Electron Microscopy Data Bank

Download Map 3D View

Structure of the fungal plasma membrane proton pump Pma1 in its auto-inhibited state - monomer unit

Release date: Nov. 17, 2021
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7nxf
Q-score: 0.439

Heit S, Geurts MMG, Murphy BJ, Corey RA, Mills DJ, Kuhlbrandt W, Bublitz M

Sci Adv (2021) 7 pp. eabj5255-eabj5255 [ DOI: doi:10.1126/sciadv.abj5255 Pubmed: 34757782 ]

Sample:

Adenosine-5'-diphosphate (427 Da, Ligand)
Potassium ion (39 Da, Ligand)
Monomeric subunit of the hexameric fungal plasma membrane proton pump in its auto-inhibited state (Cellular component from Neurospora crassa)
Magnesium ion (24 Da, Ligand)
Plasma membrane atpase (99 kDa, Protein from Neurospora crassa)

[Show more]

EMD-12644

Download Map 3D View

Structure of the fungal plasma membrane proton pump Pma1 in its auto-inhibited state - hexameric assembly

Release date: Nov. 17, 2021
Resolution: 3.26 Å
EM Method: Single-particle
Fitted PDBs: 7ny1
Q-score: 0.357

Heit S, Geurts MMG, Murphy BJ, Corey RA, Mills DJ, Kuhlbrandt W, Bublitz M

Sci Adv (2021) 7 pp. eabj5255-eabj5255 [ DOI: doi:10.1126/sciadv.abj5255 Pubmed: 34757782 ]

Sample:

Adenosine-5'-diphosphate (427 Da, Ligand)
Potassium ion (39 Da, Ligand)
Magnesium ion (24 Da, Ligand)
Plasma membrane atpase (99 kDa, Protein from Neurospora crassa)
Hexameric assembly of the fungal plasma membrane proton pump in its auto-inhibited state (Complex from Neurospora crassa)

[Show more]

EMPIAR-10375

Related EMDB

EMD-4805 EMD-4817 EMD-4845 EMD-4834 EMD-4832 EMD-4812 EMD-4840 EMD-4827 EMD-4808 EMD-4830 EMD-4809 EMD-4831 EMD-4813 EMD-4841 EMD-4819 EMD-4839 EMD-4843 EMD-4850 EMD-4842 EMD-4833 EMD-4837 EMD-4836 EMD-4818 EMD-4824 EMD-4848 EMD-4811 EMD-4854 EMD-4852 EMD-4826 EMD-4807 EMD-4814 EMD-4849 EMD-4810 EMD-4838 EMD-4825 EMD-4815 EMD-4853 EMD-4835 EMD-4821 EMD-4820 EMD-4806 EMD-4857 EMD-4828 EMD-4816 EMD-4851 EMD-4856 EMD-4829 EMD-4847 EMD-4844 EMD-4822 EMD-4823 EMD-4855 EMD-4846

Rotary substates of mitochondrial ATP synthase reveal the basis of flexible F1-Fo coupling

Release date: Dec. 18, 2020
Dataset size: 43.8 TB
Experiment type: EMDB

Murphy BJ, Klusch N, Langer JD, Mills DJ, Yildiz Ö, Kühlbrandt W

Science (2019) 364 [ DOI: 10.1126/science.aaw9128 Pubmed: 31221832 ]

Image sets:

Unaligned frames, gain reference corrected

EMD-4805

Download Map 3D View Raw data

CryoEM structure of Polytomella F-ATP synthase, Full dimer, composite map

Release date: July 3, 2019
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6rd4
Q-score: 0.557

Murphy BJ, Klusch N, Langer J, Mills DJ, Yildiz O, Kuhlbrandt W

Science (2019) 364 [ Pubmed: 31221832 DOI: doi:10.1126/science.aaw9128 ]

Sample:

Mitochondrial atp synthase subunit c (12 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase associated protein asa7 (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Epsilon: polytomella f-atp synthase epsilon subunit (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Adenosine-5'-diphosphate (427 Da, Ligand)
Mitochondrial f-atp synthase dimer from polytomella sp. pringsheim 198.80 (1 MDa, Complex from Polytomella sp. Pringsheim 198.80)
Mitochondrial f1f0 atp synthase associated 14 kda protein (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase associated protein asa4 (31 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Atp synthase subunit alpha (60 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial f1f0 atp synthase associated 32 kda protein (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Adenosine-5'-triphosphate (507 Da, Ligand)
Atp synthase gamma chain, mitochondrial (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase subunit 6 (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Water (18 Da, Ligand)
Mitochondrial atp synthase subunit delta (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Asa-10: polytomella f-atp synthase associated subunit 10 (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Atp synthase associated protein asa1 (68 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase subunit asa9 (11 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Atp synthase subunit beta (61 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Magnesium ion (24 Da, Ligand)
Mitochondrial atp synthase subunit oscp (25 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Zinc ion (65 Da, Ligand)
Mitochondrial atp synthase subunit asa2 (44 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase subunit asa8 (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase subunit asa6 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)

[Show more]

EMD-4806

Download Map 3D View Raw data

CryoEM structure of Polytomella F-ATP synthase, focussed refinement of Fo and peripheral stalk, C2 symmetry

Release date: July 3, 2019
Resolution: 2.69 Å
EM Method: Single-particle
Fitted PDBs: 6rd5
Q-score: 0.601

Murphy BJ, Klusch N, Langer J, Mills DJ, Yildiz O, Kuhlbrandt W

Science (2019) 364 [ Pubmed: 31221832 DOI: doi:10.1126/science.aaw9128 ]

Sample:

Dodecyl-beta-d-maltoside (510 Da, Ligand)
Zinc ion (65 Da, Ligand)
Polytomella f-atp synthase, focussed refinement of fo and peripheral stalk, c2 symmetry (Complex from Polytomella sp. Pringsheim 198.80)
Asa-10: polytomella f-atp synthase associated subunit 10 (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Water (18 Da, Ligand)
Mitochondrial f1f0 atp synthase associated 14 kda protein (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
(1s)-2-{[(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (720 Da, Ligand)
Atp synthase associated protein asa1 (68 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase subunit 6 (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase subunit asa9 (11 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial f1f0 atp synthase associated 32 kda protein (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase subunit asa8 (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase subunit asa6 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)

[Show more]

EMD-4807

Download Map 3D View Raw data

CryoEM structure of Polytomella F-ATP synthase, focussed refinement of upper peripheral stalk

Release date: July 3, 2019
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 6rd6
Q-score: 0.587

Murphy BJ, Klusch N, Langer J, Mills DJ, Yildiz O, Kuhlbrandt W

Science (2019) 364 [ Pubmed: 31221832 DOI: doi:10.1126/science.aaw9128 ]

Sample:

Asa-2: polytomella f-atp synthase associated subunit 2 (44 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Dimeric mitochondrial f-type atp synthase from polytomella sp. pringsheim 198.80 (Complex from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase associated protein asa7 (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase associated protein asa4 (31 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Atp synthase subunit alpha (60 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase subunit oscp (25 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Water (18 Da, Ligand)

[Show more]

EMD-4808

Download Map 3D View Raw data

CryoEM structure of Polytomella F-ATP synthase, c-ring position 1, focussed refinement of Fo and peripheral stalk

Release date: July 3, 2019
Resolution: 2.73 Å
EM Method: Single-particle
Fitted PDBs: 6rd7
Q-score: 0.571

Murphy BJ, Klusch N, Langer J, Mills DJ, Yildiz O, Kuhlbrandt W

Science (2019) 364 [ Pubmed: 31221832 DOI: doi:10.1126/science.aaw9128 ]

Sample:

Mitochondrial atp synthase subunit c (12 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Zinc ion (65 Da, Ligand)
Water (18 Da, Ligand)
Asa-10: polytomella f-atp synthase associated subunit 10 (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial f1f0 atp synthase associated 14 kda protein (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Atp synthase associated protein asa1 (68 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase subunit 6 (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial f1f0 atp synthase associated 32 kda protein (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Polytomella f-atp synthase (Complex from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase subunit asa8 (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase subunit asa6 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Asa-9: polytomella f-atp synthase associated subunit 9 (11 kDa, Protein from Polytomella sp. Pringsheim 198.80)

[Show more]

EMD-4809

Download Map 3D View Raw data

CryoEM structure of Polytomella F-ATP synthase, c-ring position 2, focussed refinement of Fo and peripheral stalk

