D, Karia D, Malinauskas T, Zivanov J, Pardon E, Kotecha A, Steyaert J, Miller KW, Aricescu AR
Nature (2019) 565 pp. 454-459 [ Pubmed: 30602790 DOI: 10.1038/s41586-018-0832-5 ]
Image sets:
Cryo-EM movies of the human α1β3γ2 GABAA receptor in a lipid bilayer bound to alprazolam and GABA
Masiulis S, Desai R, Uchański T, Serna Martin I, Laverty D, Karia D, Malinauskas T, Zivanov J, Pardon E, Kotecha A, Steyaert J, Miller KW, Aricescu AR
Nature (2019) 565 pp. 454-459 [ Pubmed: 30602790 DOI: 10.1038/s41586-018-0832-5 ]
Image sets:
Cryo-EM movies of the human α1β3γ2 GABAA receptor in a lipid bilayer bound to picrotoxin and GABA
Masiulis S, Desai R, Uchański T, Serna Martin I, Laverty D, Karia D, Malinauskas T, Zivanov J, Pardon E, Kotecha A, Steyaert J, Miller KW, Aricescu AR
Nature (2019) 565 pp. 454-459 [ Pubmed: 30602790 DOI: 10.1038/s41586-018-0832-5 ]
Image sets:
Cryo-EM movies of the human α1β3γ2 GABAA receptor in a lipid bilayer
Laverty D, Desai R, Uchański T, Masiulis S, Stec WJ, Malinauskas T, Zivanov J, Pardon E, Steyaert J, Miller KW, Aricescu AR
Nature (2019) 565 pp. 516-520 [ DOI: 10.1038/s41586-018-0833-4 Pubmed: 30602789 ]
Image sets:
Cryo-EM movies of the human α1β3γ2 GABAA receptor in a lipid bilayer bound to picrotoxin
Masiulis S, Desai R, Uchański T, Serna Martin I, Laverty D, Karia D, Malinauskas T, Zivanov J, Pardon E, Kotecha A, Steyaert J, Miller KW, Aricescu AR
Nature (2019) 565 pp. 454-459 [ Pubmed: 30602790 DOI: 10.1038/s41586-018-0832-5 ]
Image sets:
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6hug
Q-score: 0.55
Masiulis S, Desai R, Uchanski T, Serna Martin I, Laverty D, Karia D, Malinauskas T, Zivanov J, Pardon E, Kotecha A, Steyaert J, Miller KW, Aricescu AR
Nature (2019) 565 pp. 454-459 [ DOI: doi:10.1038/s41586-018-0832-5 Pubmed: 30602790 ]
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Human full-length heteromeric alpha1beta3gamma2l gaba(a)r in complex with picrotoxin and megabody mb38. (Complex from Homo sapiens)
- (1ar,2ar,3s,6r,6as,8as,8br,9r)-2a-hydroxy-8b-methyl-9-(prop-1-en-2-yl)hexahydro-3,6-methano-1,5,7-trioxacyclopenta[ij]c yclopropa[a]azulene-4,8(3h)-dione (292 Da, Ligand)
- Human full-length heteromeric alpha1beta3gamma2l gaba(a)r in complex with picrotoxin and megabody mb38. (330 kDa, Complex)
- Gamma-aminobutyric acid receptor subunit gamma-2 (56 kDa, Protein from Homo sapiens)
- Gamma-aminobutyric acid receptor subunit beta-3 (54 kDa, Protein from Homo sapiens)
- Human full-length heteromeric alpha1beta3gamma2l gaba(a)r in complex with picrotoxin and megabody mb38. (Complex from Lama glama)
- Gamma-aminobutyric acid receptor subunit alpha-1 (49 kDa, Protein from Bos taurus)
- Megabody mb38 (57 kDa, Protein from Lama glama)
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 6huj
Q-score: 0.541
Masiulis S, Desai R, Uchanski T, Serna Martin I, Laverty D, Karia D, Malinauskas T, Zivanov J, Pardon E, Kotecha A, Steyaert J, Miller KW, Aricescu AR
Nature (2019) 565 pp. 454-459 [ DOI: doi:10.1038/s41586-018-0832-5 Pubmed: 30602790 ]
