Cryo-EM structure of SNAP-94847-bound MCHR1, S1 state
Resolution: 3.33 Å
EM Method: Single-particle
Fitted PDBs: 8yns
Q-score: 0.476
Ye X, Liu G, Li X, He B, Tao Y, Guan J, Mu Y, Liu H, Gong W
Cell Discov (2024) 10 pp. 124-124 [ DOI: doi:10.1038/s41421-024-00754-0 Pubmed: 39616153 ]
- Glue molecule 4-9 (30 kDa, Protein from synthetic construct)
- Snap-94847-mchr1-fab1b3-glue complex (Complex from Homo sapiens)
- Melanin-concentrating hormone receptor 1,soluble cytochrome b562 (64 kDa, Protein from Homo sapiens)
- Antibody 1b3 fab light chain (23 kDa, Protein from synthetic construct)
- Antibody 1b3 fab heavy chain (25 kDa, Protein from synthetic construct)
- ~{n}-[3-[1-[[4-[3,4-bis(fluoranyl)phenoxy]phenyl]methyl]piperidin-4-yl]-4-methyl-phenyl]-2-methyl-propanamide (478 Da, Ligand)
Cryo-EM structure of SNAP-94847-bound MCHR1, S2 state
Resolution: 3.43 Å
EM Method: Single-particle
Fitted PDBs: 8ynt
Q-score: 0.422
Ye X, Liu G, Li X, He B, Tao Y, Guan J, Mu Y, Liu H, Gong W
Cell Discov (2024) 10 pp. 124-124 [ DOI: doi:10.1038/s41421-024-00754-0 Pubmed: 39616153 ]
- Glue molecule 4-9 (30 kDa, Protein from synthetic construct)
- Snap-94847-mchr1-fab1b3-glue complex (Complex from Homo sapiens)
- Melanin-concentrating hormone receptor 1,soluble cytochrome b562 (64 kDa, Protein from Homo sapiens)
- Antibody 1b3 fab light chain (23 kDa, Protein from synthetic construct)
- Antibody 1b3 fab heavy chain (25 kDa, Protein from synthetic construct)
- ~{n}-[3-[1-[[4-[3,4-bis(fluoranyl)phenoxy]phenyl]methyl]piperidin-4-yl]-4-methyl-phenyl]-2-methyl-propanamide (478 Da, Ligand)
MCH-MCHR1-Gi complex, T1 state
Resolution: 2.61 Å
EM Method: Single-particle
Fitted PDBs: 8wwk
Q-score: 0.611
Ye X, Liu G, Li X, He B, Tao Y, Guan J, Mu Y, Liu H, Gong W
Cell Discov (2024) 10 pp. 124-124 [ DOI: doi:10.1038/s41421-024-00754-0 Pubmed: 39616153 ]
- Mch-mchr1-gi complex t1 state (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Melanin-concentrating hormone (2 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (27 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Fusion protein 1,melanin-concentrating hormone receptor 1,fusion protein 2 (68 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
MCH-MCHR1-Gi complex, T2 state
Resolution: 2.78 Å
EM Method: Single-particle
Fitted PDBs: 8wwl
Q-score: 0.573
Ye X, Liu G, Li X, He B, Tao Y, Guan J, Mu Y, Liu H, Gong W
Cell Discov (2024) 10 pp. 124-124 [ DOI: doi:10.1038/s41421-024-00754-0 Pubmed: 39616153 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Melanin-concentrating hormone (2 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (27 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Fusion protein 1,melanin-concentrating hormone receptor 1,fusion protein 2 (68 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Mch-mchr1-gi complex t2 state (Complex from Homo sapiens)
MCH-MCHR1-Gi complex, L2 state
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 8wwm
Q-score: 0.531
Ye X, Liu G, Li X, He B, Tao Y, Guan J, Mu Y, Liu H, Gong W
Cell Discov (2024) 10 pp. 124-124 [ DOI: doi:10.1038/s41421-024-00754-0 Pubmed: 39616153 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Mch-mchr1-gi complex l2 state (Complex from Homo sapiens)
- Antibody fragment scfv16 (27 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Melanin-concentrating hormone (2 kDa, Protein from Homo sapiens)
- Fusion protein 1,melanin-concentrating hormone receptor 1,fusion protein 2 (68 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
Resolution: 2.65 Å
EM Method: Single-particle
Fitted PDBs: 8wwn
Q-score: 0.6
Ye X, Liu G, Li X, He B, Tao Y, Guan J, Mu Y, Liu H, Gong W
