Molybdenum storage protein under turnover conditions
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6rkd
Q-score: 0.546
Brunle S, Eisinger ML, Poppe J, Mills DJ, Langer JD, Vonck J, Ermler U
PNAS (2019) [ Pubmed: 31811022 DOI: doi:10.1073/pnas.1913031116 ]
- Mo(vi)(=o)(oh)2 cluster (145 Da, Ligand)
- Oxidanyl-[[2,2,4,4,4-pentakis($l^{1}-oxidanyl)-1-(oxidanylmolybdenio)-1$l^{3},3-dioxa-2$l^{5},4$l^{5}-dimolybdacyclobut-2-yl]oxy]molybdenum (545 Da, Ligand)
- Bis(mu4-oxo)-tetrakis(mu3-oxo)-hexakis(mu2-oxo)-hexadecaoxo-octamolybdenum (vi) (1 kDa, Ligand)
- Molybdenum storage protein subunit alpha (29 kDa, Protein from Azotobacter vinelandii (strain DJ / ATCC BAA-1303))
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Molybdenum storage protein subunit beta (28 kDa, Protein from Azotobacter vinelandii (strain DJ / ATCC BAA-1303))
- Dimer of a3b3 heterohexamer of molybdenum storage protein (380 kDa, Complex from Azotobacter vinelandii DJ)
- Molybdate ion (159 Da, Ligand)
Cryo-EM structure of the E. coli cytochrome bd-I oxidase at 2.68 A resolution
Resolution: 2.68 Å
EM Method: Single-particle
Fitted PDBs: 6rko
Q-score: 0.665
Safarian S, Hahn A, Mills DJ, Radloff M, Eisinger ML, Nikolaev A, Meier-Credo J, Melin F, Miyoshi H, Gennis RB, Sakamoto J, Langer JD, Hellwig P, Kuhlbrandt W, Michel H
Science (2019) 366 pp. 100-104 [ Pubmed: 31604309 DOI: doi:10.1126/science.aay0967 ]
- Ubiquinone-8 (727 Da, Ligand)
- Cis-heme d hydroxychlorin gamma-spirolactone (632 Da, Ligand)
- Uncharacterized protein ynhf (2 kDa, Protein from Escherichia coli (strain K12))
- Cytochrome bd-i oxidase from e. coli (107 kDa, Complex from Escherichia coli K-12)
- Cytochrome bd-i ubiquinol oxidase subunit 1 (58 kDa, Protein from Escherichia coli (strain K12))
- Cytochrome bd-i ubiquinol oxidase subunit x (4 kDa, Protein from Escherichia coli (strain K12))
- Cytochrome bd-i ubiquinol oxidase subunit 2 (42 kDa, Protein from Escherichia coli (strain K12))
- Heme b/c (618 Da, Ligand)
- Water (18 Da, Ligand)
- Oxygen molecule (31 Da, Ligand)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
CryoEM structure of Polytomella F-ATP synthase, Full dimer, composite map
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6rd4
Q-score: 0.557
Murphy BJ, Klusch N, Langer J, Mills DJ, Yildiz O, Kuhlbrandt W
Science (2019) 364 [ Pubmed: 31221832 DOI: doi:10.1126/science.aaw9128 ]
- Mitochondrial atp synthase subunit c (12 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase associated protein asa7 (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Epsilon: polytomella f-atp synthase epsilon subunit (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Mitochondrial f-atp synthase dimer from polytomella sp. pringsheim 198.80 (1 MDa, Complex from Polytomella sp. Pringsheim 198.80)
- Mitochondrial f1f0 atp synthase associated 14 kda protein (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase associated protein asa4 (31 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase subunit alpha (60 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial f1f0 atp synthase associated 32 kda protein (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Atp synthase gamma chain, mitochondrial (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit 6 (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Water (18 Da, Ligand)
- Mitochondrial atp synthase subunit delta (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Asa-10: polytomella f-atp synthase associated subunit 10 (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase associated protein asa1 (68 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa9 (11 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase subunit beta (61 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Magnesium ion (24 Da, Ligand)
- Mitochondrial atp synthase subunit oscp (25 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Zinc ion (65 Da, Ligand)
- Mitochondrial atp synthase subunit asa2 (44 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa8 (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa6 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Resolution: 2.69 Å
EM Method: Single-particle
Fitted PDBs: 6rd5
Q-score: 0.601
Murphy BJ, Klusch N, Langer J, Mills DJ, Yildiz O, Kuhlbrandt W
Science (2019) 364 [ Pubmed: 31221832 DOI: doi:10.1126/science.aaw9128 ]
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Polytomella f-atp synthase, focussed refinement of fo and peripheral stalk, c2 symmetry (Complex from Polytomella sp. Pringsheim 198.80)
- Asa-10: polytomella f-atp synthase associated subunit 10 (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Water (18 Da, Ligand)
- Mitochondrial f1f0 atp synthase associated 14 kda protein (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- (1s)-2-{[(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (720 Da, Ligand)
- Atp synthase associated protein asa1 (68 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit 6 (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa9 (11 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial f1f0 atp synthase associated 32 kda protein (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa8 (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa6 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
CryoEM structure of Polytomella F-ATP synthase, focussed refinement of upper peripheral stalk
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 6rd6
Q-score: 0.587
Murphy BJ, Klusch N, Langer J, Mills DJ, Yildiz O, Kuhlbrandt W
Science (2019) 364 [ Pubmed: 31221832 DOI: doi:10.1126/science.aaw9128 ]
- Asa-2: polytomella f-atp synthase associated subunit 2 (44 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Dimeric mitochondrial f-type atp synthase from polytomella sp. pringsheim 198.80 (Complex from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase associated protein asa7 (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase associated protein asa4 (31 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase subunit alpha (60 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit oscp (25 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Water (18 Da, Ligand)
Resolution: 2.73 Å
EM Method: Single-particle
Fitted PDBs: 6rd7
Q-score: 0.571
Murphy BJ, Klusch N, Langer J, Mills DJ, Yildiz O, Kuhlbrandt W
Science (2019) 364 [ Pubmed: 31221832 DOI: doi:10.1126/science.aaw9128 ]
- Mitochondrial atp synthase subunit c (12 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Zinc ion (65 Da, Ligand)
