Cryo-EM structure of the yeast SPT-ORM2 (ORM2-S3A) complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8iaj
Q-score: 0.567
Xie T, Dong F, Han G, Wu X, Liu P, Zhang Z, Zhong J, Niranjanakumari S, Gable K, Gupta SD, Liu W, Harrison PJ, Campopiano DJ, Dunn TM, Gong X
Cell Rep (2024) 43 pp. 113717-113717 [ Pubmed: 38285738 DOI: doi:10.1016/j.celrep.2024.113717 ]
- Serine palmitoyltransferase-regulating protein tsc3 (9 kDa, Protein from Saccharomyces cerevisiae)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Protein orm2 (24 kDa, Protein from Saccharomyces cerevisiae S288C)
- N-[(2s,3r,4e)-1,3-dihydroxyoctadec-4-en-2-yl]tetracosanamide (650 Da, Ligand)
- Spt-orm2 complex (Complex from Saccharomyces cerevisiae)
- Serine palmitoyltransferase 2 (63 kDa, Protein from Saccharomyces cerevisiae)
- Chimera of long chain base biosynthesis protein 1 and serine palmitoyltransferase 1 (59 kDa, Protein from Saccharomyces cerevisiae S288C )
Cryo-EM structure of the yeast SPT-ORM2 (ORM2-S3A-N71A) complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8iak
Q-score: 0.548
Xie T, Dong F, Han G, Wu X, Liu P, Zhang Z, Zhong J, Niranjanakumari S, Gable K, Gupta SD, Liu W, Harrison PJ, Campopiano DJ, Dunn TM, Gong X
Cell Rep (2024) 43 pp. 113717-113717 [ Pubmed: 38285738 DOI: doi:10.1016/j.celrep.2024.113717 ]
- Protein orm2 (24 kDa, Protein from Saccharomyces cerevisiae)
- Serine palmitoyltransferase-regulating protein tsc3 (9 kDa, Protein from Saccharomyces cerevisiae)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Chimera of long chain base biosynthesis protein 1 and serine palmitoyltransferase 1 (59 kDa, Protein from Saccharomyces cerevisiae S288C)
- Spt-orm2 complex (Complex from Saccharomyces cerevisiae)
- Serine palmitoyltransferase 2 (63 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of the yeast SPT-ORM2 (ORM2-S3D) complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8iam
Q-score: 0.556
Xie T, Dong F, Han G, Wu X, Liu P, Zhang Z, Zhong J, Niranjanakumari S, Gable K, Gupta SD, Liu W, Harrison PJ, Campopiano DJ, Dunn TM, Gong X
Cell Rep (2024) 43 pp. 113717-113717 [ Pubmed: 38285738 DOI: doi:10.1016/j.celrep.2024.113717 ]
- Chimera of long chain base biosynthesis protein 1 and serine palmitoyltransferase 1 (59 kDa, Protein from Saccharomyces cerevisiae)
- Serine palmitoyltransferase-regulating protein tsc3 (9 kDa, Protein from Saccharomyces cerevisiae)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- N-[(2s,3r,4e)-1,3-dihydroxyoctadec-4-en-2-yl]tetracosanamide (650 Da, Ligand)
- Serine palmitoyltransferase 2 (63 kDa, Protein from Saccharomyces cerevisiae)
- Spt-orm2 complex (Complex from Saccharomyces cerevisiae)
- Protein orm2 (24 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of the dimeric atSPT-ORM1 complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7yjk
Q-score: 0.556
Liu P, Xie T, Wu X, Han G, Gupta SD, Zhang Z, Yue J, Dong F, Gable K, Niranjanakumari S, Li W, Wang L, Liu W, Yao R, Cahoon EB, Dunn TM, Gong X
Sci Adv (2023) 9 pp. eadg0728-eadg0728 [ DOI: doi:10.1126/sciadv.adg0728 Pubmed: 36989369 ]
