SpCas9 bound to 18 nucleotide complementary DNA substrate in the checkpoint state
Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M
Nature (2022) 609 pp. 191-196 [ DOI: 10.1038/s41586-022-05114-0 Pubmed: 36002571 ]
Image sets:
Human CPSF160-WDR33-CPSF30 complex bound to the PAS AAUAAA motif
Clerici M, Faini M, Muckenfuss LM, Aebersold R, Jinek M
Nat Struct Mol Biol (2018) 25 pp. 135-138 [ Pubmed: 29358758 DOI: 10.1038/s41594-017-0020-6 ]
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SpCas9 bound to 6 nucleotide complementary DNA substrate
Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M
Nature (2022) 609 pp. 191-196 [ DOI: 10.1038/s41586-022-05114-0 Pubmed: 36002571 ]
Image sets:
SpCas9 bound to 12 nucleotide complementary DNA substrate
Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M
Nature (2022) 609 pp. 191-196 [ DOI: 10.1038/s41586-022-05114-0 Pubmed: 36002571 ]
Image sets:
SpCas9 bound to 14 nucleotide complementary DNA substrate
Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M
Nature (2022) 609 pp. 191-196 [ DOI: 10.1038/s41586-022-05114-0 Pubmed: 36002571 ]
Image sets:
SpCas9 bound to 16 nucleotide complementary DNA substrate
Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M
Nature (2022) 609 pp. 191-196 [ DOI: 10.1038/s41586-022-05114-0 Pubmed: 36002571 ]
Image sets:
SpCas9 bound to 18 nucleotide complementary DNA substrate in the catalytic state
Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M
Nature (2022) 609 pp. 191-196 [ DOI: 10.1038/s41586-022-05114-0 Pubmed: 36002571 ]
Image sets:
SpCas9 bound to 10 nucleotide complementary DNA substrate
Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M
Nature (2022) 609 pp. 191-196 [ DOI: 10.1038/s41586-022-05114-0 Pubmed: 36002571 ]
Image sets:
SpCas9 bound to 8 nucleotide complementary DNA substrate
Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M
Nature (2022) 609 pp. 191-196 [ DOI: 10.1038/s41586-022-05114-0 Pubmed: 36002571 ]
Image sets:
SpCas9 bound to 6 nucleotide complementary DNA substrate
Resolution: 3.87 Å
EM Method: Single-particle
Fitted PDBs: 7z4c
Q-score: 0.315
Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M
Nature (2022) 609 pp. 191-196 [ DOI: doi:10.1038/s41586-022-05114-0 Pubmed: 36002571 ]
- Non-target strand of 6 nucleotide complementary dna substrate (9 kDa, DNA from synthetic construct)
- Target strand of 6 nucleotide complementary dna substrate (9 kDa, DNA from synthetic construct)
- Sgrna (33 kDa, RNA from synthetic construct)
- Ternary complex of spcas9-sgrna bound to a 6 nucleotide complementary dna substrate (Complex from Streptococcus pyogenes)
- Crispr-associated endonuclease cas9/csn1 (158 kDa, Protein from Streptococcus pyogenes)