Cryo-EM structure of RNA device 43, holo state
Stagno JR, Deme JC, Lee Y-T, Wang Y-X, Lea SM
To Be Published
- Magnesium ion (24 Da, Ligand)
- Synthetic rna device containing hammerhead ribozyme and tetracycline-binding aptamer (40 kDa, Complex from synthetic construct)
- Water (18 Da, Ligand)
- Tetracycline (444 Da, Ligand)
- Rna device 43 (40 kDa, RNA from synthetic construct)
Cryo-EM structure of RNA device 43, apo state
Stagno JR, Deme JC, Lee Y-T, Wang Y-X, Lea SM
To Be Published
- Magnesium ion (24 Da, Ligand)
- Synthetic rna device containing hammerhead ribozyme and tetracycline-binding aptamer (40 kDa, Complex from synthetic construct)
- Rna device 43 (40 kDa, RNA from synthetic construct)
Cryo-EM structure of RNA device 43 truncation mutant 3 (U100C), apo state
Stagno JR, Deme JC, Lee Y-T, Wang Y-X, Lea SM
To Be Published
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Synthetic rna device containing hammerhead ribozyme and tetracycline-binding aptamer (35 kDa, Complex from synthetic construct)
- Rna device 43 truncation mutant 3 (u100c) (34 kDa, RNA from synthetic construct)
Cryo-EM structure of a type II ZorAB complex from Sulfuricurvum kujiense
Cryo-EM structure of a type I ZorAB complex from Shewanella sp. strain ANA-3, composite map
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 8vvn
Q-score: 0.595
Mariano G, Deme JC, Blower TR, Palmer T, Lea SM
Nat Commun (2025) [ ]
- Water (18 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Chemotaxis protein motb-related protein (32 kDa, Protein from Shewanella sp. ANA-3)
- Zorab complex (Complex from Shewanella sp. ANA-3)
- Mota/tolq/exbb proton channel domain-containing protein (77 kDa, Protein from Shewanella sp. ANA-3)
Cryo-EM structure of the mammalian peptide transporter PepT2 bound to cefadroxil
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 9bir
Q-score: 0.549
Parker JL, Deme JC, Lichtinger SM, Kuteyi G, Biggin PC, Lea SM, Newstead S
Nat Commun (2024) 15 pp. 8755-8755 [ Pubmed: 39384780 DOI: doi:10.1038/s41467-024-53096-6 ]
- Nanobody (Complex from Lama glama)
- Complex of the mammalian peptide transporter pept2 with nanobody and bound cefadroxil (Complex from Rattus norvegicus)
- Cefadroxil (363 Da, Ligand)
- Solute carrier family 15 member 2 (82 kDa, Protein from Rattus norvegicus)
- Pept2 (Complex from Rattus norvegicus)
- Nanobody (14 kDa, Protein from Lama glama)
Cryo-EM structure of the mammalian peptide transporter PepT2 bound to amoxicillin
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 9bis
Q-score: 0.523
Parker JL, Deme JC, Lichtinger SM, Kuteyi G, Biggin PC, Lea SM, Newstead S
Nat Commun (2024) 15 pp. 8755-8755 [ Pubmed: 39384780 DOI: doi:10.1038/s41467-024-53096-6 ]
- Nanobody (Complex from Lama glama)
- Solute carrier family 15 member 2 (82 kDa, Protein from Rattus norvegicus)
- Pept2 (Complex from Rattus norvegicus)
- Complex of the mammalian peptide transporter pept2 with nanobody and bound amoxicillin (Complex from Rattus norvegicus)
- 2-{1-[2-amino-2-(4-hydroxy-phenyl)-acetylamino]-2-oxo-ethyl}-5,5-dimethyl-thiazolidine-4-carboxylic acid (367 Da, Ligand)
- Nanobody (14 kDa, Protein from Lama glama)
Cryo-EM structure of the mammalian peptide transporter PepT2 bound to cloxacillin, pose 1
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 9bit
Q-score: 0.539
Parker JL, Deme JC, Lichtinger SM, Kuteyi G, Biggin PC, Lea SM, Newstead S
Nat Commun (2024) 15 pp. 8755-8755 [ Pubmed: 39384780 DOI: doi:10.1038/s41467-024-53096-6 ]
- Cloxacillin (435 Da, Ligand)
- Complex of the mammalian peptide transporter pept2 with nanobody and bound cloxacillin, pose 1 (Complex from Rattus norvegicus)
- Nanobody (Complex from Lama glama)
- Solute carrier family 15 member 2 (82 kDa, Protein from Rattus norvegicus)
- Pept2 (Complex from Rattus norvegicus)
- Nanobody (14 kDa, Protein from Lama glama)