Mycobacterium tuberculosis RNAP paused elongation complex with NusG transcription factor
Resolution: 2.94 Å
EM Method: Single-particle
Fitted PDBs: 8e74
Q-score: 0.543
Delbeau M, Omollo EO, Froom R, Koh S, Mooney RA, Lilic M, Brewer JJ, Rock J, Darst SA, Campbell EA, Landick R
Mol Cell (2023) 83 pp. 1474-1488.e8 [ DOI: doi:10.1016/j.molcel.2023.04.007 Pubmed: 37116494 ]
- Mycobacterium tuberculosis rnap paused elongation complex with nusg transcription factor (Complex from Mycobacterium tuberculosis)
- Zinc ion (65 Da, Ligand)
- Transcription termination/antitermination protein nusg (25 kDa, Protein from Mycobacterium tuberculosis)
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Rna (42-mer) (13 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit beta' (147 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta (129 kDa, Protein from Mycobacterium tuberculosis)
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
Resolution: 3.71 Å
EM Method: Single-particle
Fitted PDBs: 8e79
Q-score: 0.449
Delbeau M, Omollo EO, Froom R, Koh S, Mooney RA, Lilic M, Brewer JJ, Rock J, Darst SA, Campbell EA, Landick R
Mol Cell (2023) 83 pp. 1474-1488.e8 [ DOI: doi:10.1016/j.molcel.2023.04.007 Pubmed: 37116494 ]
- Zinc ion (65 Da, Ligand)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Rna (42-mer) (13 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit beta' (147 kDa, Protein from Mycobacterium tuberculosis)
- Mycobacterium tuberculosis rnap paused elongation complex with escherchia coli nusg transcription factor (Complex from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta (129 kDa, Protein from Mycobacterium tuberculosis)
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
Mycobacterium tuberculosis RNAP elongation complex with NusG transcription factor
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 8e82
Q-score: 0.447
Delbeau M, Omollo EO, Froom R, Koh S, Mooney RA, Lilic M, Brewer JJ, Rock J, Darst SA, Campbell EA, Landick R
Mol Cell (2023) 83 pp. 1474-1488.e8 [ DOI: doi:10.1016/j.molcel.2023.04.007 Pubmed: 37116494 ]
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Transcription termination/antitermination protein nusg (25 kDa, Protein from Mycobacterium tuberculosis)
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Mycobacterium tuberculosis rnap elongation complex with nusg transcription factor (Complex from Mycobacterium tuberculosis)
- Magnesium ion (24 Da, Ligand)
- Rna (5'-r(p*cp*gp*gp*ap*gp*ap*gp*gp*up*a)-3') (6 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit beta' (147 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta (129 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
Mycobacterium tuberculosis RNAP paused elongation complex
Resolution: 3.13 Å
EM Method: Single-particle
Fitted PDBs: 8e8m
Q-score: 0.496
Delbeau M, Omollo EO, Froom R, Koh S, Mooney RA, Lilic M, Brewer JJ, Rock J, Darst SA, Campbell EA, Landick R
Mol Cell (2023) 83 pp. 1474-1488.e8 [ DOI: doi:10.1016/j.molcel.2023.04.007 Pubmed: 37116494 ]
- Zinc ion (65 Da, Ligand)
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Rna (42-mer) (13 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit beta' (147 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta (129 kDa, Protein from Mycobacterium tuberculosis)
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Mycobacterium tuberculosis rnap paused elongation complex (Complex from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
Mycobacterium tuberculosis RNAP elongation complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8e95
Q-score: 0.497
Delbeau M, Omollo EO, Froom R, Koh S, Mooney RA, Lilic M, Brewer JJ, Rock J, Darst SA, Campbell EA, Landick R
