Cryo-EM structure of the E. coli BrxX methyltransferase in complex with DNA
Drobiazko A, Adams MC, Skutel M, Potekhina K, Kotovskaya O, Trofimova A, Matlashov M, Yatselenko D, Maxwell KL, Blower TR, Severinov K, Ghilarov D, Isaev A
Nat Commun (2025) 16 pp. 1825-1825 [ Pubmed: 39979294 DOI: doi:10.1038/s41467-025-57006-2 ]
- Dna (Complex from Escherichia coli)
- Brxx methyltransferase (Complex from Escherichia coli)
- Dna (26-mer) with brex binding site ggtaag (7 kDa, DNA from Escherichia coli)
- Brxx methyltransferase from e. coli brex phage defense system in complex with dna (Complex)
- Magnesium ion (24 Da, Ligand)
- Dna (26-mer) with brex binding site ggtaag (7 kDa, DNA from Escherichia coli)
- Adenine-specific methyltransferase brxx (138 kDa, Protein from Escherichia coli)
- S-adenosylmethionine (398 Da, Ligand)
Focused map for NTD and MTD domains of the phage immunity methyltransferase protein BrxX.
Drobiazko A, Adams MC, Skutel M, Potekhina K, Kotovskaya O, Trofimova A, Matlashov M, Yatselenko D, Maxwell KL, Blower TR, Severinov K, Ghilarov D, Isaev A
Nat Commun (2025) 16 pp. 1825-1825 [ Pubmed: 39979294 DOI: doi:10.1038/s41467-025-57006-2 ]
Consensus map for the phage immunity methyltransferase protein BrxX
Drobiazko A, Adams MC, Skutel M, Potekhina K, Kotovskaya O, Trofimova A, Matlashov M, Yatselenko D, Maxwell KL, Blower TR, Severinov K, Ghilarov D, Isaev A
Nat Commun (2025) 16 pp. 1825-1825 [ Pubmed: 39979294 DOI: doi:10.1038/s41467-025-57006-2 ]
Cryo-EM structure of the E. coli BrxX methyltransferase in complex with Ocr
Drobiazko A, Adams MC, Skutel M, Potekhina K, Kotovskaya O, Trofimova A, Matlashov M, Yatselenko D, Maxwell KL, Blower TR, Severinov K, Ghilarov D, Isaev A
Nat Commun (2025) 16 pp. 1825-1825 [ Pubmed: 39979294 DOI: doi:10.1038/s41467-025-57006-2 ]
- Brxx methyltransferase (Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Brxx methyltransferase from e. coli brex phage defense system in complex with ocr (Complex)
- Protein ocr (13 kDa, Protein from Escherichia phage T7)
- Ocr (Complex from Escherichia phage T7)
- Adenine-specific methyltransferase brxx (138 kDa, Protein from Escherichia coli)
- S-adenosylmethionine (398 Da, Ligand)
Cryo-EM structure of the Apo E. coli BrxX methyltransferase
Drobiazko A, Adams MC, Skutel M, Potekhina K, Kotovskaya O, Trofimova A, Matlashov M, Yatselenko D, Maxwell KL, Blower TR, Severinov K, Ghilarov D, Isaev A
Nat Commun (2025) 16 pp. 1825-1825 [ Pubmed: 39979294 DOI: doi:10.1038/s41467-025-57006-2 ]
Chowdhury S, Carter J, Rollins MF, Golden SM, Jackson RN, Hoffmann C, Nosaka L, Bondy-Denomy J, Maxwell KL, Davidson AR, Fischer ER, Lander GC, Wiedenheft B
Cell (2017) 169 pp. 47-57.e11 [ DOI: doi:10.1016/j.cell.2017.03.012 Pubmed: 28340349 ]
- Anti-crispr protein 30 (10 kDa, Protein from Pseudomonas phage D3112)
- Crispr-associated protein csy2 (36 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Crispr rna (60-mer) (19 kDa, RNA from Pseudomonas aeruginosa UCBPP-PA14)
- Anti-crisprs acrf1 and acrf2 bound p. aeruginosa crrna-guided crispr surveillance(csy)complex. (450 kDa, Complex from Pseudomonas aeruginosa UCBPP-PA14)
- Crispr-associated protein csy3 (37 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Crispr-associated protein csy1 (49 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Crispr-associated endonuclease cas6/csy4 (21 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Anti-crispr protein acr30-35 (8 kDa, Protein from Pseudomonas phage JBD30)