Cryo-EM structure of RNase-treated RESC-A in trypanosomal RNA editing
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8fn4
Q-score: 0.416
Liu S, Wang H, Li X, Zhang F, Lee JKJ, Li Z, Yu C, Hu JJ, Zhao X, Suematsu T, Alvarez-Cabrera AL, Liu Q, Zhang L, Huang L, Aphasizheva I, Aphasizhev R, Zhou ZH
Science (2023) 381 pp. eadg4725-eadg4725 [ Pubmed: 37410820 DOI: doi:10.1126/science.adg4725 ]
- Resc5-tagged isolate with rnase treatment (Complex from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 3 (resc3) (53 kDa, Protein from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 1 (resc1) (52 kDa, Protein from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 4 (resc4) (120 kDa, Protein from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 2 (resc2) (55 kDa, Protein from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 5 (resc5) (43 kDa, Protein from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 6 (resc6) (57 kDa, Protein from Trypanosoma brucei)
Cryo-EM structure of RNase-untreated RESC-A in trypanosomal RNA editing
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8fn6
Q-score: 0.386
Liu S, Wang H, Li X, Zhang F, Lee JKJ, Li Z, Yu C, Hu JJ, Zhao X, Suematsu T, Alvarez-Cabrera AL, Liu Q, Zhang L, Huang L, Aphasizheva I, Aphasizhev R, Zhou ZH
Science (2023) 381 pp. eadg4725-eadg4725 [ Pubmed: 37410820 DOI: doi:10.1126/science.adg4725 ]
- Rna-editing substrate-binding complex protein 3 (resc3) (53 kDa, Protein from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 1 (resc1) (52 kDa, Protein from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 4 (resc4) (120 kDa, Protein from Trypanosoma brucei)
- Grna (14 kDa, RNA from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 2 (resc2) (55 kDa, Protein from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 5 (resc5) (43 kDa, Protein from Trypanosoma brucei)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Resc5-tagged isolate without rnase treatment (Complex from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 6 (resc6) (57 kDa, Protein from Trypanosoma brucei)
Cryo-EM structure of RNase-treated RESC-C in trypanosomal RNA editing
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8fnc
Q-score: 0.533
Liu S, Wang H, Li X, Zhang F, Lee JKJ, Li Z, Yu C, Hu JJ, Zhao X, Suematsu T, Alvarez-Cabrera AL, Liu Q, Zhang L, Huang L, Aphasizheva I, Aphasizhev R, Zhou ZH
Science (2023) 381 pp. eadg4725-eadg4725 [ Pubmed: 37410820 DOI: doi:10.1126/science.adg4725 ]
- Resc5-tagged isolate with rnase treatment (Complex from Trypanosoma brucei)
- Mrna (5 kDa, RNA from Trypanosoma brucei)
- Grna (4 kDa, RNA from Trypanosoma brucei)
- Rxlr effector protein (18 kDa, Protein from Trypanosoma brucei)
- Mitochondrial rna binding complex 1 subunit (61 kDa, Protein from Trypanosoma brucei)
- Rap domain-containing protein (60 kDa, Protein from Trypanosoma brucei)
- Phytanoyl-coa dioxygenase family protein (41 kDa, Protein from Trypanosoma brucei)
- Mitochondrial rna binding protein (34 kDa, Protein from Trypanosoma brucei)
- Rap domain-containing protein (57 kDa, Protein from Trypanosoma brucei)
Cryo-EM structure of RNase-untreated RESC-C in trypanosomal RNA editing
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8fnf
Q-score: 0.448
Liu S, Wang H, Li X, Zhang F, Lee JKJ, Li Z, Yu C, Hu JJ, Zhao X, Suematsu T, Alvarez-Cabrera AL, Liu Q, Zhang L, Huang L, Aphasizheva I, Aphasizhev R, Zhou ZH
Science (2023) 381 pp. eadg4725-eadg4725 [ Pubmed: 37410820 DOI: doi:10.1126/science.adg4725 ]
- Grna (4 kDa, RNA from Trypanosoma brucei)
- Rxlr effector protein (18 kDa, Protein from Trypanosoma brucei)
- Mitochondrial rna binding complex 1 subunit (61 kDa, Protein from Trypanosoma brucei)
- Rap domain-containing protein (60 kDa, Protein from Trypanosoma brucei)
- Mrna (6 kDa, RNA from Trypanosoma brucei)
- Resc5-tagged isolate without rnase treatment (Complex from Trypanosoma brucei)
- Phytanoyl-coa dioxygenase family protein (41 kDa, Protein from Trypanosoma brucei)
- Mitochondrial rna binding protein (34 kDa, Protein from Trypanosoma brucei)
- Rap domain-containing protein (57 kDa, Protein from Trypanosoma brucei)
Cryo-EM structure of RNase-treated RESC-B in trypanosomal RNA editing
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8fni
Q-score: 0.443
Liu S, Wang H, Li X, Zhang F, Lee JKJ, Li Z, Yu C, Hu JJ, Zhao X, Suematsu T, Alvarez-Cabrera AL, Liu Q, Zhang L, Huang L, Aphasizheva I, Aphasizhev R, Zhou ZH
Science (2023) 381 pp. eadg4725-eadg4725 [ Pubmed: 37410820 DOI: doi:10.1126/science.adg4725 ]
- Resc5-tagged isolate with rnase treatment (Complex from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 10 (resc10) (60 kDa, Protein from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 9 (resc9) (96 kDa, Protein from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 13 (resc13) (32 kDa, Protein from Trypanosoma brucei)
- Mrna (6 kDa, RNA from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 11 (resc11) (103 kDa, Protein from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 5 (resc5) (43 kDa, Protein from Trypanosoma brucei)
- Grna (4 kDa, RNA from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 7 (resc7) (18 kDa, Protein from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 14 (resc14) (41 kDa, Protein from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 8 (resc8) (61 kDa, Protein from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 6 (resc6) (57 kDa, Protein from Trypanosoma brucei)
Cryo-EM structure of RNase-untreated RESC-B in trypanosomal RNA editing
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8fnk
Q-score: 0.37
Liu S, Wang H, Li X, Zhang F, Lee JKJ, Li Z, Yu C, Hu JJ, Zhao X, Suematsu T, Alvarez-Cabrera AL, Liu Q, Zhang L, Huang L, Aphasizheva I, Aphasizhev R, Zhou ZH
Science (2023) 381 pp. eadg4725-eadg4725 [ Pubmed: 37410820 DOI: doi:10.1126/science.adg4725 ]
- Mrna (15 kDa, RNA from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 9 (resc9) (96 kDa, Protein from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 10 (resc10) (60 kDa, Protein from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 13 (resc13) (32 kDa, Protein from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 11 (resc11) (103 kDa, Protein from Trypanosoma brucei)
- Grna (13 kDa, RNA from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 5 (resc5) (43 kDa, Protein from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 7 (resc7) (18 kDa, Protein from Trypanosoma brucei)
- Resc5-tagged isolate without rnase treatment (Complex from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 14 (resc14) (41 kDa, Protein from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 8 (resc8) (61 kDa, Protein from Trypanosoma brucei)
- Rna-editing substrate-binding complex protein 6 (resc6) (57 kDa, Protein from Trypanosoma brucei)
3-methylcrotonyl-CoA carboxylase in filament, beta-subunit centered
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8f3d
Q-score: 0.317
Hu JJ, Lee JKJ, Liu YT, Yu C, Huang L, Aphasizheva I, Aphasizhev R, Zhou ZH
Structure (2023) 31 pp. 100-110.e4 [ DOI: doi:10.1016/j.str.2022.11.015 Pubmed: 36543169 ]
- 3-methylcrotonyl-coa carboxylase alpha-subunit (76 kDa, Protein from Leishmania tarentolae)
- 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal (228 Da, Ligand)
- Alpha6beta6 dodecamer in 3-methylcrotonyl-coa filament (Complex from Leishmania tarentolae)
- 3-methylcrotonyl-coa carboxylase beta-subunit (76 kDa, Protein from Leishmania tarentolae)
3-methylcrotonyl-CoA carboxylase in filaments, filament termini
Hu JJ, Lee JKJ, Liu YT, Yu C, Huang L, Aphasizheva I, Aphasizhev R, Zhou ZH
Structure (2023) 31 pp. 100-110.e4 [ DOI: doi:10.1016/j.str.2022.11.015 Pubmed: 36543169 ]
3-methylcrotonyl-CoA carboxylase in filament, alpha-subunit centered
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8f41
Q-score: 0.29
Hu JJ, Lee JKJ, Liu YT, Yu C, Huang L, Aphasizheva I, Aphasizhev R, Zhou ZH
Structure (2023) 31 pp. 100-110.e4 [ DOI: doi:10.1016/j.str.2022.11.015 Pubmed: 36543169 ]
- 3-methylcrotonyl-coa carboxylase, alpha-subunit (75 kDa, Protein from Leishmania tarentolae)
- 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal (228 Da, Ligand)
- Alpha6beta6 dodecamer in 3-methylcrotonyl-coa carboxylase filament (Complex from Leishmania tarentolae)
- 3-methylcrotonyl-coa carboxylase, beta-subunit (61 kDa, Protein from Leishmania tarentolae)