Mol Cell (2023) 83 pp. 1474-1488.e8 [ DOI: doi:10.1016/j.molcel.2023.04.007 Pubmed: 37116494 ]
- Zinc ion (65 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Rna (5'-r(p*cp*gp*gp*ap*gp*ap*gp*gp*up*a)-3') (6 kDa, RNA from synthetic construct)
- Dna (33-mer) (16 kDa, DNA from synthetic construct)
- Mycobacterium tuberculosis rnap elongation complex (Complex from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta' (147 kDa, Protein from Mycobacterium tuberculosis)
- Dna (25-mer) (16 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta (129 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
Cryo-EM structure of Escherichia coli paused complex of transcription termination (TTC-pause)
Resolution: 3.58 Å
EM Method: Single-particle
Fitted PDBs: 7yp9
Q-score: 0.345
You L, Omollo EO, Yu C, Mooney RA, Shi J, Shen L, Wu X, Wen A, He D, Zeng Y, Feng Y, Landick R, Zhang Y
Nature (2023) 613 pp. 783-789 [ DOI: doi:10.1038/s41586-022-05604-1 Pubmed: 36631609 ]
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli K-12)
- Rna (5'-r(*gp*cp*gp*up*cp*gp*cp*ap*gp*gp*cp*cp*up*up*up*up*up*ap*up*u)-3') (6 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase (Complex)
- Dna (31-mer) (9 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Dna/rna (Complex)
- Dna (31-mer) (9 kDa, DNA from synthetic construct)
- E. coli paused complex of transcription termination (400 kDa, Complex from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 7ypa
Q-score: 0.509
You L, Omollo EO, Yu C, Mooney RA, Shi J, Shen L, Wu X, Wen A, He D, Zeng Y, Feng Y, Landick R, Zhang Y
Nature (2023) 613 pp. 783-789 [ DOI: doi:10.1038/s41586-022-05604-1 Pubmed: 36631609 ]
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli K-12)
- Rna (5'-r(*gp*cp*gp*up*cp*gp*cp*ap*gp*gp*cp*cp*up*up*up*up*up*ap*up*u)-3') (6 kDa, RNA from synthetic construct)
- Rna (5'-r(*cp*cp*up*gp*cp*gp*ap*cp*gp*c)-3') (3 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase (Complex)
- Dna (31-mer) (9 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Dna/rna (Complex)
- Dna (31-mer) (9 kDa, DNA from synthetic construct)
- E. coli hairpin-nucleation complex of transcription termination (400 kDa, Complex from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
Cryo-EM structure of Escherichia coli release complex of transcription termination (TTC-release)
Resolution: 3.48 Å
EM Method: Single-particle
Fitted PDBs: 7ypb
Q-score: 0.399
You L, Omollo EO, Yu C, Mooney RA, Shi J, Shen L, Wu X, Wen A, He D, Zeng Y, Feng Y, Landick R, Zhang Y
Nature (2023) 613 pp. 783-789 [ DOI: doi:10.1038/s41586-022-05604-1 Pubmed: 36631609 ]
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli K-12)
- Rna (5'-r(*gp*gp*cp*cp*up*gp*cp*gp*ap*cp*gp*a)-3') (3 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase (Complex)
- Dna (31-mer) (9 kDa, DNA from synthetic construct)
- Dna (31-mer) (9 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- E. coli release complex of transcription termination (400 kDa, Complex from Escherichia coli K-12)
- Dna/rna (Complex)
- Rna (5'-r(*gp*cp*gp*up*cp*gp*cp*ap*gp*gp*cp*cp*up*up*up*up*up*ap*up*u)-3') (6 kDa, RNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
CryoEM of Mycobacterium tuberculosis WT RNAP holoenzyme/RbpA bound to the Fdx and upstream fork DNA
Boyaci H, Chen J, Lilic M, Palka M, Mooney RA, Landick R, Darst SA, Campbell EA
Elife (2018) 7 [ Pubmed: 29480804 DOI: 10.7554/elife.34823 ]
Image sets:
CryoEM of Mycobacterium tuberculosis WT RNAP holoenzyme/RbpA/Fdx
Boyaci H, Chen J, Lilic M, Palka M, Mooney RA, Landick R, Darst SA, Campbell EA
Elife (2018) 7 pp. 1-19 [ Pubmed: 29480804 DOI: 10.7554/elife.34823 ]
Image sets: