Cryo-EM structure of LbCas12a-crRNA: AcrVA4 (1:2 complex)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6p7m
Q-score: 0.549
Knott GJ, Cress BF, Liu JJ, Thornton BW, Lew RJ, Al-Shayeb B, Rosenberg DJ, Hammel M, Adler BA, Lobba MJ, Xu M, Arkin AP, Fellmann C, Doudna JA
eLife (2019) 8 [ DOI: doi:10.7554/eLife.49110 Pubmed: 31397669 ]
- Mature crrna (12 kDa, RNA from Lachnospiraceae bacterium ND2006)
- Lbcas12a-crrna-acrva4 complex (1:2, state i) (Complex)
- Anti-crispr va4 (27 kDa, Protein from Moraxella bovoculi)
- Cas12a (144 kDa, Protein from Lachnospiraceae bacterium ND2006)
- Crrna (Complex from Lachnospiraceae bacterium ND2006)
- Acrva4 (Complex from Moraxella bovoculi)
- Magnesium ion (24 Da, Ligand)
- Lbcas12a (Complex from Lachnospiraceae bacterium ND2006)
Cryo-EM structure of LbCas12a-crRNA: AcrVA4 (2:2 complex)
Resolution: 4.9 Å
EM Method: Single-particle
Fitted PDBs: 6p7n
Q-score: 0.158
Knott GJ, Cress BF, Liu JJ, Thornton BW, Lew RJ, Al-Shayeb B, Rosenberg DJ, Hammel M, Adler BA, Lobba MJ, Xu M, Arkin AP, Fellmann C, Doudna JA
eLife (2019) 8 [ DOI: doi:10.7554/eLife.49110 Pubmed: 31397669 ]
- Mature crrna (12 kDa, RNA from Lachnospiraceae bacterium ND2006)
- Lbcas12a-crrna-acrva4 complex (2:2, state i) (Complex)
- Anti-crispr va4 (27 kDa, Protein from Moraxella bovoculi)
- Crrna (Complex from Lachnospiraceae bacterium ND2006)
- Cas12a (144 kDa, Protein from Lachnospiraceae bacterium ND2006)
- Acrva4 (Complex from Moraxella bovoculi)
- Magnesium ion (24 Da, Ligand)
- Lbcas12a (Complex from Lachnospiraceae bacterium ND2006)
Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Jonathan C, Tan D, Knott G, Wanger A, Harrington L, Nogales E, Doudna JA
Nature (2019) 566 pp. 218-223 [ ]
- Apocasx (Complex from Deltaproteobacteria)
Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Jonathan C, Tan D, Knott G, Wanger A, Harrington L, Nogales E, Doudna JA
Nature (2019) 566 pp. 218-223 [ ]
CasX-gRNA-DNA (45T-20NT) State I
Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Jonathan C, Tan D, Knott G, Wanger A, Harrington L, Nogales E, Doudna JA
Nature (2019) 566 pp. 218-223 [ ]
CasX-gRNA-DNA (45T-20NT) State II
Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Jonathan C, Tan D, Knott G, Wanger A, Harrington L, Nogales E, Doudna JA
Nature (2019) 566 pp. 218-223 [ ]
CasX-gRNA-DNA (45T-45NT) State II
Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Jonathan C, Tan D, Knott G, Wanger A, Harrington L, Nogales E, Doudna JA
Nature (2019) 566 pp. 218-223 [ ]
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 6ny1
Q-score: 0.368
Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Jonathan C, Tan D, Knott G, Wanger A, Harrington L, Nogales E, Doudna JA
Nature (2019) 566 pp. 218-223 [ ]
CasX ternary complex with 30bp target DNA
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6ny3
Q-score: 0.465
Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brotzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E, Doudna JA
Nature (2019) 566 pp. 218-223 [ Pubmed: 30718774 DOI: doi:10.1038/s41586-019-0908-x ]
- Dna (30-mer) (9 kDa, DNA from Deltaproteobacteria bacterium)
- Dna (24-mer) (7 kDa, DNA from Deltaproteobacteria bacterium)
- Casx ternary complex with 30bp target dna (Complex from Deltaproteobacteria)
- Rna (122-mer) (39 kDa, RNA from Deltaproteobacteria bacterium)
- Casx (113 kDa, Protein from Deltaproteobacteria bacterium)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6ny2
Q-score: 0.472
Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brotzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E, Doudna JA
Nature (2019) 566 pp. 218-223 [ Pubmed: 30718774 DOI: doi:10.1038/s41586-019-0908-x ]
- Dna (35-mer) (10 kDa, DNA from Deltaproteobacteria)
- Casx (113 kDa, Protein from Deltaproteobacteria bacterium RIFCSPLOWO2 12 FULL 43 16)
- Dna (35-mer) (10 kDa, DNA from Deltaproteobacteria)
- Rna (112-mer) (35 kDa, RNA from Deltaproteobacteria)
- Casx-grna-dna(45bp) state i (Complex from Deltaproteobacteria)