Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gajić J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R
Nature (2024) 632 pp. 664-671 [ DOI: 10.1038/s41586-024-07720-6 Pubmed: 39048819 ]
Image sets:
Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gajic J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R
Nature (2024) 632 pp. 664-671 [ DOI: doi:10.1038/s41586-024-07720-6 Pubmed: 39048819 ]
Native eisosome lattice bound to plasma membrane microdomain
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8qb7
Q-score: 0.323
Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gajic J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R
Nature (2024) 632 pp. 664-671 [ DOI: doi:10.1038/s41586-024-07720-6 Pubmed: 39048819 ]
Resolution: 3.35 Å
EM Method: Helical reconstruction
Fitted PDBs: 8qb9
Q-score: 0.478
Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gajic J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R
Nature (2024) 632 pp. 664-671 [ DOI: doi:10.1038/s41586-024-07720-6 Pubmed: 39048819 ]
Resolution: 3.86 Å
EM Method: Helical reconstruction
Fitted PDBs: 8qbb
Q-score: 0.404
Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gajic J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R
Nature (2024) 632 pp. 664-671 [ DOI: doi:10.1038/s41586-024-07720-6 Pubmed: 39048819 ]
- D-myo-inositol-1,4,5-triphosphate (420 Da, Ligand)
- Sphingolipid long chain base-responsive protein pil1 (38 kDa, Protein from Saccharomyces cerevisiae)
- Helical assembly of recombinant pil1 protein tubulating +pip2/-sterol lipid mixture (dopc,dope,dops,pi(4,5)p2 50:20:20:10) (Complex from Saccharomyces cerevisiae)
Resolution: 3.61 Å
EM Method: Helical reconstruction
Fitted PDBs: 8qbd
Q-score: 0.428
Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gajic J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R
Nature (2024) 632 pp. 664-671 [ DOI: doi:10.1038/s41586-024-07720-6 Pubmed: 39048819 ]
- O-[(r)-{[(2r)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-l-serine (792 Da, Ligand)
- Helical assembly of recombinant pil1 protein tubulating +pip2/+sterol lipid mixture (dopc, dope, dops, cholesterol, pi(4,5)p2 35:20:20:15:10) (Complex from Saccharomyces cerevisiae)
- D-myo-inositol-1,4,5-triphosphate (420 Da, Ligand)
- Sphingolipid long chain base-responsive protein pil1 (38 kDa, Protein from Saccharomyces cerevisiae)
Compact state - Native eisosome lattice bound to plasma membrane microdomain
Resolution: 3.67 Å
EM Method: Single-particle
Fitted PDBs: 8qbe
Q-score: 0.228
Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gajic J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R
Nature (2024) 632 pp. 664-671 [ DOI: doi:10.1038/s41586-024-07720-6 Pubmed: 39048819 ]
Stretched state - Native eisosome lattice bound to plasma membrane microdomain
Resolution: 3.64 Å
EM Method: Single-particle
Fitted PDBs: 8qbg
Q-score: 0.217
Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gajic J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R
Nature (2024) 632 pp. 664-671 [ DOI: doi:10.1038/s41586-024-07720-6 Pubmed: 39048819 ]
Cryo-EM structure of the SEA complex (consensus map)
Resolution: 2.95 Å
EM Method: Single-particle
Fitted PDBs: 8adl
Q-score: 0.218
Tafur L, Hinterndorfer K, Gabus C, Lamanna C, Bergmann A, Sadian Y, Hamdi F, Kyrilis FL, Kastritis PL, Loewith R
Nature (2022) 611 pp. 399-404 [ DOI: doi:10.1038/s41586-022-05370-0 Pubmed: 36289347 ]
- Maintenance of telomere capping protein 5 (131 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin seh1 (39 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Nitrogen permease regulator 3 (130 kDa, Protein from Saccharomyces cerevisiae)
- Seh1-associated complex (seac) (2 MDa, Complex from Saccharomyces cerevisiae)
- Nitrogen permease regulator 2 (69 kDa, Protein from Saccharomyces cerevisiae)
- Vacuolar membrane-associated protein iml1 (182 kDa, Protein from Saccharomyces cerevisiae)
- Seh-associated protein 4 (117 kDa, Protein from Saccharomyces cerevisiae)
- Protein transport protein sec13 (33 kDa, Protein from Saccharomyces cerevisiae)
- Restriction of telomere capping protein 1 (149 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of the SEA complex (protomer focused map)
Tafur L, Hinterndorfer K, Gabus C, Lamanna C, Bergmann A, Sadian Y, Hamdi F, Kyrilis FL, Kastritis PL, Loewith R
Nature (2022) 611 pp. 399-404 [ DOI: doi:10.1038/s41586-022-05370-0 Pubmed: 36289347 ]