Cell Discov (2024) 10 pp. 124-124 [ DOI: doi:10.1038/s41421-024-00754-0 Pubmed: 39616153 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Melanin-concentrating hormone (2 kDa, Protein from Homo sapiens)
- Antibody fragment scfv16 (27 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Fusion protein 1,melanin-concentrating hormone receptor 1,fusion protein 2 (68 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Mch-mchr1-gi complex l1 state (Complex from Homo sapiens)
Cryo-EM structure of histamine H3 receptor in complex with immethridine and miniGo
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 8yn7
Q-score: 0.548
Zhang X, Liu G, Zhong YN, Zhang R, Yang CC, Niu C, Pu X, Sun J, Zhang T, Yang L, Zhang C, Li X, Shen X, Xiao P, Sun JP, Gong W
Nat Commun (2024) 15 pp. 8296-8296 [ DOI: doi:10.1038/s41467-024-52585-y Pubmed: 39333117 ]
- 4-(1h-imidazol-4-ylmethyl)pyridine (159 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Engineered guanine nucleotide-binding protein g(o) subunit alpha,guanine nucleotide-binding protein g(o) subunit alpha (25 kDa, Protein from synthetic construct)
- Antibody fragment scfv16 (27 kDa, Protein from synthetic construct)
- Immethridine-h3r-minigo-scfv16 complex (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Histamine h3 receptor (72 kDa, Protein from Homo sapiens)
Cryo-EM structure of histamine H3 receptor in complex with proxyfan and miniGo
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 8yn8
Q-score: 0.544
Zhang X, Liu G, Zhong YN, Zhang R, Yang CC, Niu C, Pu X, Sun J, Zhang T, Yang L, Zhang C, Li X, Shen X, Xiao P, Sun JP, Gong W
Nat Commun (2024) 15 pp. 8296-8296 [ DOI: doi:10.1038/s41467-024-52585-y Pubmed: 39333117 ]
- Antibody fragment scfv16 (27 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- 5-(3-phenylmethoxypropyl)-1h-imidazole (216 Da, Ligand)
- Engineered guanine nucleotide-binding protein g(o) subunit alpha,guanine nucleotide-binding protein g(o) subunit alpha (25 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Histamine h3 receptor (72 kDa, Protein from Homo sapiens)
- Proxyfan-h3r-minigo-scfv16 complex (Complex from Homo sapiens)
Cryo-EM structure of histamine H1 receptor in complex with histamine and miniGq
Resolution: 2.66 Å
EM Method: Single-particle
Fitted PDBs: 8yn2
Q-score: 0.578
Zhang X, Liu G, Zhong YN, Zhang R, Yang CC, Niu C, Pu X, Sun J, Zhang T, Yang L, Zhang C, Li X, Shen X, Xiao P, Sun JP, Gong W
Nat Commun (2024) 15 pp. 8296-8296 [ DOI: doi:10.1038/s41467-024-52585-y Pubmed: 39333117 ]
- Antibody fragment scfv16 (27 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Histamine h1 receptor (80 kDa, Protein from Homo sapiens)
- Engineered guanine nucleotide-binding protein g(q) subunit alpha (28 kDa, Protein from synthetic construct)
- Histamine-h1r-minigq-scfv16 complex (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Histamine (111 Da, Ligand)
Cryo-EM structure of histamine H2 receptor in complex with histamine and miniGs
Resolution: 2.56 Å
EM Method: Single-particle
Fitted PDBs: 8yn3
Q-score: 0.623
Zhang X, Liu G, Zhong YN, Zhang R, Yang CC, Niu C, Pu X, Sun J, Zhang T, Yang L, Zhang C, Li X, Shen X, Xiao P, Sun JP, Gong W
Nat Commun (2024) 15 pp. 8296-8296 [ DOI: doi:10.1038/s41467-024-52585-y Pubmed: 39333117 ]
- Nanobody nb35 (15 kDa, Protein from synthetic construct)
- Engineered guanine nucleotide,binding protein g(s) subunit alpha (29 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Histamine-h2r-minigs-nb35 complex (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Histamine (111 Da, Ligand)
- Histamine h2 receptor (62 kDa, Protein from Homo sapiens)