Cryo-EM structure of the PAC1R-PACAP27-Gs complex
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 8e3x
Q-score: 0.543
Piper SJ, Deganutti G, Lu J, Zhao P, Liang YL, Lu Y, Fletcher MM, Hossain MA, Christopoulos A, Reynolds CA, Danev R, Sexton PM, Wootten D
Nat Commun (2022) 13 pp. 7013-7013 [ Pubmed: 36385145 DOI: doi:10.1038/s41467-022-34629-3 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Gs coupled pituitary adenylate cyclase-activating polypeptide type i receptor complex with pacap27 peptide (Complex)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, pituitary adenylate cyclase-activating polypeptide type i receptor (Complex from Homo sapiens)
- Pituitary adenylate cyclase-activating polypeptide type i receptor (55 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Pituitary adenylate cyclase activating polypeptide-27 (Complex from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Pituitary adenylate cyclase-activating polypeptide (3 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Water (18 Da, Ligand)
Cryo-EM structure of the VPAC1R-PACAP27-Gs complex
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 8e3y
Q-score: 0.576
Piper SJ, Deganutti G, Lu J, Zhao P, Liang YL, Lu Y, Fletcher MM, Hossain MA, Christopoulos A, Reynolds CA, Danev R, Sexton PM, Wootten D
Nat Commun (2022) 13 pp. 7013-7013 [ Pubmed: 36385145 DOI: doi:10.1038/s41467-022-34629-3 ]
- Vasoactive intestinal polypeptide receptor 1 (52 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, vasoactive intestinal polypeptide receptor 1 (Complex from Homo sapiens)
- Pituitary adenylate cyclase activating polypeptide-27 (Complex from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Gs coupled vasoactive intestinal polypeptide receptor 1 (vpac1) complex with pacap27 peptide (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Pituitary adenylate cyclase-activating polypeptide (3 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
Cryo-EM structure of the VPAC1R-VIP-Gs complex
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8e3z
Q-score: 0.483
Piper SJ, Deganutti G, Lu J, Zhao P, Liang YL, Lu Y, Fletcher MM, Hossain MA, Christopoulos A, Reynolds CA, Danev R, Sexton PM, Wootten D
Nat Commun (2022) 13 pp. 7013-7013 [ Pubmed: 36385145 DOI: doi:10.1038/s41467-022-34629-3 ]
- Vasoactive intestinal polypeptide receptor 1 (52 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, vasoactive intestinal polypeptide receptor 1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Gs coupled vasoactive intestinal polypeptide receptor 1 (vpac1) complex with vip peptide (Complex)
- Vasoactive intestinal peptide (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Vasoactive intestinal peptide-vip (Complex from Homo sapiens)
- Water (18 Da, Ligand)
CryoEM structure of the apo-CGRP receptor in a detergent micelle
Resolution: 3.15 Å
EM Method: Single-particle
Fitted PDBs: 7knt
Q-score: 0.39
Josephs TM, Belousoff MJ, Liang YL, Piper SJ, Cao J, Garama DJ, Leach K, Gregory KJ, Christopoulos A, Hay DL, Danev R, Wootten D, Sexton PM
Science (2021) 372 [ DOI: doi:10.1126/science.abf7258 Pubmed: 33602864 ]
- Apo cgrp receptor (Complex from Homo sapiens)
- Receptor activity-modifying protein 1 (17 kDa, Protein from Homo sapiens)
- Calcitonin gene-related peptide type 1 receptor (56 kDa, Protein from Homo sapiens)
CryoEM structure of the CGRP receptor with bound CGRP peptide in a detergent micelle
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 7knu
Q-score: 0.351
Josephs TM, Belousoff MJ, Liang YL, Piper SJ, Cao J, Garama DJ, Leach K, Gregory KJ, Christopoulos A, Hay DL, Danev R, Wootten D, Sexton PM
Science (2021) 372 [ DOI: doi:10.1126/science.abf7258 Pubmed: 33602864 ]
- Calcitonin gene-related peptide 1 (Complex from Homo sapiens)
- Cgrp receptor bound to cgrp and ramp1 (Complex)
- Calcitonin gene-related peptide 1 (3 kDa, Protein from Homo sapiens)
- Cgrp receptor with ramp1 (Complex from Homo sapiens)
- Receptor activity-modifying protein 1 (17 kDa, Protein from Homo sapiens)
- Calcitonin gene-related peptide type 1 receptor (56 kDa, Protein from Homo sapiens)
Human secretin receptor Gs complex
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6wi9
Q-score: 0.359
Dong M, Deganutti G, Piper SJ, Liang YL, Khoshouei M, Belousoff MJ, Harikumar KG, Reynolds CA, Glukhova A, Furness SGB, Christopoulos A, Danev R, Wootten D, Sexton PM, Miller LJ
Nat Commun (2020) 11 pp. 4137-4137 [ DOI: doi:10.1038/s41467-020-17791-4 Pubmed: 32811827 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Secretin receptor (51 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, secretin receptor (Complex from Homo sapiens)
- Gs coupled sectretin complex with secretin peptide (Complex)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Secretin (3 kDa, Protein from Homo sapiens)
- Secretin (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
Human secretin receptor Gs complex
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 6wzg
Q-score: 0.609
Dong M, Deganutti G, Piper SJ, Liang YL, Khoshouei M, Belousoff MJ, Harikumar KG, Reynolds CA, Glukhova A, Furness SGB, Christopoulos A, Danev R, Wootten D, Sexton PM, Miller LJ
Nat Commun (2020) 11 pp. 4137-4137 [ DOI: doi:10.1038/s41467-020-17791-4 Pubmed: 32811827 ]
- Gs coupled secretin complex with secretin peptide (Complex)
- Secretin receptor (51 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, secretin receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Secretin (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Secretin (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)