Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7r0w
Q-score: 0.523
Proctor MS, Malone LA, Farmer DA, Swainsbury DJK, Hawkings FR, Pastorelli F, Emrich-Mills TZ, Siebert CA, Hunter CN, Johnson MP, Hitchcock A
Biochem J (2022) 479 pp. 1487-1503 [ Pubmed: 35726684 DOI: doi:10.1042/BCJ20220124 ]
- (4s,7r)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Cytochrome b6f complex in association with petp (Complex from Synechocystis sp. PCC 6803)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Beta,beta-caroten-4-one (550 Da, Ligand)
- Uncharacterized protein cp097, conserved in cyanobacteria (7 kDa, Protein from Synechocystis)
- 2,3-dimethyl-5-(3,7,11,15,19,23,27,31,35-nonamethyl-2,6,10,14,18,22,26,30,34-hexatriacontanonaenyl-2,5-cyclohexadiene-1,4-dione-2,3-dimethyl-5-solanesyl-1,4-benzoquinone (749 Da, Ligand)
- Heme c (618 Da, Ligand)
- Cytochrome b6-f complex subunit 5 (4 kDa, Protein from Synechocystis sp. PCC 6803)
- Cytochrome b6-f complex subunit 4 (17 kDa, Protein from Synechocystis sp. PCC 6803)
- (1r)-2-{[{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl (11e)-octadec-11-enoate (749 Da, Ligand)
- Water (18 Da, Ligand)
- Cytochrome b6-f complex subunit 7 (3 kDa, Protein from Synechocystis sp. PCC 6803)
- (1s,8e)-1-{[(2s)-1-hydroxy-3-{[(1s)-1-hydroxypentadecyl]oxy}propan-2-yl]oxy}heptadec-8-en-1-ol (570 Da, Ligand)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Rieske domain, petc (20 kDa, Protein from Synechocystis sp. PCC 6803)
- Cytochrome b6 (25 kDa, Protein from Synechocystis sp. PCC 6803)
- Cytochrome f (35 kDa, Protein from Synechocystis sp. PCC 6803)
- Cytochrome b6 (3 kDa, Protein from Synechocystis sp. PCC 6803)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Cytochrome b6-f complex subunit 8 (3 kDa, Protein from Synechocystis sp. PCC 6803)
- Chlorophyll a (893 Da, Ligand)
- Eicosane (282 Da, Ligand)
Resolution: 3.15 Å
EM Method: Single-particle
Fitted PDBs: 7zxy
Q-score: 0.54
Proctor MS, Malone LA, Farmer DA, Swainsbury DJK, Hawkings FR, Pastorelli F, Emrich-Mills TZ, Siebert CA, Hunter CN, Johnson MP, Hitchcock A
Biochem J (2022) 479 pp. 1487-1503 [ Pubmed: 35726684 DOI: doi:10.1042/BCJ20220124 ]
- Cytochrome b6 (25 kDa, Protein from Synechocystis sp. PCC 6803)
- Cytochrome b6 (3 kDa, Protein from Synechocystis sp. PCC 6803)
- Cytochrome b6-f complex iron-sulfur subunit 2 (19 kDa, Protein from Synechocystis sp. PCC 6803)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Cytochrome b6-f complex subunit 7 (3 kDa, Protein from Synechocystis sp. PCC 6803)
- Cytochrome b6-f complex subunit 5 (4 kDa, Protein from Synechocystis sp. PCC 6803)
- Beta,beta-caroten-4-one (550 Da, Ligand)
- Cytochrome b6f with natively associated lipids and plastoquinone (Complex from Synechocystis sp. PCC 6803)
- Cytochrome b6-f complex subunit 8 (3 kDa, Protein from Synechocystis sp. PCC 6803)
- Cytochrome f (30 kDa, Protein from Synechocystis sp. PCC 6803)
- Chlorophyll a (893 Da, Ligand)
- Eicosane (282 Da, Ligand)
- Cytochrome b6-f complex subunit 4 (17 kDa, Protein from Synechocystis sp. PCC 6803)
- Heme c (618 Da, Ligand)
- (1r)-2-{[{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl (11e)-octadec-11-enoate (749 Da, Ligand)
RC-LH1(16) complex from Rhodopseudomonas palustris
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 6z5r
Q-score: 0.603
Swainsbury DJK, Qian P, Jackson PJ, Faries KM, Niedzwiedzki DM, Martin EC, Farmer DA, Malone LA, Thompson RF, Ranson NA, Canniffe DP, Dickman MJ, Holten D, Kirmaier C, Hitchcock A, Hunter CN
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abe2631 Pubmed: 33523887 ]
- H subunit of photosynthetic reaction center complex (27 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- (6~{r},10~{s},14~{r},19~{r},23~{s},24~{e},27~{s},28~{e})-2,6,10,14,19,23,27,31-octamethyldotriaconta-24,28-dien-2-ol (575 Da, Ligand)
