Electron Microscopy Data Bank

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P. chlororaphis 70S ribosome in situ subtomogram average

Release date: July 27, 2022
Resolution: 10.2 Å
EM Method: Subtomogram averaging

Laughlin TG, Deep A, Prichard AM, Seitz C, Gu Y, Enustun E, Suslov S, Khanna K, Birkholz EA, Armbruster E, McCammon JA, Amaro RE, Pogliano J, Corbett KD, Villa E

Nature (2022) 608 pp. 429-435 [ Pubmed: 35922510 DOI: doi:10.1038/s41586-022-05013-4 ]

Sample:

P. chlororaphis 70s ribosome (2 MDa, Complex from Pseudomonas chlororaphis)

EMD-25220

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In situ subtomogram average of the 201phi2-1 phage nucleus major shell protein, chimallin (concave class)

Release date: July 27, 2022
Resolution: 20.0 Å
EM Method: Subtomogram averaging

Laughlin TG, Deep A, Prichard AM, Seitz C, Gu Y, Enustun E, Suslov S, Khanna K, Birkholz EA, Armbruster E, McCammon JA, Amaro RE, Pogliano J, Corbett KD, Villa E

Nature (2022) 608 pp. 429-435 [ Pubmed: 35922510 DOI: doi:10.1038/s41586-022-05013-4 ]

Sample:

201phi2-1 jumbo phage major shell protein, chimallin (concave class) (Cellular component from Pseudomonas phage 201phi2-1)

EMD-25221

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In situ consensus subtomogram average of the 201phi2-1 chimallin

Release date: July 27, 2022
Resolution: 24.0 Å
EM Method: Subtomogram averaging

Laughlin TG, Deep A, Prichard AM, Seitz C, Gu Y, Enustun E, Suslov S, Khanna K, Birkholz EA, Armbruster E, McCammon JA, Amaro RE, Pogliano J, Corbett KD, Villa E

Nature (2022) 608 pp. 429-435 [ Pubmed: 35922510 DOI: doi:10.1038/s41586-022-05013-4 ]

Sample:

201phi2-1 jumbo phage major shell protein, chimallin (Cellular component from Pseudomonas phage 201phi2-1)

EMD-25222

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In situ subtomogram average of 201phi2-1 phage nucleus major shell protein, chimallin (intermediate/flat class)

Release date: July 27, 2022
Resolution: 18.0 Å
EM Method: Subtomogram averaging

Laughlin TG, Deep A, Prichard AM, Seitz C, Gu Y, Enustun E, Suslov S, Khanna K, Birkholz EA, Armbruster E, McCammon JA, Amaro RE, Pogliano J, Corbett KD, Villa E

Nature (2022) 608 pp. 429-435 [ Pubmed: 35922510 DOI: doi:10.1038/s41586-022-05013-4 ]

Sample:

201phi2-1 jumbo phage major shell protein, chimallin (Cellular component from Pseudomonas phage 201phi2-1)

EMD-25223

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In situ subtomogram average of the 201phi2-1 phage nucleus major shell protein, chimallin (convex class)

Release date: July 27, 2022
Resolution: 23.0 Å
EM Method: Subtomogram averaging

Laughlin TG, Deep A, Prichard AM, Seitz C, Gu Y, Enustun E, Suslov S, Khanna K, Birkholz EA, Armbruster E, McCammon JA, Amaro RE, Pogliano J, Corbett KD, Villa E

Nature (2022) 608 pp. 429-435 [ Pubmed: 35922510 DOI: doi:10.1038/s41586-022-05013-4 ]

Sample:

201phi2-1 jumbo phage major shell protein, chimallin (Cellular component from Pseudomonas phage 201phi2-1)

EMD-25229

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In situ subtomogram average of the Goslar major phage nucleus shell protein, chimallin (consensus class)

Release date: July 27, 2022
Resolution: 27.0 Å
EM Method: Subtomogram averaging

Laughlin TG, Deep A, Prichard AM, Seitz C, Gu Y, Enustun E, Suslov S, Khanna K, Birkholz EA, Armbruster E, McCammon JA, Amaro RE, Pogliano J, Corbett KD, Villa E

Nature (2022) 608 pp. 429-435 [ Pubmed: 35922510 DOI: doi:10.1038/s41586-022-05013-4 ]

Sample:

Goslar chimallin in the context of the phage nucleus (Complex from Goslarvirus)

EMD-25262

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In situ subtomogram average of Goslar phage nucleus major shell protein, chimallin (concave class)

Release date: July 27, 2022
Resolution: 20.0 Å
EM Method: Subtomogram averaging

Laughlin TG, Deep A, Prichard AM, Seitz C, Gu Y, Enustun E, Suslov S, Khanna K, Birkholz EA, Armbruster E, McCammon JA, Amaro RE, Pogliano J, Corbett KD, Villa E

Nature (2022) 608 pp. 429-435 [ Pubmed: 35922510 DOI: doi:10.1038/s41586-022-05013-4 ]

Sample:

Chimallin sheet, concave class (Complex from Goslarvirus)

EMD-25358

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In situ subtomogram average of the major Goslar phage nucleus shell protein, chimallin (convex class)

Release date: July 27, 2022
Resolution: 25.0 Å
EM Method: Subtomogram averaging

Laughlin TG, Deep A, Prichard AM, Seitz C, Gu Y, Enustun E, Suslov S, Khanna K, Birkholz EA, Armbruster E, McCammon JA, Amaro RE, Pogliano J, Corbett KD, Villa E

Nature (2022) 608 pp. 429-435 [ Pubmed: 35922510 DOI: doi:10.1038/s41586-022-05013-4 ]

Sample:

Goslar chimallin, convex class. (Cell from Goslarvirus)

EMD-25359

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In situ subtomogram average of the APEC2248 70S ribosome

Release date: July 27, 2022
Resolution: 8.53 Å
EM Method: Subtomogram averaging

Laughlin TG, Deep A, Prichard AM, Seitz C, Gu Y, Enustun E, Suslov S, Khanna K, Birkholz EA, Armbruster E, McCammon JA, Amaro RE, Pogliano J, Corbett KD, Villa E

Nature (2022) 608 pp. 429-435 [ Pubmed: 35922510 DOI: doi:10.1038/s41586-022-05013-4 ]

Sample:

Apec 2248 70s ribosoome (Complex from Escherichia coli)

EMD-25360

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In situ subtomogram average of the APEC2248 50S ribosome

Release date: July 27, 2022
Resolution: 12.0 Å
EM Method: Subtomogram averaging

Laughlin TG, Deep A, Prichard AM, Seitz C, Gu Y, Enustun E, Suslov S, Khanna K, Birkholz EA, Armbruster E, McCammon JA, Amaro RE, Pogliano J, Corbett KD, Villa E

Nature (2022) 608 pp. 429-435 [ Pubmed: 35922510 DOI: doi:10.1038/s41586-022-05013-4 ]

Sample:

Apec 2248 70s ribosoome (Complex from Escherichia coli)

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