CryoEM of PreP prepared via Chameleon
Liang WG, Wijaya J, Wei H, Noble AJ, Mancl JM, Mo S, Lee D, Lin King JV, Pan M, Liu C, Koehler CM, Zhao M, Potter CS, Carragher B, Li S, Tang WJ
Nat Commun (2022) 13 [ Pubmed: 35383169 DOI: 10.1038/s41467-022-29322-4 ]
Image sets:
- Citrate synthase presequence bound PreP
- PreP half particles
- Amyloid beta bound PreP
- Apo-PreP micrographs
CryoET of presequence protease single particle
Liang WG, Wijaya J, Wei H, Noble AJ, Mancl JM, Mo S, Lee D, Lin King JV, Pan M, Liu C, Koehler CM, Zhao M, Potter CS, Carragher B, Li S, Tang WJ
Nat Commun (2022) 13 [ Pubmed: 35383169 DOI: 10.1038/s41467-022-29322-4 ]
Image sets:
CryoET of presequence protease single particle
Liang WG, Wijaya J, Wei H, Noble AJ, Mancl JM, Mo S, Lee D, Lin King JV, Pan M, Liu C, Koehler CM, Zhao M, Potter CS, Carragher B, Li S, Tang WJ
Nat Commun (2022) 13 pp. 1833-1833 [ DOI: doi:10.1038/s41467-022-29322-4 Pubmed: 35383169 ]
- Human presequence protease (117 kDa, Complex from Escherichia phage EcSzw-2)
CryoEM structure of human presequence protease in partial open state 1
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6xos
Q-score: 0.405
Liang WG, Wijaya J, Wei H, Noble AJ, Mancl JM, Mo S, Lee D, Lin King JV, Pan M, Liu C, Koehler CM, Zhao M, Potter CS, Carragher B, Li S, Tang WJ
Nat Commun (2022) 13 pp. 1833-1833 [ DOI: doi:10.1038/s41467-022-29322-4 Pubmed: 35383169 ]
CryoEM structure of human presequence protease in partial open state 2
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6xot
Q-score: 0.315
Liang WG, Wijaya J, Wei H, Noble AJ, Mancl JM, Mo S, Lee D, Lin King JV, Pan M, Liu C, Koehler CM, Zhao M, Potter CS, Carragher B, Li S, Tang WJ
Nat Commun (2022) 13 pp. 1833-1833 [ DOI: doi:10.1038/s41467-022-29322-4 Pubmed: 35383169 ]
CryoEM structure of human presequence protease in open state
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6xou
Q-score: 0.333
Liang WG, Wijaya J, Wei H, Noble AJ, Mancl JM, Mo S, Lee D, Lin King JV, Pan M, Liu C, Koehler CM, Zhao M, Potter CS, Carragher B, Li S, Tang WJ
Nat Commun (2022) 13 pp. 1833-1833 [ DOI: doi:10.1038/s41467-022-29322-4 Pubmed: 35383169 ]
CryoEM structure of human presequence protease in partial closed state 1
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6xov
Q-score: 0.468
Liang WG, Wijaya J, Wei H, Noble AJ, Mancl JM, Mo S, Lee D, Lin King JV, Pan M, Liu C, Koehler CM, Zhao M, Potter CS, Carragher B, Li S, Tang WJ
Nat Commun (2022) 13 pp. 1833-1833 [ DOI: doi:10.1038/s41467-022-29322-4 Pubmed: 35383169 ]
- Presequence protease, mitochondrial (114 kDa, Protein from Homo sapiens)
- Cryoem map of human presequence protease in partial close state 1 (Complex from Homo sapiens)
- Amyloid-beta precursor protein (273 Da, Protein from Homo sapiens)
- Amyloid-beta precursor protein (4 kDa, Protein from Homo sapiens)
Structure of TRPA1 modified by Bodipy-iodoacetamide with bound calcium, LMNG
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 6v9v
Q-score: 0.509
Zhao J, Lin King JV, Paulsen CE, Cheng Y, Julius D
Nature (2020) 585 pp. 141-145 [ Pubmed: 32641835 DOI: doi:10.1038/s41586-020-2480-9 ]
- ~{n}-[[2,2-bis(fluoranyl)-10,12-dimethyl-1,3-diaza-2$l^{4}-boratricyclo[7.3.0.0^{3,7}]dodeca-4,6,9,11-tetraen-4-yl]methyl]ethanamide (292 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Transient receptor potential cation channel subfamily a member 1 (127 kDa, Protein from Homo sapiens)
- Trpa1 modified by bodipy-iodoacetamide with bound calcium, solubilized in lmng (Complex from Homo sapiens)
Structure of TRPA1 (ligand-free) with bound calcium, LMNG
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6v9w
Q-score: 0.419
Zhao J, Lin King JV, Paulsen CE, Cheng Y, Julius D
Nature (2020) 585 pp. 141-145 [ Pubmed: 32641835 DOI: doi:10.1038/s41586-020-2480-9 ]
Structure of TRPA1 modified by iodoacetamide, PMAL-C8
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6v9x
Q-score: 0.475
Zhao J, Lin King JV, Paulsen CE, Cheng Y, Julius D
Nature (2020) 585 pp. 141-145 [ Pubmed: 32641835 DOI: doi:10.1038/s41586-020-2480-9 ]