Human PORCN in complex with Palmitoleoyl-CoA
Resolution: 3.11 Å
EM Method: Single-particle
Fitted PDBs: 7ura
Q-score: 0.557
Liu Y, Qi X, Donnelly L, Elghobashi-Meinhardt N, Long T, Zhou RW, Sun Y, Wang B, Li X
Nature (2022) 607 pp. 816-822 [ Pubmed: 35831507 DOI: doi:10.1038/s41586-022-04952-2 ]
- Cholesterol (386 Da, Ligand)
- 2c11 light chain, 2c11 heavy chain (Complex from Mus musculus)
- Digitonin (1 kDa, Ligand)
- Zinc ion (65 Da, Ligand)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (52 kDa, Protein from Homo sapiens)
- 2c11 heavy chain (26 kDa, Protein from Mus musculus)
- 2c11 light chain (25 kDa, Protein from Mus musculus)
- ~{s}-[2-[3-[[(2~{r})-4-[[[(2~{r},3~{r},4~{r},5~{r})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] (~{z})-hexadec-9-enethioate (1 kDa, Ligand)
- 16:1 coa-bound human porcn (Complex)
- Oleic acid (282 Da, Ligand)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (e.c.2.3.1.250) (Complex from Homo sapiens)
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 7urc
Q-score: 0.532
Liu Y, Qi X, Donnelly L, Elghobashi-Meinhardt N, Long T, Zhou RW, Sun Y, Wang B, Li X
Nature (2022) 607 pp. 816-822 [ Pubmed: 35831507 DOI: doi:10.1038/s41586-022-04952-2 ]
- 2c11 heavy chain (26 kDa, Protein from Mus musculus)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (52 kDa, Protein from Homo sapiens)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (e.c.2.3.1.250) (Complex from Homo sapiens)
- 2c11 light chain (25 kDa, Protein from Mus musculus)
- Cholesterol (386 Da, Ligand)
- 2-[(2p)-2',3-dimethyl[2,4'-bipyridin]-5-yl]-n-[(5p)-5-(pyrazin-2-yl)pyridin-2-yl]acetamide (396 Da, Ligand)
- Digitonin (1 kDa, Ligand)
- 2c11 light chain, 2c11 heavy chain (Complex from Mus musculus)
- Zinc ion (65 Da, Ligand)
- Lgk974-bound human porcn (Complex)
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 7urd
Q-score: 0.569
Liu Y, Qi X, Donnelly L, Elghobashi-Meinhardt N, Long T, Zhou RW, Sun Y, Wang B, Li X
Nature (2022) 607 pp. 816-822 [ Pubmed: 35831507 DOI: doi:10.1038/s41586-022-04952-2 ]
- Isoform 2 of protein wnt-3a peptide (2 kDa, Protein from Homo sapiens)
- 2c11 heavy chain (26 kDa, Protein from Mus musculus)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (52 kDa, Protein from Homo sapiens)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (e.c.2.3.1.250) (Complex from Homo sapiens)
- Lgk974 and wnt3a peptide-bound human porcn (Complex)
- 2c11 light chain (25 kDa, Protein from Mus musculus)
- Cholesterol (386 Da, Ligand)
- Oleic acid (282 Da, Ligand)
- Isoform 2 of protein wnt-3a peptide (Complex)
- 2-[(2p)-2',3-dimethyl[2,4'-bipyridin]-5-yl]-n-[(5p)-5-(pyrazin-2-yl)pyridin-2-yl]acetamide (396 Da, Ligand)
- 2c11 light chain, 2c11 heavy chain (Complex from Mus musculus)
- Zinc ion (65 Da, Ligand)
- Digitonin (1 kDa, Ligand)
Human PORCN in complex with palmitoleoylated WNT3A peptide
Resolution: 3.19 Å
EM Method: Single-particle
Fitted PDBs: 7ure
Q-score: 0.539
Liu Y, Qi X, Donnelly L, Elghobashi-Meinhardt N, Long T, Zhou RW, Sun Y, Wang B, Li X
Nature (2022) 607 pp. 816-822 [ Pubmed: 35831507 DOI: doi:10.1038/s41586-022-04952-2 ]
- Protein wnt-3a (2 kDa, Protein from Homo sapiens)
- Palmitoleic acid (254 Da, Ligand)
- 2c11 heavy chain (26 kDa, Protein from Mus musculus)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (52 kDa, Protein from Homo sapiens)
- Isoform 2 of protein-serine o-palmitoleoyltransferase porcupine (e.c.2.3.1.250) (Complex from Homo sapiens)
- 2c11 light chain (25 kDa, Protein from Mus musculus)
- Isoform 2 of protein wnt-3a (Complex)
- Product-bound human porcn (Complex)
- 2c11 light chain, 2c11 heavy chain (Complex from Mus musculus)
- Zinc ion (65 Da, Ligand)
- Digitonin (1 kDa, Ligand)
Structure of itraconazole-bound NPC1
Resolution: 4.02 Å
EM Method: Single-particle
Fitted PDBs: 6uox
Q-score: 0.372
Long T, Qi X, Hassan A, Liang Q, De Brabander JK, Li X
Nat Commun (2020) 11 pp. 152-152 [ DOI: doi:10.1038/s41467-019-13917-5 Pubmed: 31919352 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 4-(3-bromo-4-{4-[4-({(2r,4s)-2-(2,4-dichlorophenyl)-2-[(1h-1,2,4-triazol-1-yl)methyl]-1,3-dioxolan-4-yl}methoxy)phenyl]piperazin-1-yl}phenyl)-2-[(2s)-butan-2-yl]-2,4-dihydro-3h-1,2,4-triazol-3-one (784 Da, Ligand)
- Npc intracellular cholesterol transporter 1 (143 kDa, Protein from Homo sapiens)
- Npc1 and itraconazole-br complex (Complex from Homo sapiens)