- Water (18 Da, Ligand)
- Asa-10: polytomella f-atp synthase associated subunit 10 (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial f1f0 atp synthase associated 14 kda protein (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase associated protein asa1 (68 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit 6 (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial f1f0 atp synthase associated 32 kda protein (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Polytomella f-atp synthase (Complex from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa8 (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa6 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Asa-9: polytomella f-atp synthase associated subunit 9 (11 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 6rd8
Q-score: 0.548
Murphy BJ, Klusch N, Langer J, Mills DJ, Yildiz O, Kuhlbrandt W
Science (2019) 364 [ Pubmed: 31221832 DOI: doi:10.1126/science.aaw9128 ]
- Mitochondrial atp synthase subunit c (12 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Zinc ion (65 Da, Ligand)
- Water (18 Da, Ligand)
- Asa-10: polytomella f-atp synthase associated subunit 10 (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial f1f0 atp synthase associated 14 kda protein (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase associated protein asa1 (68 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit 6 (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa8 (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial f1f0 atp synthase associated 32 kda protein (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Polytomella f-atp synthase subunits asa1, asa3, asa5, asa6, asa8, asa9, asa10, c-ring and a-subunit. (Complex from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa6 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Asa-9: polytomella f-atp synthase associated subunit 9 (11 kDa, Protein from Polytomella sp. Pringsheim 198.80)
CryoEM structure of Polytomella F-ATP synthase, Primary rotary state 1, composite map
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6rd9
Q-score: 0.57
Murphy BJ, Klusch N, Langer J, Mills DJ, Yildiz O, Kuhlbrandt W
Science (2019) 364 [ Pubmed: 31221832 DOI: doi:10.1126/science.aaw9128 ]
- Asa-2: polytomella f-atp synthase associated subunit 2 (44 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit c (12 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase associated protein asa7 (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Epsilon: polytomella f-atp synthase epsilon subunit (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Mitochondrial f-atp synthase dimer from polytomella sp. pringsheim 198.80 (1 MDa, Complex from Polytomella sp. Pringsheim 198.80)
- Mitochondrial f1f0 atp synthase associated 14 kda protein (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase associated protein asa4 (31 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase subunit alpha (60 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial f1f0 atp synthase associated 32 kda protein (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Atp synthase gamma chain, mitochondrial (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit 6 (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Water (18 Da, Ligand)
- Mitochondrial atp synthase subunit delta (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Asa-10: polytomella f-atp synthase associated subunit 10 (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase associated protein asa1 (68 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase subunit beta (61 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Magnesium ion (24 Da, Ligand)
- Mitochondrial atp synthase subunit oscp (25 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Zinc ion (65 Da, Ligand)
- Mitochondrial atp synthase subunit asa8 (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa6 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Asa-9: polytomella f-atp synthase associated subunit 9 (11 kDa, Protein from Polytomella sp. Pringsheim 198.80)
CryoEM structure of Polytomella F-ATP synthase, Primary rotary state 1, monomer-masked refinement
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 6rda
Q-score: 0.56
Murphy BJ, Klusch N, Langer J, Mills DJ, Yildiz O, Kuhlbrandt W
Science (2019) 364 [ Pubmed: 31221832 DOI: doi:10.1126/science.aaw9128 ]
- Asa-2: polytomella f-atp synthase associated subunit 2 (44 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Zinc ion (65 Da, Ligand)
- Water (18 Da, Ligand)
- Asa-10: polytomella f-atp synthase associated subunit 10 (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial f1f0 atp synthase associated 14 kda protein (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase associated protein asa7 (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase associated protein asa1 (68 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase associated protein asa4 (31 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit 6 (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase subunit alpha (60 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial f1f0 atp synthase associated 32 kda protein (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Polytomella f-atp synthase (Complex from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa8 (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit oscp (25 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa6 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Asa-9: polytomella f-atp synthase associated subunit 9 (11 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 6rdb
Q-score: 0.558
Murphy BJ, Klusch N, Langer J, Mills DJ, Yildiz O, Kuhlbrandt W
Science (2019) 364 [ Pubmed: 31221832 DOI: doi:10.1126/science.aaw9128 ]
- Mitochondrial atp synthase subunit delta (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Mitochondrial atp synthase subunit c (12 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase subunit alpha (60 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Atp synthase subunit beta (61 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Magnesium ion (24 Da, Ligand)
- Atp synthase gamma chain, mitochondrial (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Polytomella f-atp synthase (Complex from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit oscp (25 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Water (18 Da, Ligand)
- Epsilon: polytomella f-atp synthase epsilon subunit (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)