Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gajic J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R
Nature (2024) 632 pp. 664-671 [ DOI: doi:10.1038/s41586-024-07720-6 Pubmed: 39048819 ]
Native eisosome lattice bound to plasma membrane microdomain
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8qb7
Q-score: 0.323
Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gajic J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R
Nature (2024) 632 pp. 664-671 [ DOI: doi:10.1038/s41586-024-07720-6 Pubmed: 39048819 ]
Resolution: 3.35 Å
EM Method: Helical reconstruction
Fitted PDBs: 8qb9
Q-score: 0.478
Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gajic J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R
Nature (2024) 632 pp. 664-671 [ DOI: doi:10.1038/s41586-024-07720-6 Pubmed: 39048819 ]
Resolution: 3.86 Å
EM Method: Helical reconstruction
Fitted PDBs: 8qbb
Q-score: 0.404
Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gajic J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R
Nature (2024) 632 pp. 664-671 [ DOI: doi:10.1038/s41586-024-07720-6 Pubmed: 39048819 ]
- D-myo-inositol-1,4,5-triphosphate (420 Da, Ligand)
- Sphingolipid long chain base-responsive protein pil1 (38 kDa, Protein from Saccharomyces cerevisiae)
- Helical assembly of recombinant pil1 protein tubulating +pip2/-sterol lipid mixture (dopc,dope,dops,pi(4,5)p2 50:20:20:10) (Complex from Saccharomyces cerevisiae)
Resolution: 3.61 Å
EM Method: Helical reconstruction
Fitted PDBs: 8qbd
Q-score: 0.428
Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gajic J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R
Nature (2024) 632 pp. 664-671 [ DOI: doi:10.1038/s41586-024-07720-6 Pubmed: 39048819 ]
- O-[(r)-{[(2r)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-l-serine (792 Da, Ligand)
- Helical assembly of recombinant pil1 protein tubulating +pip2/+sterol lipid mixture (dopc, dope, dops, cholesterol, pi(4,5)p2 35:20:20:15:10) (Complex from Saccharomyces cerevisiae)
- D-myo-inositol-1,4,5-triphosphate (420 Da, Ligand)
- Sphingolipid long chain base-responsive protein pil1 (38 kDa, Protein from Saccharomyces cerevisiae)
Compact state - Native eisosome lattice bound to plasma membrane microdomain
Resolution: 3.67 Å
EM Method: Single-particle
Fitted PDBs: 8qbe
Q-score: 0.228
Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gajic J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R
Nature (2024) 632 pp. 664-671 [ DOI: doi:10.1038/s41586-024-07720-6 Pubmed: 39048819 ]
Stretched state - Native eisosome lattice bound to plasma membrane microdomain
Resolution: 3.64 Å
EM Method: Single-particle
Fitted PDBs: 8qbg
Q-score: 0.217
Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gajic J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R
Nature (2024) 632 pp. 664-671 [ DOI: doi:10.1038/s41586-024-07720-6 Pubmed: 39048819 ]
Cryo-EM structure of Saccharomyces cerevisiae TOROID (TORC1 Organized in Inhibited Domains).
Resolution: 3.87 Å
EM Method: Single-particle
Fitted PDBs: 7pqh
Q-score: 0.241
Prouteau M, Bourgoint C, Felix J, Bonadei L, Sadian Y, Gabus C, Savvides SN, Gutsche I, Desfosses A, Loewith R
Nat Struct Mol Biol (2023) 30 pp. 273-285 [ DOI: doi:10.1038/s41594-022-00912-6 Pubmed: 36702972 ]
- Serine/threonine-protein kinase tor2 (281 kDa, Protein from Saccharomyces cerevisiae)
- Target of rapamycin complex 1 subunit kog1,target of rapamycin complex 1 subunit kog1 (183 kDa, Protein from Saccharomyces cerevisiae)
- Target of rapamycin complex subunit lst8 (34 kDa, Protein from Saccharomyces cerevisiae)
- Torc1 organized in inhibited domains (toroid) (Complex from Saccharomyces cerevisiae)
Helical reconstruction of TOROID (TORC1 Organized in Inhibited Domains) filaments.
Prouteau M, Bourgoint C, Felix J, Bonadei L, Sadian Y, Gabus C, Savvides SN, Gutsche I, Desfosses A, Loewith R
Nat Struct Mol Biol (2023) 30 pp. 273-285 [ DOI: doi:10.1038/s41594-022-00912-6 Pubmed: 36702972 ]
- Target of rapamycin complex 1 subunit kog1 (Protein from Saccharomyces cerevisiae)
- Target of rapamycin complex subunit lst8 (Protein from Saccharomyces cerevisiae)
- Serine/threonine-protein kinase tor2 (Protein from Saccharomyces cerevisiae)
- Torc1 organized in inhibited domains (toroid) (Complex from Saccharomyces cerevisiae)
Cryo-EM structure of the SEA complex (consensus map)
Resolution: 2.95 Å
EM Method: Single-particle
Fitted PDBs: 8adl
Q-score: 0.218
Tafur L, Hinterndorfer K, Gabus C, Lamanna C, Bergmann A, Sadian Y, Hamdi F, Kyrilis FL, Kastritis PL, Loewith R
Nature (2022) 611 pp. 399-404 [ DOI: doi:10.1038/s41586-022-05370-0 Pubmed: 36289347 ]
- Maintenance of telomere capping protein 5 (131 kDa, Protein from Saccharomyces cerevisiae)
- Nucleoporin seh1 (39 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Nitrogen permease regulator 3 (130 kDa, Protein from Saccharomyces cerevisiae)
- Seh1-associated complex (seac) (2 MDa, Complex from Saccharomyces cerevisiae)
- Nitrogen permease regulator 2 (69 kDa, Protein from Saccharomyces cerevisiae)
- Vacuolar membrane-associated protein iml1 (182 kDa, Protein from Saccharomyces cerevisiae)
- Seh-associated protein 4 (117 kDa, Protein from Saccharomyces cerevisiae)
- Protein transport protein sec13 (33 kDa, Protein from Saccharomyces cerevisiae)
- Restriction of telomere capping protein 1 (149 kDa, Protein from Saccharomyces cerevisiae)