Cryo-EM reveals cholesterol binding in the lysosomal GPCR-like protein LYCHOS
Zhao J, Shen Q, Yong X, Li X, Tian X, Sun S, Xu Z, Zhang X, Zhang L, Yang H, Shao Z, Xu H, Jiang Y, Zhang Y, Yan W
Nat Struct Mol Biol (2025) [ Pubmed: 39824976 DOI: doi:10.1038/s41594-024-01470-9 ]
- [(2r)-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] octadecanoate (720 Da, Ligand)
- Water (18 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Lysosomal cholesterol signaling protein (93 kDa, Protein from Homo sapiens)
- Cholesterol-bound lychos (Complex from Homo sapiens)
Cryo-EM structure of SARS-CoV-2 Omicron BA.2.86 spike protein(6P), 1-RBD-up state
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 8xlv
Q-score: 0.354
Cryo-EM structure of SARS-CoV-2 Omicron EG.5 spike protein(6P), RBD-closed state
Resolution: 2.61 Å
EM Method: Single-particle
Fitted PDBs: 8xm5
Q-score: 0.446
Cryo-EM structure of SARS-CoV-2 Omicron HV.1 spike protein(6P), RBD-closed state
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8xmg
Q-score: 0.443
Cryo-EM structure of SARS-CoV-2 Omicron EG.5.1 spike protein(6P), RBD-closed state
Resolution: 3.31 Å
EM Method: Single-particle
Fitted PDBs: 8xmt
Q-score: 0.399
SARS-CoV-2 Omicron EG.5.1 RBD in complex with human ACE2 (local refined from the spike protein)
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 8xn2
Q-score: 0.355
Li L, Shi K, Gu Y, Xu Z, Shu C, Li D, Sun J, Cong M, Li X, Zhao X, Yu G, Hu S, Tan H, Qi J, Ma X, Liu K, Gao GF
Structure (2024) 32 pp. 1055-1067.e6 [ Pubmed: 39013463 DOI: doi:10.1016/j.str.2024.06.012 ]
- Sars-cov-2 omicron eg.5.1 rbd (receptor binding domain) in complex with human ace2 (angiotensin converting enzyme 2) (Complex from Severe acute respiratory syndrome coronavirus 2)
- Angiotensin-converting enzyme 2 (69 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Spike protein s1 (25 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
SARS-CoV-2 Omicron HV.1 RBD in complex with human ACE2 (local refinement from the spike protein)
Resolution: 2.64 Å
EM Method: Single-particle
Fitted PDBs: 8xn3
Q-score: 0.355
Li L, Shi K, Gu Y, Xu Z, Shu C, Li D, Sun J, Cong M, Li X, Zhao X, Yu G, Hu S, Tan H, Qi J, Ma X, Liu K, Gao GF
Structure (2024) 32 pp. 1055-1067.e6 [ Pubmed: 39013463 DOI: doi:10.1016/j.str.2024.06.012 ]
- Sars-cov-2 omicron hv.1 rbd (receptor binding domain) in complex with human ace2 (angiotensin converting enzyme 2) (Complex from Severe acute respiratory syndrome coronavirus 2)
- Angiotensin-converting enzyme 2 (69 kDa, Protein from Homo sapiens)
- Spike protein s1 (25 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Zinc ion (65 Da, Ligand)
Cryo-EM structure of SARS-CoV-2 Omicron EG.5.1 spike protein(6P) in complex with human ACE2
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 8xn5
Q-score: 0.371
Li L, Shi K, Gu Y, Xu Z, Shu C, Li D, Sun J, Cong M, Li X, Zhao X, Yu G, Hu S, Tan H, Qi J, Ma X, Liu K, Gao GF
Structure (2024) 32 pp. 1055-1067.e6 [ Pubmed: 39013463 DOI: doi:10.1016/j.str.2024.06.012 ]
- Sars-cov-2 omicron eg.5.1 spike protein(6p) in complex with human ace2 (Complex from Severe acute respiratory syndrome coronavirus 2)
- Angiotensin-converting enzyme 2 (69 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Spike glycoprotein (136 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
Cryo-EM structure of SARS-CoV-2 Omicron BA.2.86 spike protein(6P) in complex with human ACE2
Resolution: 3.26 Å
EM Method: Single-particle
Fitted PDBs: 8xnf
Q-score: 0.317
Li L, Shi K, Gu Y, Xu Z, Shu C, Li D, Sun J, Cong M, Li X, Zhao X, Yu G, Hu S, Tan H, Qi J, Ma X, Liu K, Gao GF
Structure (2024) 32 pp. 1055-1067.e6 [ Pubmed: 39013463 DOI: doi:10.1016/j.str.2024.06.012 ]
- Sars-cov-2 omicron ba.2.86.1 spike protein(6p) in complex with human ace2 (angiotensin converting enzyme 2) (Complex from Severe acute respiratory syndrome coronavirus 2)
- Spike glycoprotein (131 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Angiotensin-converting enzyme 2 (69 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Zinc ion (65 Da, Ligand)
Cryo-EM structure of SARS-CoV-2 Omicron HV.1 spike protein(6P) in complex with human ACE2
Resolution: 2.78 Å
EM Method: Single-particle
Fitted PDBs: 8xnk
Q-score: 0.365
Li L, Shi K, Gu Y, Xu Z, Shu C, Li D, Sun J, Cong M, Li X, Zhao X, Yu G, Hu S, Tan H, Qi J, Ma X, Liu K, Gao GF
Structure (2024) 32 pp. 1055-1067.e6 [ Pubmed: 39013463 DOI: doi:10.1016/j.str.2024.06.012 ]
- Sars-cov-2 omicron hv.1 spike protein(6p) in complex with human ace2 (Complex from Severe acute respiratory syndrome coronavirus 2)
- Angiotensin-converting enzyme 2 (69 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Spike glycoprotein (136 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)