Resolution: 3.71 Å
EM Method: Single-particle
Fitted PDBs: 8e79
Q-score: 0.449
Delbeau M, Omollo EO, Froom R, Koh S, Mooney RA, Lilic M, Brewer JJ, Rock J, Darst SA, Campbell EA, Landick R
Mol Cell (2023) 83 pp. 1474-1488.e8 [ Pubmed: 37116494 DOI: doi:10.1016/j.molcel.2023.04.007 ]
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
- Mycobacterium tuberculosis rnap paused elongation complex with escherchia coli nusg transcription factor (Complex from Mycobacterium tuberculosis)
- Magnesium ion (24 Da, Ligand)
- Rna (42-mer) (13 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta (129 kDa, Protein from Mycobacterium tuberculosis)
- Zinc ion (65 Da, Ligand)
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta' (147 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
CryoEM structure of E.coli RNA polymerase elongation complex bound with RfaH
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6c6s
Q-score: 0.426
Kang JY, Mooney RA, Nedialkov Y, Saba J, Mishanina TV, Artsimovitch I, Landick R, Darst SA
Cell (2018) 173 pp. 1650-1662.e14 [ Pubmed: 29887376 DOI: doi:10.1016/j.cell.2018.05.017 ]
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Dna (29-mer) (8 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli (strain K12))
- Rna (5'-r(*gp*cp*ap*up*up*cp*ap*ap*ap*gp*cp*cp*gp*ap*gp*ap*gp*gp*up*a)-3') (6 kDa, RNA from Escherichia coli)
- Dna (29-mer) (9 kDa, DNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Transcription antitermination protein rfah (18 kDa, Protein from Escherichia coli (strain K12))
- E.coli rfah-elongation complex (412 kDa, Complex from Escherichia coli)
CryoEM structure of E.coli RNA polymerase elongation complex bound with RfaH
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6c6t
Q-score: 0.462
Kang JY, Mooney RA, Nedialkov Y, Saba J, Mishanina TV, Artsimovitch I, Landick R, Darst SA
Cell (2018) 173 pp. 1650-1662.e14 [ Pubmed: 29887376 DOI: doi:10.1016/j.cell.2018.05.017 ]
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
- Dna (29-mer) (8 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli (strain K12))
- Rna (5'-r(*gp*cp*ap*up*up*cp*ap*ap*ap*gp*cp*cp*gp*ap*gp*ap*gp*gp*up*a)-3') (6 kDa, RNA from Escherichia coli)
- Dna (29-mer) (9 kDa, DNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli (strain K12))
- Transcription antitermination protein rfah (18 kDa, Protein from Escherichia coli (strain K12))
- E.coli rfah-elongation complex (412 kDa, Complex from Escherichia coli)
CryoEM structure of E.coli RNA polymerase elongation complex bound with NusG
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6c6u
Q-score: 0.442
Kang JY, Mooney RA, Nedialkov Y, Saba J, Mishanina TV, Artsimovitch I, Landick R, Darst SA
Cell (2018) 173 pp. 1650-1662.e14 [ Pubmed: 29887376 DOI: doi:10.1016/j.cell.2018.05.017 ]
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase beta' (155 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
- Dna (29-mer) (8 kDa, DNA from Escherichia coli)
- E.coli nusg-elongation complex (414 kDa, Complex from Escherichia coli)
- Rna (5'-r(*gp*cp*ap*up*up*cp*ap*ap*ap*gp*cp*cp*gp*ap*gp*ap*gp*gp*up*a)-3') (6 kDa, RNA from Escherichia coli)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli (strain K12))
- Dna (29-mer) (9 kDa, DNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli (strain K12))
CryoEM structure of E.coli his pause elongation complex
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6asx
Q-score: 0.436
Kang JY, Mishanina TV, Bellecourt MJ, Mooney RA, Darst SA, Landick R
Mol Cell (2018) 69 pp. 802-815.e1 [ DOI: doi:10.1016/j.molcel.2018.01.018 Pubmed: 29499135 ]
- E.coli his pause elongation complex (400 kDa, Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit omega (9 kDa, Protein from Escherichia coli)
- Dna (32-mer) (9 kDa, DNA from Salmonella enterica subsp. enterica serovar Typhimurium)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli (strain K12))
- Rna (29-mer) (9 kDa, RNA from Salmonella enterica subsp. enterica serovar Typhimurium)
- Dna (32-mer) (9 kDa, DNA from Salmonella enterica subsp. enterica serovar Typhimurium)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
CryoEM structure of E.coli his pause elongation complex without pause hairpin
Resolution: 5.5 Å
EM Method: Single-particle
Fitted PDBs: 6bjs
Q-score: 0.229
Kang JY, Mishanina TV, Bellecourt MJ, Mooney RA, Darst SA, Landick R
Mol Cell (2018) 69 pp. 802-815.e1 [ DOI: doi:10.1016/j.molcel.2018.01.018 Pubmed: 29499135 ]
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Dna (32-mer) (9 kDa, DNA from Salmonella enterica subsp. enterica serovar Typhimurium)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli (strain K12))
- Rna (29-mer) (9 kDa, RNA from Salmonella enterica subsp. enterica serovar Typhimurium)
- Dna (32-mer) (9 kDa, DNA from Salmonella enterica subsp. enterica serovar Typhimurium)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli (strain K12))
- E.coli his pause elongation complex without pause hairpin (400 kDa, Complex from Escherichia coli)
30S ribosomal subunit with partial density for ribosomal protein S1
Kohler R, Mooney RA, Mills DJ, Landick R, Cramer P
To Be Published
- Ribosome (Macromolecule from Escherichia coli)
- The sample contained a mixture of in vitro assembled e.coli expressomes and free ribosomes (see related entry emdb 3580 and related publication). this structure shows additional density for ribosomal protein s1 and weak density for the rna polymerase. (Complex from Escherichia coli)