Structure of rat beta-arrestin 1 by fiducial-assisted cryo-EM
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 9dng
Q-score: 0.431
Kahsai AW, Pakharukova N, Kwon HY, Shah KS, Liang-Lin JG, Del Real CT, Shim PJ, Lee MA, Ngo VA, Shreiber BN, Liu S, Schwalb AM, Espinoza EF, Thomas BN, Kunzle CA, Smith JS, Wang J, Kim J, Zhang X, Rockman HA, Thomsen ARB, Rein LAM, Shi L, Ahn S, Masoudi A, Lefkowitz RJ
bioRxiv (2024) [ DOI: doi:10.1101/2024.12.27.630464 Pubmed: 39763753 ]
- Beta-arrestin-1,soluble cytochrome b562 (55 kDa, Protein from Rattus norvegicus)
- Anti-bril fab light chain (23 kDa, Protein from Homo sapiens)
- Anti-bril fab heavy chain (23 kDa, Protein from Homo sapiens)
- Beta-arrestin 1 with insertion of soluble cytochrome b562 bound to anti-bril fab (Complex from Rattus norvegicus)
Structure of rat beta-arrestin 1 bound to allosteric inhibitor
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 9dnm
Q-score: 0.466
Kahsai AW, Pakharukova N, Kwon HY, Shah KS, Liang-Lin JG, Del Real CT, Shim PJ, Lee MA, Ngo VA, Shreiber BN, Liu S, Schwalb AM, Espinoza EF, Thomas BN, Kunzle CA, Smith JS, Wang J, Kim J, Zhang X, Rockman HA, Thomsen ARB, Rein LAM, Shi L, Ahn S, Masoudi A, Lefkowitz RJ
bioRxiv (2024) [ DOI: doi:10.1101/2024.12.27.630464 Pubmed: 39763753 ]
- (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14r,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one (366 Da, Ligand)
- Beta-arrestin-1,soluble cytochrome b562 (55 kDa, Protein from Rattus norvegicus)
- Beta-arrestin 1 with insertion of soluble cytochrome b562 bound to anti-bril fab (Complex from Rattus norvegicus)
- Anti-bril fab light chain (23 kDa, Protein from Homo sapiens)
- Anti-bril fab heavy chain (23 kDa, Protein from Homo sapiens)
Stabilized beta-arrestin 1-V2T subcomplex of a GPCR-G protein-beta-arrestin mega-complex
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6ni2
Q-score: 0.348
Nguyen AH, Thomsen ARB, Cahill 3rd TJ, Huang R, Huang LY, Marcink T, Clarke OB, Heissel S, Masoudi A, Ben-Hail D, Samaan F, Dandey VP, Tan YZ, Hong C, Mahoney JP, Triest S, Little 4th J, Chen X, Sunahara R, Steyaert J, Molina H, Yu Z, des Georges A, Lefkowitz RJ
Nat Struct Mol Biol (2019) 26 pp. 1123-1131 [ DOI: doi:10.1038/s41594-019-0330-y Pubmed: 31740855 ]
- Vasopressin 2 tail (Complex from Homo sapiens)
- Stabilized beta-arrestin 1-v2t subcomplex of a gpcr-g protein-beta-arrestin mega-complex (Complex)
- Fab30 light chain (23 kDa, Protein from synthetic construct)
- Nanobody 32 (13 kDa, Protein from Lama glama)
- Beta-arrestin-1 (44 kDa, Protein from Bos taurus)
- Fab30 heavy chain (25 kDa, Protein from synthetic construct)
- Vasopressin v2 receptor (3 kDa, Protein from Homo sapiens)
- Fab30 (Complex from synthetic construct)
- Nanobody 32 (Complex from Lama glama)
- Beta-arrestin-1 (Complex from Bos taurus)
B2V2R-Gs protein subcomplex of a GPCR-G protein-beta-arrestin mega-complex
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6ni3
Q-score: 0.39
Nguyen AH, Thomsen ARB, Cahill 3rd TJ, Huang R, Huang LY, Marcink T, Clarke OB, Heissel S, Masoudi A, Ben-Hail D, Samaan F, Dandey VP, Tan YZ, Hong C, Mahoney JP, Triest S, Little 4th J, Chen X, Sunahara R, Steyaert J, Molina H, Yu Z, des Georges A, Lefkowitz RJ
Nat Struct Mol Biol (2019) 26 pp. 1123-1131 [ DOI: doi:10.1038/s41594-019-0330-y Pubmed: 31740855 ]
- B2v2r-gs protein subcomplex of a gpcr-g protein-beta-arrestin mega-complex (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- B2v2r-gs protein subcomplex (Complex from Homo sapiens)
- Endolysin,beta-2 adrenergic receptor chimera (57 kDa, Protein from Homo sapiens)
- 8-[(1r)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2h-1,4-benzoxazin-3(4h)-one (370 Da, Ligand)
- Nanobody 35 (15 kDa, Protein from Lama glama)
Structure of a GPCR-G protein-beta-arrestin mega-complex
Nguyen AH, Thomsen ARB, Huang R, Huang LY, Marcink T, Clarke OB, Heissel S, Masoudi A, Ben-Hail D, Samaan F, Dandey VP, Tan YZ, Hong C, Mahoney JP, Triest S, Chen X, Sunahara R, Steyaert J, Molina H, Yu Z, des Georges A, Lefkowitz RJ
Nat Struct Mol Biol (2019) 26 pp. 1123-1131 [ DOI: doi:10.1038/s41594-019-0330-y Pubmed: 31740855 ]