Structural basis of COMPASS eCM recognition of an unmodified nucleosome
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6ugm
Q-score: 0.385
Hsu PL, Shi H, Leonen C, Kang J, Chatterjee C, Zheng N
Mol Cell (2019) 76 pp. 712- [ DOI: doi:10.1016/j.molcel.2019.10.013 Pubmed: 31733991 ]
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Bre2 (47 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Dna (146-mer) (45 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Yeast compass ecm bound to an unmodified nucleosome (450 kDa, Complex)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Histone-lysine n-methyltransferase, h3 lysine-4 specific (31 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Nucleosome (Complex from Xenopus laevis)
- Swd1 (49 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Histone h2a (11 kDa, Protein from Xenopus laevis)
- Dna (146-mer) (45 kDa, DNA from synthetic construct)
- Spp1 (39 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Swd3 (36 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- H3 n-terminus (378 Da, Protein from Xenopus laevis)
- Histone h2b (13 kDa, Protein from Xenopus laevis)
- S-adenosylmethionine (398 Da, Ligand)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Sdc1 (14 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Compass ecm (Complex from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
Structural basis of COMPASS eCM recognition of the H2Bub nucleosome
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6uh5
Q-score: 0.372
Hsu PL, Shi H, Leonen C, Kang J, Chatterjee C, Zheng N
Mol Cell (2019) 76 pp. 712- [ DOI: doi:10.1016/j.molcel.2019.10.013 Pubmed: 31733991 ]
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Bre2 (47 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Ubiquitin (Complex from Homo sapiens)
- Dna (146-mer) (45 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Structural basis of compass ecm recognition of the h2bub nucleosome (450 kDa, Complex)
- Histone-lysine n-methyltransferase, h3 lysine-4 specific (31 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- H3 n-terminus (936 Da, Protein from Xenopus laevis)
- Nucleosome (Complex from Xenopus laevis)
- Ubiquitin (8 kDa, Protein from Homo sapiens)
- Swd1 (49 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Histone h2a (11 kDa, Protein from Xenopus laevis)
- Spp1 (39 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Swd3 (36 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Histone h2b (13 kDa, Protein from Xenopus laevis)
- Histone h2b (13 kDa, Protein from Xenopus laevis)
- Dna (146-mer) (44 kDa, DNA from synthetic construct)
- S-adenosylmethionine (398 Da, Ligand)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Sdc1 (14 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Compass ecm (Complex from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))