Cryo-EM structure of lipoprotein-bound LolCDE in nanodiscs
Qiao W, Shen C, Chen Y, Chang S, Wang X, Yang L, Pang J, Luo Q, Zhang Z, Xiang Y, Zhao C, Lu G, Ding BS, Ying B, Tang X, Dong H
Signal Transduct Target Ther (2024) 9 pp. 354-354 [ DOI: doi:10.1038/s41392-024-02067-w Pubmed: 39725716 ]
- Complex of endogenous atp-bound lolcde in nanodiscs (Complex from Escherichia coli K-12)
- Lipoprotein(lpp) (1 kDa, Protein from Escherichia coli K-12)
- Palmitic acid (256 Da, Ligand)
- Lipoprotein-releasing system atp-binding protein lold (26 kDa, Protein from Escherichia coli K-12)
- (2s)-3-hydroxypropane-1,2-diyl dihexadecanoate (568 Da, Ligand)
- Lipoprotein-releasing system transmembrane protein lolc (43 kDa, Protein from Escherichia coli K-12)
- Lipoprotein-releasing system transmembrane protein lole (45 kDa, Protein from Escherichia coli K-12)
Cryo-EM structure of endogenous ATP-bound LolCDE with LolD-E171Q mutations in nanodiscs
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 9gvk
Q-score: 0.472
Qiao W, Shen C, Chen Y, Chang S, Wang X, Yang L, Pang J, Luo Q, Zhang Z, Xiang Y, Zhao C, Lu G, Ding BS, Ying B, Tang X, Dong H
Signal Transduct Target Ther (2024) 9 pp. 354-354 [ DOI: doi:10.1038/s41392-024-02067-w Pubmed: 39725716 ]
- Lipoprotein-releasing system transmembrane protein lolc (43 kDa, Protein from Escherichia coli K-12)
- Lipoprotein-releasing system atp-binding protein lold (26 kDa, Protein from Escherichia coli K-12)
- Lipoprotein-releasing system transmembrane protein lole (45 kDa, Protein from Escherichia coli K-12)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Complex of endogenous atp-bound lolcde with lold-e171q mutations in nanodiscs (Complex from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
Zuo H, Park J, Frangaj A, Ye J, Lu G, Manning JJ, Asher WB, Lu Z, Hu GB, Wang L, Mendez J, Eng E, Zhang Z, Lin X, Grassucci R, Hendrickson WA, Clarke OB, Javitch JA, Conigrave AD, Fan QR
Nature (2024) [ DOI: 10.1038/s41586-024-07331-1 Pubmed: 38632411 ]
Image sets:
Structure of human calcium-sensing receptor in complex with miniGi1 protein in detergent
Zuo H, Park J, Frangaj A, Ye J, Lu G, Manning JJ, Asher WB, Lu Z, Hu GB, Wang L, Mendez J, Eng E, Zhang Z, Lin X, Grassucci R, Hendrickson WA, Clarke OB, Javitch JA, Conigrave AD, Fan QR
Nature (2024) 629 pp. 481-488 [ DOI: 10.1038/s41586-024-07331-1 Pubmed: 38632411 ]
Image sets:
Structure of human calcium-sensing receptor in complex with Gi3 protein in nanodiscs
Zuo H, Park J, Frangaj A, Ye J, Lu G, Manning JJ, Asher WB, Lu Z, Hu GB, Wang L, Mendez J, Eng E, Zhang Z, Lin X, Grassucci R, Hendrickson WA, Clarke OB, Javitch JA, Conigrave AD, Fan QR
Nature (2024) [ DOI: 10.1038/s41586-024-07331-1 Pubmed: 38632411 ]
Image sets:
Zuo H, Park J, Frangaj A, Ye J, Lu G, Manning JJ, Asher WB, Lu Z, Hu GB, Wang L, Mendez J, Eng E, Zhang Z, Lin X, Grassucci R, Hendrickson WA, Clarke OB, Javitch JA, Conigrave AD, Fan QR
Nature (2024) [ DOI: 10.1038/s41586-024-07331-1 Pubmed: 38632411 ]
Image sets:
Zuo H, Park J, Frangaj A, Ye J, Lu G, Manning JJ, Asher WB, Lu Z, Hu G, Wang L, Mendez J, Eng E, Zhang Z, Lin X, Grasucci R, Hendrickson WA, Clarke OB, Javitch JA, Conigrave AD, Fan QR
To Be Published
Image sets:
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 9asb
Q-score: 0.524
Zuo H, Park J, Frangaj A, Ye J, Lu G, Manning JJ, Asher WB, Lu Z, Hu GB, Wang L, Mendez J, Eng E, Zhang Z, Lin X, Grassucci R, Hendrickson WA, Clarke OB, Javitch JA, Conigrave AD, Fan QR
Nature (2024) 629 pp. 481-488 [ DOI: doi:10.1038/s41586-024-07331-1 Pubmed: 38632411 ]
- Chimeric mini guanine nucleotide-binding protein g(i)(s)(q) subunit alpha (28 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- (19r,22s,25r)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9z)-octadec-9-enoate (749 Da, Ligand)
- Human casr in complex with chimeric gq (minigisq) protein (276 kDa, Complex from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Phosphate ion (94 Da, Ligand)
- Isoform 1 of extracellular calcium-sensing receptor (102 kDa, Protein from Homo sapiens)
- 3-(2-chlorophenyl)-n-[(1r)-1-(3-methoxyphenyl)ethyl]propan-1-amine (303 Da, Ligand)
- Cyclomethyltryptophan (216 Da, Ligand)
- Chimeric mini guanine nucleotide-binding protein g(i)(s)(q) subunit alpha (7 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
Consensus map of human CaSR-miniGisq complex in nanodiscs
Zuo H, Park J, Frangaj A, Ye J, Lu G, Manning JJ, Asher WB, Lu Z, Hu GB, Wang L, Mendez J, Eng E, Zhang Z, Lin X, Grassucci R, Hendrickson WA, Clarke OB, Javitch JA, Conigrave AD, Fan QR
Nature (2024) 629 pp. 481-488 [ Pubmed: 38632411 DOI: doi:10.1038/s41586-024-07331-1 ]
Zuo H, Park J, Frangaj A, Ye J, Lu G, Manning JJ, Asher WB, Lu Z, Hu GB, Wang L, Mendez J, Eng E, Zhang Z, Lin X, Grassucci R, Hendrickson WA, Clarke OB, Javitch JA, Conigrave AD, Fan QR
Nature (2024) 629 pp. 481-488 [ Pubmed: 38632411 DOI: doi:10.1038/s41586-024-07331-1 ]