Cryo-EM structure of Escherichia coli paused complex of transcription termination (TTC-pause)
Resolution: 3.58 Å
EM Method: Single-particle
Fitted PDBs: 7yp9
Q-score: 0.345
You L, Omollo EO, Yu C, Mooney RA, Shi J, Shen L, Wu X, Wen A, He D, Zeng Y, Feng Y, Landick R, Zhang Y
Nature (2023) 613 pp. 783-789 [ DOI: doi:10.1038/s41586-022-05604-1 Pubmed: 36631609 ]
- Magnesium ion (24 Da, Ligand)
- Dna (31-mer) (9 kDa, DNA from synthetic construct)
- Rna (5'-r(*gp*cp*gp*up*cp*gp*cp*ap*gp*gp*cp*cp*up*up*up*up*up*ap*up*u)-3') (6 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- E. coli paused complex of transcription termination (400 kDa, Complex from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
- Dna/rna (Complex)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase (Complex)
- Dna (31-mer) (9 kDa, DNA from synthetic construct)
Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 7ypa
Q-score: 0.509
You L, Omollo EO, Yu C, Mooney RA, Shi J, Shen L, Wu X, Wen A, He D, Zeng Y, Feng Y, Landick R, Zhang Y
Nature (2023) 613 pp. 783-789 [ DOI: doi:10.1038/s41586-022-05604-1 Pubmed: 36631609 ]
- Magnesium ion (24 Da, Ligand)
- Dna (31-mer) (9 kDa, DNA from synthetic construct)
- Rna (5'-r(*gp*cp*gp*up*cp*gp*cp*ap*gp*gp*cp*cp*up*up*up*up*up*ap*up*u)-3') (6 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Rna (5'-r(*cp*cp*up*gp*cp*gp*ap*cp*gp*c)-3') (3 kDa, RNA from synthetic construct)
- E. coli hairpin-nucleation complex of transcription termination (400 kDa, Complex from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
- Dna/rna (Complex)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase (Complex)
- Dna (31-mer) (9 kDa, DNA from synthetic construct)
Cryo-EM structure of Escherichia coli release complex of transcription termination (TTC-release)
Resolution: 3.48 Å
EM Method: Single-particle
Fitted PDBs: 7ypb
Q-score: 0.399
You L, Omollo EO, Yu C, Mooney RA, Shi J, Shen L, Wu X, Wen A, He D, Zeng Y, Feng Y, Landick R, Zhang Y
Nature (2023) 613 pp. 783-789 [ DOI: doi:10.1038/s41586-022-05604-1 Pubmed: 36631609 ]
- Dna (31-mer) (9 kDa, DNA from synthetic construct)
- E. coli release complex of transcription termination (400 kDa, Complex from Escherichia coli K-12)
- Rna (5'-r(*gp*gp*cp*cp*up*gp*cp*gp*ap*cp*gp*a)-3') (3 kDa, RNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
- Dna/rna (Complex)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli K-12)
- Rna (5'-r(*gp*cp*gp*up*cp*gp*cp*ap*gp*gp*cp*cp*up*up*up*up*up*ap*up*u)-3') (6 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase (Complex)
- Dna (31-mer) (9 kDa, DNA from synthetic construct)