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- (1ar,2ar,3s,6r,6as,8as,8br,9r)-2a-hydroxy-8b-methyl-9-(prop-1-en-2-yl)hexahydro-3,6-methano-1,5,7-trioxacyclopenta[ij]c yclopropa[a]azulene-4,8(3h)-dione (292 Da, Ligand)
- Gamma-amino-butanoic acid (103 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit gamma-2 (56 kDa, Protein from Homo sapiens)
- Gamma-aminobutyric acid receptor subunit beta-3 (54 kDa, Protein from Homo sapiens)
- Human full-length heteromeric alpha1beta3gamma2l gaba(a)r in complex with picrotoxin, gaba and megabody mb38. (330 kDa, Complex)
- Megabody mb38 (Complex from Lama glama)
- Gamma-aminobutyric acid receptor subunit beta-3, gamma-aminobutyric acid receptor subunit gamma-2 (Complex from Homo sapiens)
- Gamma-aminobutyric acid receptor subunit alpha-1,gamma-aminobutyric acid receptor subunit alpha-1 (52 kDa, Protein from Homo sapiens)
- Megabody mb38 (57 kDa, Protein from Lama glama)
Resolution: 3.69 Å
EM Method: Single-particle
Fitted PDBs: 6huk
Q-score: 0.455
Masiulis S, Desai R, Uchanski T, Serna Martin I, Laverty D, Karia D, Malinauskas T, Zivanov J, Pardon E, Kotecha A, Steyaert J, Miller KW, Aricescu AR
Nature (2019) 565 pp. 454-459 [ DOI: doi:10.1038/s41586-018-0832-5 Pubmed: 30602790 ]
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Human full-length heteromeric alpha1beta3gamma2l gaba(a)r in complex with bicuculline and megabody mb38. (330 kDa, Complex)
- Gamma-aminobutyric acid receptor subunit gamma-2 (56 kDa, Protein from Homo sapiens)
- Gamma-aminobutyric acid receptor subunit beta-3 (54 kDa, Protein from Homo sapiens)
- Megabody mb38 (Complex from Lama glama)
- Bicuculline methochloride (367 Da, Ligand)
- Human full-length heteromeric alpha1beta3gamma2l gaba(a)r (Complex from Homo sapiens)
- Gamma-aminobutyric acid receptor subunit alpha-1,gamma-aminobutyric acid receptor subunit alpha-1 (52 kDa, Protein from Homo sapiens)
- Megabody mb38 (57 kDa, Protein from Lama glama)
Resolution: 3.26 Å
EM Method: Single-particle
Fitted PDBs: 6huo
Q-score: 0.514
Masiulis S, Desai R, Uchanski T, Serna Martin I, Laverty D, Karia D, Malinauskas T, Zivanov J, Pardon E, Kotecha A, Steyaert J, Miller KW, Aricescu AR
Nature (2019) 565 pp. 454-459 [ DOI: doi:10.1038/s41586-018-0832-5 Pubmed: 30602790 ]
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Gamma-amino-butanoic acid (103 Da, Ligand)
- Cryoem structure of human full-length heteromeric alpha1beta3gamma2l gaba(a)r in complex with alprazolam (xanax), gaba and megabody mb38. (330 kDa, Complex)
- Gamma-aminobutyric acid receptor subunit gamma-2 (56 kDa, Protein from Homo sapiens)
- Gamma-aminobutyric acid receptor subunit beta-3 (54 kDa, Protein from Homo sapiens)
- Megabody mb38 (Complex from Lama glama)
- Heteromeric alpha1beta3gamma2l gaba(a)r (Complex from Homo sapiens)
- Gamma-aminobutyric acid receptor subunit alpha-1,gamma-aminobutyric acid receptor subunit alpha-1 (52 kDa, Protein from Homo sapiens)
- 8-chloro-1-methyl-6-phenyl-4h-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine (308 Da, Ligand)
- Megabody mb38 (57 kDa, Protein from Lama glama)