Human TRiC-PhLP2A complex in the closed state
Resolution: 3.24 Å
EM Method: Single-particle
Fitted PDBs: 8i1u
Q-score: 0.506
Park J, Kim H, Gestaut D, Lim S, Opoku-Nsiah KA, Leitner A, Frydman J, Roh SH
Nat Commun (2024) 15 pp. 1007-1007 [ DOI: doi:10.1038/s41467-024-45242-x Pubmed: 38307855 ]
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Aluminum fluoride (83 Da, Ligand)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- Complex of tric/cct-phlp2a treated with atp-alfx (1 MDa, Complex from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- Human tric/cct complex (Complex from Homo sapiens)
- Phlp2a (pdcl3) (Complex)
- T-complex protein 1 subunit eta (59 kDa, Protein from Homo sapiens)
- Phosducin-like protein 3 (27 kDa, Protein from Homo sapiens)
The focused refinement of CCT3-PhLP2A from TRiC-PhLP2A complex in the open state
Resolution: 3.82 Å
EM Method: Single-particle
Fitted PDBs: 8i6j
Q-score: 0.404
Park J, Kim H, Gestaut D, Lim S, Opoku-Nsiah KA, Leitner A, Frydman J, Roh SH
Nat Commun (2024) 15 pp. 1007-1007 [ DOI: doi:10.1038/s41467-024-45242-x Pubmed: 38307855 ]
- Tric/cct (Complex)
- Phlp2a (pdcl3) (Complex from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- Complex of tric/cct and phlp2a (1 MDa, Complex from Homo sapiens)
- Phosducin-like protein 3 (27 kDa, Protein from Homo sapiens)
Human TRiC-PhLP2A complex in the open state
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8i9u
Q-score: 0.286
Park J, Kim H, Gestaut D, Lim S, Opoku-Nsiah KA, Leitner A, Frydman J, Roh SH
Nat Commun (2024) 15 pp. 1007-1007 [ DOI: doi:10.1038/s41467-024-45242-x Pubmed: 38307855 ]
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- Human tric/cct complex (Complex from Homo sapiens)
- T-complex protein 1 subunit eta (59 kDa, Protein from Homo sapiens)
- Phlp2a (pdcl3) in human tric/cct complex (Complex from Homo sapiens)
- Phosducin-like protein 3 (27 kDa, Protein from Homo sapiens)
- Complex of human tric/cct and phlp2a (1 MDa, Complex)
The focused refinement of CCT4-PhLP2A from TRiC-PhLP2A complex in the open state
Resolution: 4.22 Å
EM Method: Single-particle
Fitted PDBs: 8i9q
Q-score: 0.318
Park J, Kim H, Gestaut D, Lim S, Opoku-Nsiah KA, Leitner A, Frydman J, Roh SH
Nat Commun (2024) 15 pp. 1007-1007 [ DOI: doi:10.1038/s41467-024-45242-x Pubmed: 38307855 ]
- Phlp2a (pdcl3) (Complex from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit delta from human tric/cct complex (Complex from Homo sapiens)
- Complex of human tric/cct-phlp2a (1 MDa, Complex)
- Phosducin-like protein 3 (27 kDa, Protein from Homo sapiens)
Human TRiC-PhLP2A-actin complex in the closed state
Resolution: 4.42 Å
EM Method: Single-particle
Fitted PDBs: 8ib8
Q-score: 0.271
Park J, Kim H, Gestaut D, Lim S, Opoku-Nsiah KA, Leitner A, Frydman J, Roh SH
Nat Commun (2024) 15 pp. 1007-1007 [ DOI: doi:10.1038/s41467-024-45242-x Pubmed: 38307855 ]
- Actb protein (fragment) (41 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- Phlp2a (pdcl3) (Complex from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- Human tric/cct complex (Complex from Homo sapiens)
- T-complex protein 1 subunit eta (59 kDa, Protein from Homo sapiens)
- Complex of tric/cct, phlp2a, and actin treated with atp-alfx (1 MDa, Complex)
- Human actin (Complex from Homo sapiens)
- Phosducin-like protein 3 (27 kDa, Protein from Homo sapiens)
Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell (2022) 185 pp. 4770-4787.e20 [ DOI: doi:10.1016/j.cell.2022.11.014 Pubmed: 36493755 ]
- Cct subunit 1 (Macromolecule from Homo sapiens)
- Cct subunit 2 (Macromolecule from Homo sapiens)
- Cct subunit 5 (Macromolecule from Homo sapiens)
- Cct subunit 3 (Macromolecule from Homo sapiens)
- Cct subunit 8 (Macromolecule from Homo sapiens)
- Apo tric/cct (1 MDa, Complex from Homo sapiens)
- Cct subunit 7 (Macromolecule from Homo sapiens)
- Cct subunit 6 (Macromolecule from Homo sapiens)
- Cct subunit 4 (Macromolecule from Homo sapiens)
The beta-tubulin folding intermediate I
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7trg
Q-score: 0.549
Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell (2022) 185 pp. 4770-4787.e20 [ DOI: doi:10.1016/j.cell.2022.11.014 Pubmed: 36493755 ]
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Aluminum fluoride (83 Da, Ligand)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- T-complex protein 1 subunit eta (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- Closed form human tric in complex with beta-tubulin under atp/alf3condition. (1 MDa, Complex from Homo sapiens)
The beta-tubulin folding intermediate II
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7ttn
Q-score: 0.526
Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell (2022) 185 pp. 4770-4787.e20 [ DOI: doi:10.1016/j.cell.2022.11.014 Pubmed: 36493755 ]
- Closed form human tric in complex with beta-tubulin under atp/alfx condition. (1 MDa, Complex from Homo sapiens)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- Aluminum fluoride (83 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit eta (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
The beta-tubulin folding intermediate III
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7ttt
Q-score: 0.561
Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell (2022) 185 pp. 4770-4787.e20 [ DOI: doi:10.1016/j.cell.2022.11.014 Pubmed: 36493755 ]
- Closed form human tric in complex with beta-tubulin under atp/alfx condition. (1 MDa, Complex from Homo sapiens)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Aluminum fluoride (83 Da, Ligand)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit eta (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
The beta-tubulin folding intermediate IV
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7tub
Q-score: 0.5
Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell (2022) 185 pp. 4770-4787.e20 [ DOI: doi:10.1016/j.cell.2022.11.014 Pubmed: 36493755 ]
- Closed form human tric in complex with beta-tubulin under atp/alfx condition. (1 MDa, Complex from Homo sapiens)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Aluminum fluoride (83 Da, Ligand)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit eta (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)