Release date: July 3, 2019
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 6rd8
Q-score: 0.548

Murphy BJ, Klusch N, Langer J, Mills DJ, Yildiz O, Kuhlbrandt W

Science (2019) 364 [ Pubmed: 31221832 DOI: doi:10.1126/science.aaw9128 ]

Sample:

Mitochondrial atp synthase subunit c (12 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Zinc ion (65 Da, Ligand)
Water (18 Da, Ligand)
Asa-10: polytomella f-atp synthase associated subunit 10 (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial f1f0 atp synthase associated 14 kda protein (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Atp synthase associated protein asa1 (68 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase subunit 6 (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase subunit asa8 (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial f1f0 atp synthase associated 32 kda protein (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Polytomella f-atp synthase subunits asa1, asa3, asa5, asa6, asa8, asa9, asa10, c-ring and a-subunit. (Complex from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase subunit asa6 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Asa-9: polytomella f-atp synthase associated subunit 9 (11 kDa, Protein from Polytomella sp. Pringsheim 198.80)

[Show more]

EMD-4810

Download Map 3D View Raw data

CryoEM structure of Polytomella F-ATP synthase, Primary rotary state 1, composite map

Release date: July 3, 2019
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6rd9
Q-score: 0.57

Murphy BJ, Klusch N, Langer J, Mills DJ, Yildiz O, Kuhlbrandt W

Science (2019) 364 [ Pubmed: 31221832 DOI: doi:10.1126/science.aaw9128 ]

Sample:

Asa-2: polytomella f-atp synthase associated subunit 2 (44 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase subunit c (12 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase associated protein asa7 (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Epsilon: polytomella f-atp synthase epsilon subunit (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Adenosine-5'-diphosphate (427 Da, Ligand)
Mitochondrial f-atp synthase dimer from polytomella sp. pringsheim 198.80 (1 MDa, Complex from Polytomella sp. Pringsheim 198.80)
Mitochondrial f1f0 atp synthase associated 14 kda protein (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase associated protein asa4 (31 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Atp synthase subunit alpha (60 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial f1f0 atp synthase associated 32 kda protein (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Adenosine-5'-triphosphate (507 Da, Ligand)
Atp synthase gamma chain, mitochondrial (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase subunit 6 (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Water (18 Da, Ligand)
Mitochondrial atp synthase subunit delta (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Asa-10: polytomella f-atp synthase associated subunit 10 (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Atp synthase associated protein asa1 (68 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Atp synthase subunit beta (61 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Magnesium ion (24 Da, Ligand)
Mitochondrial atp synthase subunit oscp (25 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Zinc ion (65 Da, Ligand)
Mitochondrial atp synthase subunit asa8 (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase subunit asa6 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Asa-9: polytomella f-atp synthase associated subunit 9 (11 kDa, Protein from Polytomella sp. Pringsheim 198.80)

[Show more]

EMD-4811

Download Map 3D View Raw data

CryoEM structure of Polytomella F-ATP synthase, Primary rotary state 1, monomer-masked refinement

Release date: July 3, 2019
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 6rda
Q-score: 0.56

Murphy BJ, Klusch N, Langer J, Mills DJ, Yildiz O, Kuhlbrandt W

Science (2019) 364 [ Pubmed: 31221832 DOI: doi:10.1126/science.aaw9128 ]

Sample:

Asa-2: polytomella f-atp synthase associated subunit 2 (44 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Zinc ion (65 Da, Ligand)
Water (18 Da, Ligand)
Asa-10: polytomella f-atp synthase associated subunit 10 (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial f1f0 atp synthase associated 14 kda protein (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase associated protein asa7 (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Atp synthase associated protein asa1 (68 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase associated protein asa4 (31 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase subunit 6 (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Atp synthase subunit alpha (60 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial f1f0 atp synthase associated 32 kda protein (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Polytomella f-atp synthase (Complex from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase subunit asa8 (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase subunit oscp (25 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Mitochondrial atp synthase subunit asa6 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Asa-9: polytomella f-atp synthase associated subunit 9 (11 kDa, Protein from Polytomella sp. Pringsheim 198.80)

[Show more]

Filter By

Filter By

Filter By

Number of entries released by year

Download

Download

Filter By