Retron-Eco1 filament with ADP-ribosylated Effector (local map with 1 segment)
Resolution: 2.99 Å
EM Method: Single-particle
Fitted PDBs: 8qbk
Q-score: 0.468
Carabias A, Camara-Wilpert S, Mestre MR, Lopez-Mendez B, Hendriks IA, Zhao R, Pape T, Fuglsang A, Luk SH, Nielsen ML, Pinilla-Redondo R, Montoya G
Mol Cell (2024) 84 pp. 2185- [ DOI: doi:10.1016/j.molcel.2024.05.001 Pubmed: 38788717 ]
- [(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-dihydroxy-oxolan-2-yl]methyl [hydroxy-[[(2r,3s,4r,5s)-3,4,5-trihydroxyoxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate (559 Da, Ligand)
- Filamentous assembly of retron-eco1 in complex with adpr, containing rt-msr-msdna-effector complex (1 segment) (21 MDa, Complex from Escherichia coli BL21(DE3))
- Retron ec86 reverse transcriptase (39 kDa, Protein from Escherichia coli BL21(DE3))
- Magnesium ion (24 Da, Ligand)
- Retron-eco1-msr (26 kDa, RNA from Escherichia coli BL21(DE3))
- Retron-eco1-a2 (4 kDa, RNA from Escherichia coli BL21(DE3))
- Retron-eco1 msdna (26 kDa, DNA from Escherichia coli BL21(DE3))
- Retron ec86 putative ribosyltransferase/dna-binding protein (35 kDa, Protein from Escherichia coli BL21(DE3))
Retron-Eco1 filament with inactive effector (E106A, 2 segments)
Resolution: 2.66 Å
EM Method: Single-particle
Fitted PDBs: 8qbl
Q-score: 0.387
Carabias A, Camara-Wilpert S, Mestre MR, Lopez-Mendez B, Hendriks IA, Zhao R, Pape T, Fuglsang A, Luk SH, Nielsen ML, Pinilla-Redondo R, Montoya G
Mol Cell (2024) 84 pp. 2185- [ DOI: doi:10.1016/j.molcel.2024.05.001 Pubmed: 38788717 ]
- Retron ec86 reverse transcriptase (39 kDa, Protein from Escherichia coli BL21(DE3))
- Magnesium ion (24 Da, Ligand)
- Retron-eco1-msr (26 kDa, RNA from Escherichia coli BL21(DE3))
- Retron-eco1-a2 (4 kDa, RNA from Escherichia coli BL21(DE3))
- Retron-eco1 msdna (26 kDa, DNA from Escherichia coli BL21(DE3))
- Filamentous assembly of retron-eco1-e106a mutant containing rt-msr-msdna-effector complex (2 segments) (21 MDa, Complex from Escherichia coli BL21(DE3))
- Retron ec86 putative ribosyltransferase/dna-binding protein (35 kDa, Protein from Escherichia coli BL21(DE3))
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
Retron-Eco1 filament with ADP-ribosylated Effector (full map with 2 segments)
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 8qbm
Q-score: 0.365
Carabias A, Camara-Wilpert S, Mestre MR, Lopez-Mendez B, Hendriks IA, Zhao R, Pape T, Fuglsang A, Luk SH, Nielsen ML, Pinilla-Redondo R, Montoya G
Mol Cell (2024) 84 pp. 2185- [ DOI: doi:10.1016/j.molcel.2024.05.001 Pubmed: 38788717 ]
- [(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-dihydroxy-oxolan-2-yl]methyl [hydroxy-[[(2r,3s,4r,5s)-3,4,5-trihydroxyoxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate (559 Da, Ligand)
- Retron ec86 reverse transcriptase (39 kDa, Protein from Escherichia coli BL21(DE3))
- Retron-eco1-msr (26 kDa, RNA from Escherichia coli BL21(DE3))
- Retron-eco1-a2 (4 kDa, RNA from Escherichia coli BL21(DE3))
- Retron-eco1 msdna (26 kDa, DNA from Escherichia coli BL21(DE3))
- Filamentous assembly of retron-eco1 in complex with adpr, containing rt-msr-msdna-effector complex (2 segments) (21 MDa, Complex from Escherichia coli BL21(DE3))
- Magnesium ion (24 Da, Ligand from Escherichia coli BL21(DE3))
- Retron ec86 putative ribosyltransferase/dna-binding protein (35 kDa, Protein from Escherichia coli BL21(DE3))
Retron-Eco1 filament (2 segments)
Carabias A, Camara-Wilpert S, Mestre MR, Lopez-Mendez B, Hendriks IA, Zhao R, Pape T, Fuglsang A, Luk SH, Nielsen ML, Pinilla-Redondo R, Montoya G
Mol Cell (2024) 84 pp. 2185-2202.e12 [ DOI: doi:10.1016/j.molcel.2024.05.001 Pubmed: 38788717 ]
