Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8asi
Q-score: 0.564
Swainsbury DJK, Hawkings FR, Martin EC, Musial S, Salisbury JH, Jackson PJ, Farmer DA, Johnson MP, Siebert CA, Hitchcock A, Hunter CN
PNAS (2023) 120 pp. e2217922120-e2217922120 [ DOI: doi:10.1073/pnas.2217922120 Pubmed: 36913593 ]
- Cytochrome b-c1 subunit iv (14 kDa, Protein from Cereibacter sphaeroides 2.4.1)
- Ubiquinol-cytochrome c reductase iron-sulfur subunit (19 kDa, Protein from Cereibacter sphaeroides 2.4.1)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Cytochrome c1 (30 kDa, Protein from Cereibacter sphaeroides 2.4.1)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Four subunit cytochrome b-c1 complex from rhodobacter sphaeroides (Complex from Cereibacter sphaeroides)
- Ubiquinone-10 (863 Da, Ligand)
- Cytochrome b (50 kDa, Protein from Cereibacter sphaeroides 2.4.1)
- Heme c (618 Da, Ligand)
Resolution: 3.75 Å
EM Method: Single-particle
Fitted PDBs: 8asj
Q-score: 0.504
Swainsbury DJK, Hawkings FR, Martin EC, Musial S, Salisbury JH, Jackson PJ, Farmer DA, Johnson MP, Siebert CA, Hitchcock A, Hunter CN
PNAS (2023) 120 pp. e2217922120-e2217922120 [ DOI: doi:10.1073/pnas.2217922120 Pubmed: 36913593 ]
- Cytochrome b-c1 subunit iv (14 kDa, Protein from Cereibacter sphaeroides 2.4.1)
- Ubiquinol-cytochrome c reductase iron-sulfur subunit (19 kDa, Protein from Cereibacter sphaeroides 2.4.1)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Cytochrome c1 (30 kDa, Protein from Cereibacter sphaeroides 2.4.1)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Four subunit cytochrome b-c1 complex from rhodobacter sphaeroides (Complex from Cereibacter sphaeroides)
- Ubiquinone-10 (863 Da, Ligand)
- Cytochrome b (50 kDa, Protein from Cereibacter sphaeroides 2.4.1)
- Heme c (618 Da, Ligand)
Cryo-EM structure of the Rhodobacter sphaeroides RC-LH1-PufXY monomer complex at 2.5 A
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7pil
Q-score: 0.64
Qian P, Swainsbury DJK, Croll TI, Salisbury JH, Martin EC, Jackson PJ, Hitchcock A, Castro-Hartmann P, Sader K, Hunter CN
Biochem J (2021) 478 pp. 3775-3790 [ Pubmed: 34590677 DOI: doi:10.1042/BCJ20210631 ]
- Light-harvesting protein b-875 beta chain (4 kDa, Protein from Rhodobacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.))
- Bacteriochlorophyll a (911 Da, Ligand)
- Reaction center protein m chain (34 kDa, Protein from Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158))
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Ubiquinone-1 (250 Da, Ligand)
- Light-harvesting protein b-875 alpha chain (6 kDa, Protein from Rhodobacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.))
- Rc-y (5 kDa, Protein from Rhodobacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.))
- (2r,5r,11r,14r)-5,8,11-trihydroxy-5,11-dioxido-17-oxo-2,14-bis(tetradecanoyloxy)-4,6,10,12,16-pentaoxa-5,11-diphosphatriacont-1-yl tetradecanoate (1 kDa, Ligand)
- Spheroidene (568 Da, Ligand)
- Reaction center protein l chain (31 kDa, Protein from Rhodobacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.))
- Intrinsic membrane protein pufx (5 kDa, Protein from Rhodobacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.))
- Reaction center protein h chain (26 kDa, Protein from Rhodobacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.))
- Bacteriopheophytin a (889 Da, Ligand)
- Ubiquinone-10 (863 Da, Ligand)
- Light harvesting complex (Complex from Cereibacter sphaeroides 2.4.1)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Water (18 Da, Ligand)
- Fe (iii) ion (55 Da, Ligand)
RC-LH1(16) complex from Rhodopseudomonas palustris
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 6z5r
Q-score: 0.603
Swainsbury DJK, Qian P, Jackson PJ, Faries KM, Niedzwiedzki DM, Martin EC, Farmer DA, Malone LA, Thompson RF, Ranson NA, Canniffe DP, Dickman MJ, Holten D, Kirmaier C, Hitchcock A, Hunter CN
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abe2631 Pubmed: 33523887 ]
- H subunit of photosynthetic reaction center complex (27 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- (6~{r},10~{s},14~{r},19~{r},23~{s},24~{e},27~{s},28~{e})-2,6,10,14,19,23,27,31-octamethyldotriaconta-24,28-dien-2-ol (575 Da, Ligand)
- Bacteriochlorophyll a (911 Da, Ligand)
- (4s,7r)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Reaction center-light harvesting complex 1 (3 MDa, Complex from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Reaction center protein m chain (34 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Bacteriopheophytin a (889 Da, Ligand)
- Reaction center protein l chain (30 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Spirilloxanthin (596 Da, Ligand)
- Ubiquinone-10 (863 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Light-harvesting complex 1 alpha chain (5 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Light-harvesting complex 1 beta chain (5 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Water (18 Da, Ligand)
- Fe (iii) ion (55 Da, Ligand)
RC-LH1(14)-W complex from Rhodopseudomonas palustris
Resolution: 2.65 Å
EM Method: Single-particle
Fitted PDBs: 6z5s
Q-score: 0.593
Swainsbury DJK, Qian P, Jackson PJ, Faries KM, Niedzwiedzki DM, Martin EC, Farmer DA, Malone LA, Thompson RF, Ranson NA, Canniffe DP, Dickman MJ, Holten D, Kirmaier C, Hitchcock A, Hunter CN
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abe2631 Pubmed: 33523887 ]
- H subunit of photosynthetic reaction center complex (27 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- (6~{r},10~{s},14~{r},19~{r},23~{s},24~{e},27~{s},28~{e})-2,6,10,14,19,23,27,31-octamethyldotriaconta-24,28-dien-2-ol (575 Da, Ligand)
- Bacteriochlorophyll a (911 Da, Ligand)
- (4s,7r)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Reaction center-light harvesting complex 1-protein w (3 MDa, Complex from Rhodopseudomonas palustris CGA009)
- Light-harvesting complex 1 beta chain (7 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Light harvesting complex 1 protein w (10 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Reaction center protein m chain (34 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Light-harvesting complex 1 alpha chain (7 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Ubiquinone-10 (863 Da, Ligand)
- Spirilloxanthin (596 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- Bacteriopheophytin a (889 Da, Ligand)
- Reaction center protein l chain (30 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Water (18 Da, Ligand)
- Fe (iii) ion (55 Da, Ligand)