Cryo-EM Structure of MILI(K852A)-piRNA-target (26-nt)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 9ij4
Q-score: 0.474
Li Z, Xu Q, Zhong J, Zhang Y, Zhang T, Ying X, Lu X, Li X, Wan L, Xue J, Huang J, Zhen Y, Zhang Z, Wu J, Shen EZ
Nature (2025) [ DOI: doi:10.1038/s41586-024-08438-1 Pubmed: 39814893 ]
- Rna (25-mer) (8 kDa, RNA from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Mili(k852a)-pirna-target (26-nt) (Complex from Homo sapiens)
- Rna (26-mer) (8 kDa, RNA from Homo sapiens)
- Piwi-like protein 2 (109 kDa, Protein from Mus musculus)
Curved structure of mPIEZO1-S2472E
Resolution: 4.07 Å
EM Method: Single-particle
Fitted PDBs: 8ixn
Q-score: 0.301
Liu S, Yang X, Chen X, Zhang X, Jiang J, Yuan J, Liu W, Wang L, Zhou H, Wu K, Tian B, Li X, Xiao B
Neuron (2024) [ Pubmed: 39719701 DOI: doi:10.1016/j.neuron.2024.11.020 ]
- Piezo-type mechanosensitive ion channel component 1 (292 kDa, Protein from Mus musculus)
- (9r,11s)-9-({[(1s)-1-hydroxyhexadecyl]oxy}methyl)-2,2-dimethyl-5,7,10-trioxa-2lambda~5~-aza-6lambda~5~-phosphaoctacosane-6,6,11-triol (767 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- S2472e-curved structure (Complex from Mus musculus)
- O-[(r)-{[(2r)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-l-serine (792 Da, Ligand)
Flattened structure of mPIEZO1-S2472E
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 8ixo
Q-score: 0.445
Liu S, Yang X, Chen X, Zhang X, Jiang J, Yuan J, Liu W, Wang L, Zhou H, Wu K, Tian B, Li X, Xiao B
Neuron (2024) [ Pubmed: 39719701 DOI: doi:10.1016/j.neuron.2024.11.020 ]
- Piezo-type mechanosensitive ion channel component 1 (292 kDa, Protein from Mus musculus)
- S2472e-intermediate structure (Complex from Mus musculus)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- (9r,11s)-9-({[(1s)-1-hydroxyhexadecyl]oxy}methyl)-2,2-dimethyl-5,7,10-trioxa-2lambda~5~-aza-6lambda~5~-phosphaoctacosane-6,6,11-triol (767 Da, Ligand)
- O-[(r)-{[(2r)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-l-serine (792 Da, Ligand)
Cryo-EM Structure of MILI-piRNA-target (8-nt)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 9ij0
Q-score: 0.417
Li Z, Xu Q, Zhong J, Zhang Y, Zhang T, Ying X, Lu X, Li X, Wan L, Xue J, Huang J, Zhen Y, Zhang Z, Wu J, Shen EZ
Nature (2025) [ DOI: doi:10.1038/s41586-024-08438-1 Pubmed: 39814893 ]
- Rna (5'-r(p*gp*ap*up*gp*gp*up*ap*a)-3') (2 kDa, RNA from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Piwi-like protein 2 (109 kDa, Protein from Mus musculus)
- Mili-pirna-target (8-nt) (Complex from Homo sapiens)
- Rna (26-mer) (8 kDa, RNA from Homo sapiens)
Cryo-EM Structure of MILI-piRNA-target (22-nt, bilobed)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 9ij1
Q-score: 0.325
Li Z, Xu Q, Zhong J, Zhang Y, Zhang T, Ying X, Lu X, Li X, Wan L, Xue J, Huang J, Zhen Y, Zhang Z, Wu J, Shen EZ
Nature (2025) [ DOI: doi:10.1038/s41586-024-08438-1 Pubmed: 39814893 ]
- Rna (5'-r(p*cp*ap*ap*gp*up*up*up*cp*cp*ap*up*gp*up*up*gp*ap*up*gp*gp*up*a)-3') (6 kDa, RNA from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Piwi-like protein 2 (109 kDa, Protein from Mus musculus)
- Mili-pirna-target (22-nt, bilobed) (Complex from Homo sapiens)
- Rna (26-mer) (8 kDa, RNA from Homo sapiens)
Cryo-EM Structure of MILI-piRNA-target (22-nt, comma)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 9ij2
Q-score: 0.188
Li Z, Xu Q, Zhong J, Zhang Y, Zhang T, Ying X, Lu X, Li X, Wan L, Xue J, Huang J, Zhen Y, Zhang Z, Wu J, Shen EZ
Nature (2025) [ DOI: doi:10.1038/s41586-024-08438-1 Pubmed: 39814893 ]
- Rna (5'-r(p*up*up*ap*cp*cp*ap*up*cp*ap*ap*cp*ap*up*gp*gp*ap*ap*ap*cp*up*up*g)-3') (6 kDa, RNA from Homo sapiens)
- Mili-pirna-target (22-nt, comma) (Complex from Homo sapiens)
- Rna (5'-r(p*cp*ap*ap*gp*up*up*up*cp*cp*ap*up*gp*up*up*gp*ap*up*gp*gp*up*a)-3') (6 kDa, RNA from Homo sapiens)
- Piwi-like protein 2 (109 kDa, Protein from Mus musculus)
Cryo-EM Structure of MILI-piRNA-target (26-nt)
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 9ij3
Q-score: 0.463
Li Z, Xu Q, Zhong J, Zhang Y, Zhang T, Ying X, Lu X, Li X, Wan L, Xue J, Huang J, Zhen Y, Zhang Z, Wu J, Shen EZ
Nature (2025) [ DOI: doi:10.1038/s41586-024-08438-1 Pubmed: 39814893 ]
- Mili-pirna-target (26-nt) (Complex from Homo sapiens)
- Rna (26-mer) (8 kDa, RNA from Homo sapiens)
- Piwi-like protein 2 (109 kDa, Protein from Mus musculus)
- Rna (25-mer) (8 kDa, RNA from Homo sapiens)
- Manganese (ii) ion (54 Da, Ligand)
Cryo-EM Structure of MILI(K853A)-piRNA-target
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 9ij5
Q-score: 0.461
Li Z, Xu Q, Zhong J, Zhang Y, Zhang T, Ying X, Lu X, Li X, Wan L, Xue J, Huang J, Zhen Y, Zhang Z, Wu J, Shen EZ
Nature (2025) [ DOI: doi:10.1038/s41586-024-08438-1 Pubmed: 39814893 ]
- Rna (26-mer) (8 kDa, RNA from Homo sapiens)
- Piwi-like protein 2 (109 kDa, Protein from Mus musculus)
- Rna (25-mer) (8 kDa, RNA from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Mili(k853a)-pirna-target (Complex from Homo sapiens)