- Retron-eco1 reverse transcriptase (Protein from Escherichia coli BL21(DE3))
- Filamentous assembly containing retron-eco1 rt:ncrna-msdna:effector with a stoichiometry of 2:2:2 per repetitive unit - 2 segments (21 MDa, Complex from Escherichia coli BL21(DE3))
- Retron-eco1 effector (Protein from Escherichia coli BL21(DE3))
- Retron-eco1-msr (RNA from Escherichia coli BL21(DE3))
- Retron-eco1 msdna (Macromolecule from Escherichia coli BL21(DE3))
- Retron-eco1-a2 (RNA from Escherichia coli BL21(DE3))
Retron-Eco1 -1 turn mutant filament with ADP-ribosylated Effector (Consensus refinement)
Carabias A, Camara-Wilpert S, Mestre MR, Lopez-Mendez B, Hendriks IA, Zhao R, Pape T, Fuglsang A, Luk SH, Nielsen ML, Pinilla-Redondo R, Montoya G
Mol Cell (2024) 84 pp. 2185-2202.e12 [ DOI: doi:10.1016/j.molcel.2024.05.001 Pubmed: 38788717 ]
- Retron-eco1 reverse transcriptase (Protein from Escherichia coli BL21(DE3))
- Retron-eco1-msr (RNA from Escherichia coli BL21(DE3))
- Filamentous assembly of retron-eco1 "-1 turn" mutant in complex with adpr, containing rt-msr-msdna-effector complex (consensus refinement) (21 MDa, Complex from Escherichia coli BL21(DE3))
- Retron-eco1-msdna "-1 turn" (DNA from Escherichia coli BL21(DE3))
- Retron-eco1-a2 (RNA from Escherichia coli BL21(DE3))
- Retron-eco1-effector (Protein from Escherichia coli BL21(DE3))
Retron-Eco1 filament with ADP-ribosylated Effector (Consensus refinement)
Carabias A, Camara-Wilpert S, Mestre MR, Lopez-Mendez B, Hendriks IA, Zhao R, Pape T, Fuglsang A, Luk SH, Nielsen ML, Pinilla-Redondo R, Montoya G
Mol Cell (2024) 84 pp. 2185-2202.e12 [ DOI: doi:10.1016/j.molcel.2024.05.001 Pubmed: 38788717 ]
- Retron-eco1 reverse transcriptase (Protein from Escherichia coli BL21(DE3))
- Filamentous assembly of retron-eco1 in complex with adpr, containing rt-msr-msdna-effector complex (consensus refinement) (21 MDa, Complex from Escherichia coli BL21(DE3))
- Retron-eco1 msdna (DNA from Escherichia coli BL21(DE3))
- Retron-eco1 effector (Protein from Escherichia coli BL21(DE3))
- Retron-eco1-msr (RNA from Escherichia coli BL21(DE3))
- Retron-eco1-a2 (RNA from Escherichia coli BL21(DE3))
Resolution: 2.46 Å
EM Method: Single-particle
Fitted PDBs: 8aa5
Q-score: 0.519
Tenjo-Castano F, Sofos N, Lopez-Mendez B, Stutzke LS, Fuglsang A, Stella S, Montoya G
Nat Commun (2022) 13 pp. 5792-5792 [ DOI: doi:10.1038/s41467-022-33504-5 Pubmed: 36184667 ]
- Water (18 Da, Ligand)
- Re_target (24 kDa, DNA from Scytonema hofmannii)
- Le_polya (23 kDa, DNA from Scytonema hofmannii)
- Target_1 (4 kDa, DNA from Scytonema hofmannii)
- Le_target (24 kDa, DNA from Scytonema hofmannii)
- Re_polya (22 kDa, DNA from Scytonema hofmannii)
- Tnsb (68 kDa, Protein from Scytonema hofmannii)
- Target_2 (4 kDa, DNA from Scytonema hofmannii)
- Ternary complex of shtnsb transposase with strand transfer complex dna. (359 kDa, Complex from Scytonema hofmannii)
Type V-K CAST TnsB bound to LTR-SR
Tenjo-Castano F, Sofos N, Lopez-Mendez B, Stutzke LS, Fuglsang A, Stella S, Montoya G
Nat Commun (2022) 13 pp. 5792-5792 [ DOI: doi:10.1038/s41467-022-33504-5 Pubmed: 36184667 ]
- Tnsb (Protein from Scytonema hofmannii)
- Rightelement (DNA from Scytonema hofmannii)
- Leftelement (DNA from Scytonema hofmannii)
- Type v-k cast tnsb bound to ltr-sr (359 kDa, Complex from Scytonema hofmannii)
Reconstruction of the CRISPR-associated hexameric RNase Csx1 from Sulfolobus islandicus
Molina R, Stella S, Feng M, Sofos N, Jauniskis V, Pozdnyakova I, Lopez-Mendez B, She Q, Montoya G
Nat Commun (2019) 10 pp. 4302-4302 [ Pubmed: 31541109 DOI: doi:10.1038/s41467-019-12244-z ]
- Csx1 hexamer (320 kDa, Complex from Sulfolobus islandicus REY15A)