- Bacteriochlorophyll a (911 Da, Ligand)
- (4s,7r)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Reaction center-light harvesting complex 1 (3 MDa, Complex from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Reaction center protein m chain (34 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Bacteriopheophytin a (889 Da, Ligand)
- Reaction center protein l chain (30 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Spirilloxanthin (596 Da, Ligand)
- Ubiquinone-10 (863 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Light-harvesting complex 1 alpha chain (5 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Light-harvesting complex 1 beta chain (5 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Water (18 Da, Ligand)
- Fe (iii) ion (55 Da, Ligand)
RC-LH1(14)-W complex from Rhodopseudomonas palustris
Resolution: 2.65 Å
EM Method: Single-particle
Fitted PDBs: 6z5s
Q-score: 0.593
Swainsbury DJK, Qian P, Jackson PJ, Faries KM, Niedzwiedzki DM, Martin EC, Farmer DA, Malone LA, Thompson RF, Ranson NA, Canniffe DP, Dickman MJ, Holten D, Kirmaier C, Hitchcock A, Hunter CN
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abe2631 Pubmed: 33523887 ]
- H subunit of photosynthetic reaction center complex (27 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- (6~{r},10~{s},14~{r},19~{r},23~{s},24~{e},27~{s},28~{e})-2,6,10,14,19,23,27,31-octamethyldotriaconta-24,28-dien-2-ol (575 Da, Ligand)
- Bacteriochlorophyll a (911 Da, Ligand)
- (4s,7r)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Reaction center-light harvesting complex 1-protein w (3 MDa, Complex from Rhodopseudomonas palustris CGA009)
- Light-harvesting complex 1 beta chain (7 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Light harvesting complex 1 protein w (10 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Reaction center protein m chain (34 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Light-harvesting complex 1 alpha chain (7 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Ubiquinone-10 (863 Da, Ligand)
- Spirilloxanthin (596 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- Bacteriopheophytin a (889 Da, Ligand)
- Reaction center protein l chain (30 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Water (18 Da, Ligand)
- Fe (iii) ion (55 Da, Ligand)
Resolution: 3.58 Å
EM Method: Single-particle
Fitted PDBs: 6rqf
Q-score: 0.511
Malone LA, Qian P, Mayneord GE, Hitchcock A, Farmer DA, Thompson RF, Swainsbury DJK, Ranson NA, Hunter CN, Johnson MP
Nature (2019) 575 pp. 535-539 [ DOI: doi:10.1038/s41586-019-1746-6 Pubmed: 31723268 ]
- (4s,7r)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Cytochrome f (31 kDa, Protein from Spinach)
- Cytochrome b6-f complex subunit 6 (3 kDa, Protein from Spinach)
- Cytochrome b6 (24 kDa, Protein from Spinach)
- Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (18 kDa, Protein from Spinach)
- Cytochrome b6-f complex subunit 7 (3 kDa, Protein from Spinach)
- Cytochrome b6-f complex subunit 8 (3 kDa, Protein from Spinach)
- Spinach cytochrome b6f complex with native bound plastoquinone and thylakoid lipids (Complex from Spinacia oleracea)
- 2,3-dimethyl-5-(3,7,11,15,19,23,27,31,35-nonamethyl-2,6,10,14,18,22,26,30,34-hexatriacontanonaenyl-2,5-cyclohexadiene-1,4-dione-2,3-dimethyl-5-solanesyl-1,4-benzoquinone (749 Da, Ligand)
- Heme c (618 Da, Ligand)
- Beta-carotene (536 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Cytochrome b6-f complex subunit 5 (4 kDa, Protein from Spinach)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Cytochrome b6-f complex subunit 4 (17 kDa, Protein from Spinach)
- (1r)-2-{[{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl (11e)-octadec-11-enoate (749 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)