- Ormdl family protein (18 kDa, Protein from Arabidopsis thaliana)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Long chain base biosynthesis protein 2a (54 kDa, Protein from Arabidopsis thaliana)
- N-[(2s,3r,4e)-1,3-dihydroxyoctadec-4-en-2-yl]tetracosanamide (650 Da, Ligand)
- Spt-orm1 complex (Complex from Arabidopsis thaliana)
- Long chain base biosynthesis protein 1 (53 kDa, Protein from Arabidopsis thaliana)
- Transmembrane protein, putative (duf3317) (9 kDa, Protein from Arabidopsis thaliana)
Cryo-EM structure of the monomeric atSPT-ORM1 complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7yjm
Q-score: 0.585
Liu P, Xie T, Wu X, Han G, Gupta SD, Zhang Z, Yue J, Dong F, Gable K, Niranjanakumari S, Li W, Wang L, Liu W, Yao R, Cahoon EB, Dunn TM, Gong X
Sci Adv (2023) 9 pp. eadg0728-eadg0728 [ DOI: doi:10.1126/sciadv.adg0728 Pubmed: 36989369 ]
- Ormdl family protein (18 kDa, Protein from Arabidopsis thaliana)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Long chain base biosynthesis protein 2a (54 kDa, Protein from Arabidopsis thaliana)
- Atlcb1 (6 kDa, Protein from Arabidopsis thaliana)
- N-[(2s,3r,4e)-1,3-dihydroxyoctadec-4-en-2-yl]tetracosanamide (650 Da, Ligand)
- Spt-orm1 complex (Complex from Arabidopsis thaliana)
- Transmembrane protein, putative (duf3317) (9 kDa, Protein from Arabidopsis thaliana)
- Atlcb1 (46 kDa, Protein from Arabidopsis thaliana)
Cryo-EM structure of the monomeric atSPT-ORM1 (ORM1-N17A) complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7yjn
Q-score: 0.526
Liu P, Xie T, Wu X, Han G, Gupta SD, Zhang Z, Yue J, Dong F, Gable K, Niranjanakumari S, Li W, Wang L, Liu W, Yao R, Cahoon EB, Dunn TM, Gong X
Sci Adv (2023) 9 pp. eadg0728-eadg0728 [ DOI: doi:10.1126/sciadv.adg0728 Pubmed: 36989369 ]
- Long chain base biosynthesis protein 1 (6 kDa, Protein from Arabidopsis thaliana)
- Long chain base biosynthesis protein 1 (46 kDa, Protein from Arabidopsis thaliana)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Long chain base biosynthesis protein 2a (54 kDa, Protein from Arabidopsis thaliana)
- Ormdl family protein (18 kDa, Protein from Arabidopsis thaliana)
- Spt-orm1 complex (Complex from Arabidopsis thaliana)
- Transmembrane protein, putative (duf3317) (9 kDa, Protein from Arabidopsis thaliana)
Cryo-EM structure of the monomeric atSPT-ORM1 (LCB2a-deltaN5) complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7yjo
Q-score: 0.621
Liu P, Xie T, Wu X, Han G, Gupta SD, Zhang Z, Yue J, Dong F, Gable K, Niranjanakumari S, Li W, Wang L, Liu W, Yao R, Cahoon EB, Dunn TM, Gong X
Sci Adv (2023) 9 pp. eadg0728-eadg0728 [ DOI: doi:10.1126/sciadv.adg0728 Pubmed: 36989369 ]
- Ormdl family protein (18 kDa, Protein from Arabidopsis thaliana)
- Long chain base biosynthesis protein 2a (53 kDa, Protein from Arabidopsis thaliana)
- Atlcb1 (6 kDa, Protein from Arabidopsis thaliana)
- N-[(2s,3r,4e)-1,3-dihydroxyoctadec-4-en-2-yl]tetracosanamide (650 Da, Ligand)
- Spt-orm1 complex (Complex from Arabidopsis thaliana)
- Transmembrane protein, putative (duf3317) (9 kDa, Protein from Arabidopsis thaliana)
- Atlcb1 (46 kDa, Protein from Arabidopsis thaliana)
- [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine (336 Da, Ligand)