Human PORCN in complex with Palmitoleoyl-CoA
Resolution: 3.11 Å
EM Method: Single-particle
Fitted PDBs: 7ura
Q-score: 0.557
Liu Y, Qi X, Donnelly L, Elghobashi-Meinhardt N, Long T, Zhou RW, Sun Y, Wang B, Li X
Nature (2022) 607 pp. 816-822 [ Pubmed: 35831507 DOI: doi:10.1038/s41586-022-04952-2 ]
- 2c11 heavy chain (26 kDa, Protein from Mus musculus)
- ~{s}-[2-[3-[[(2~{r})-4-[[[(2~{r},3~{r},4~{r},5~{r})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] (~{z})-hexadec-9-enethioate (1 kDa, Ligand)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (52 kDa, Protein from Homo sapiens)
- 2c11 light chain (25 kDa, Protein from Mus musculus)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (e.c.2.3.1.250) (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Oleic acid (282 Da, Ligand)
- 16:1 coa-bound human porcn (Complex)
- 2c11 light chain, 2c11 heavy chain (Complex from Mus musculus)
- Zinc ion (65 Da, Ligand)
- Digitonin (1 kDa, Ligand)
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 7urc
Q-score: 0.532
Liu Y, Qi X, Donnelly L, Elghobashi-Meinhardt N, Long T, Zhou RW, Sun Y, Wang B, Li X
Nature (2022) 607 pp. 816-822 [ Pubmed: 35831507 DOI: doi:10.1038/s41586-022-04952-2 ]
- 2c11 heavy chain (26 kDa, Protein from Mus musculus)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (52 kDa, Protein from Homo sapiens)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (e.c.2.3.1.250) (Complex from Homo sapiens)
- 2c11 light chain (25 kDa, Protein from Mus musculus)
- Cholesterol (386 Da, Ligand)
- 2-[(2p)-2',3-dimethyl[2,4'-bipyridin]-5-yl]-n-[(5p)-5-(pyrazin-2-yl)pyridin-2-yl]acetamide (396 Da, Ligand)
- Digitonin (1 kDa, Ligand)
- 2c11 light chain, 2c11 heavy chain (Complex from Mus musculus)
- Zinc ion (65 Da, Ligand)
- Lgk974-bound human porcn (Complex)
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 7urd
Q-score: 0.569
Liu Y, Qi X, Donnelly L, Elghobashi-Meinhardt N, Long T, Zhou RW, Sun Y, Wang B, Li X
Nature (2022) 607 pp. 816-822 [ Pubmed: 35831507 DOI: doi:10.1038/s41586-022-04952-2 ]
- Isoform 2 of protein wnt-3a peptide (2 kDa, Protein from Homo sapiens)
- 2c11 heavy chain (26 kDa, Protein from Mus musculus)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (52 kDa, Protein from Homo sapiens)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (e.c.2.3.1.250) (Complex from Homo sapiens)
- Lgk974 and wnt3a peptide-bound human porcn (Complex)
- 2c11 light chain (25 kDa, Protein from Mus musculus)
- Cholesterol (386 Da, Ligand)
- Oleic acid (282 Da, Ligand)
- Isoform 2 of protein wnt-3a peptide (Complex)
- 2-[(2p)-2',3-dimethyl[2,4'-bipyridin]-5-yl]-n-[(5p)-5-(pyrazin-2-yl)pyridin-2-yl]acetamide (396 Da, Ligand)
- 2c11 light chain, 2c11 heavy chain (Complex from Mus musculus)
- Zinc ion (65 Da, Ligand)
- Digitonin (1 kDa, Ligand)
Human PORCN in complex with palmitoleoylated WNT3A peptide
Resolution: 3.19 Å
EM Method: Single-particle
Fitted PDBs: 7ure
Q-score: 0.539
Liu Y, Qi X, Donnelly L, Elghobashi-Meinhardt N, Long T, Zhou RW, Sun Y, Wang B, Li X
Nature (2022) 607 pp. 816-822 [ Pubmed: 35831507 DOI: doi:10.1038/s41586-022-04952-2 ]
- Protein wnt-3a (2 kDa, Protein from Homo sapiens)
- Palmitoleic acid (254 Da, Ligand)
- 2c11 heavy chain (26 kDa, Protein from Mus musculus)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (52 kDa, Protein from Homo sapiens)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (e.c.2.3.1.250) (Complex from Homo sapiens)
- 2c11 light chain (25 kDa, Protein from Mus musculus)
- Isoform 2 of protein wnt-3a (Complex)
- Product-bound human porcn (Complex)
- 2c11 light chain, 2c11 heavy chain (Complex from Mus musculus)
- Zinc ion (65 Da, Ligand)
- Digitonin (1 kDa, Ligand)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7urf
Q-score: 0.597
Liu Y, Qi X, Donnelly L, Elghobashi-Meinhardt N, Long T, Zhou RW, Sun Y, Wang B, Li X
Nature (2022) 607 pp. 816-822 [ Pubmed: 35831507 DOI: doi:10.1038/s41586-022-04952-2 ]
- 3h02 heavy chain, 3h02 light chain (Complex from Mus musculus)
- 3h02 heavy chain (27 kDa, Protein from Mus musculus)
- 3h02 light chain (26 kDa, Protein from Mus musculus)
- 16:0 coa and shh-n-bound human hhat (Complex)
- Protein-cysteine n-palmitoyltransferase hhat (e.c.2.3.1.-), shh-n peptide (Complex from Homo sapiens)
- Protein-cysteine n-palmitoyltransferase hhat (58 kDa, Protein from Homo sapiens)
- Shh-n peptide (693 Da, Protein from Homo sapiens)
- Digitonin (1 kDa, Ligand)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Palmitoyl-coa (1 kDa, Ligand)
Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 7n4u
Q-score: 0.467
Long T, Liu Y, Qin Y, DeBose-Boyd RA, Li X
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abh3997 Pubmed: 34407950 ]
- (2r)-2,5,7,8-tetramethyl-2-[(4r,8r)-4,8,12-trimethyltridecyl]chroman-6-ol (430 Da, Ligand)
- Isoform 2 of npc1-like intracellular cholesterol transporter 1 (145 kDa, Protein from Homo sapiens)
- Npc1l1 apo state (Cell from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
Structure of cholesterol-bound human NPC1L1
Resolution: 3.58 Å
EM Method: Single-particle
Fitted PDBs: 7n4v
Q-score: 0.453
Long T, Liu Y, Qin Y, DeBose-Boyd RA, Li X
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abh3997 Pubmed: 34407950 ]
- Isoform 2 of npc1-like intracellular cholesterol transporter 1 (145 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Human npc1l1 supplemented with cholesterol (Cellular component from Homo sapiens)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
Structure of human NPC1L1 mutant-W347R
Resolution: 3.33 Å
EM Method: Single-particle
Fitted PDBs: 7n4x
Q-score: 0.525
Long T, Liu Y, Qin Y, DeBose-Boyd RA, Li X
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abh3997 Pubmed: 34407950 ]
- Isoform 2 of npc1-like intracellular cholesterol transporter 1 (145 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Human npc1l1 mutant-w347r (Cellular component from Homo sapiens)
Structure of Human Dispatched-1 (DISP1)
Resolution: 4.53 Å
EM Method: Single-particle
Fitted PDBs: 6xe6
Q-score: 0.33
Life Sci Alliance (2020) 3 [ DOI: doi:10.26508/lsa.202000776 Pubmed: 32646883 ]
- Human dispatched-1 in complex with dual-lipidated shh-n (Cellular component from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Protein dispatched homolog 1 (124 kDa, Protein from Homo sapiens)