cryo-EM structure of NTSR1-GRK2-Galpha(q) complexes 1
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 8jpb
Q-score: 0.448
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ DOI: doi:10.1038/s41586-023-06395-9 Pubmed: 37532940 ]
- Beta-adrenergic receptor kinase 1 (79 kDa, Protein from Bos taurus)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanine nucleotide-binding protein g(q) subunit alpha (41 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Tetrafluoroaluminate ion (102 Da, Ligand)
- 2-[{2-(1-fluorocyclopropyl)-4-[4-(2-methoxyphenyl)piperidin-1-yl]quinazolin-6-yl}(methyl)amino]ethan-1-ol (450 Da, Ligand)
- Staurosporine (466 Da, Ligand)
- Ntsr1-grk2-galpha(q) complexes 1 (Complex from Homo sapiens)
- Nts(8-13) (819 Da, Protein from synthetic construct)
- Neurotensin receptor type 1 (46 kDa, Protein from Homo sapiens)
cryo-EM structure of NTSR1-GRK2-Galpha(q) complexes 2
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 8jpc
Q-score: 0.458
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ DOI: doi:10.1038/s41586-023-06395-9 Pubmed: 37532940 ]
- Beta-adrenergic receptor kinase 1 (79 kDa, Protein from Bos taurus)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanine nucleotide-binding protein g(q) subunit alpha (41 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Tetrafluoroaluminate ion (102 Da, Ligand)
- 2-[{2-(1-fluorocyclopropyl)-4-[4-(2-methoxyphenyl)piperidin-1-yl]quinazolin-6-yl}(methyl)amino]ethan-1-ol (450 Da, Ligand)
- Staurosporine (466 Da, Ligand)
- Ntsr1-grk2-galpha(q) complexes 2 (Complex from Homo sapiens)
- Nts(8-13) (819 Da, Protein from synthetic construct)
- Neurotensin receptor type 1 (46 kDa, Protein from Homo sapiens)
Focused refinement structure of GRK2 in NTSR1-GRK2-Galpha(q) complexes
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 8jpd
Q-score: 0.497
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ DOI: doi:10.1038/s41586-023-06395-9 Pubmed: 37532940 ]
- Focused refinement structure of grk2 in ntsr1-grk2-galpha(q) complexes (Complex from Bos taurus)
- Staurosporine (466 Da, Ligand)
- Beta-adrenergic receptor kinase 1 (79 kDa, Protein from Bos taurus)
Focused refinement structure of Galpha(q) in NTSR1-GRK2-Galpha(q) complexes
Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 8jpe
Q-score: 0.447
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ DOI: doi:10.1038/s41586-023-06395-9 Pubmed: 37532940 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Guanine nucleotide-binding protein g(q) subunit alpha (41 kDa, Protein from Homo sapiens)
- Focused refinement structure of galpha(q) in ntsr1-grk2-galpha(q) complexes (Complex from Homo sapiens)
- Tetrafluoroaluminate ion (102 Da, Ligand)
Focused refiment structure of NTSR1 in NTSR1-GRK2-Galpha(q) complexes
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 8jpf
Q-score: 0.479
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ DOI: doi:10.1038/s41586-023-06395-9 Pubmed: 37532940 ]
- Focused refiment structure of ntsr1 in ntsr1-grk2-galpha(q) complexes (Complex from Homo sapiens)
- 2-[{2-(1-fluorocyclopropyl)-4-[4-(2-methoxyphenyl)piperidin-1-yl]quinazolin-6-yl}(methyl)amino]ethan-1-ol (450 Da, Ligand)
- Nts (819 Da, Protein from synthetic construct)
- Neurotensin receptor type 1 (46 kDa, Protein from Homo sapiens)