Resolution: 2.71 Å
EM Method: Single-particle
Fitted PDBs: 9fzy
Q-score: 0.673
Yin MD, Lemaire ON, Rosas Jimenez JG, Belhamri M, Shevchenko A, Hummer G, Wagner T, Murphy BJ
Science (2025) 387 pp. 498-504 [ DOI: doi:10.1126/science.adr9672 Pubmed: 39883773 ]
- Carbon monoxide dehydrogenase/acetyl-coa synthase beta subunit (67 kDa, Protein from Clostridium autoethanogenum DSM 10061)
- Acetyl-coa decarbonylase/synthase complex subunit delta (34 kDa, Protein from Clostridium autoethanogenum DSM 10061)
- Corrinoid iron-sulfur protein large subunit (49 kDa, Protein from Clostridium autoethanogenum DSM 10061)
- Co-methylating acetyl-coa synthase (76 kDa, Protein from Clostridium autoethanogenum DSM 10061)
- Iron/sulfur cluster (351 Da, Ligand)
- Cobalamin (1 kDa, Ligand)
- Fe(3)-ni(1)-s(4) cluster (410 Da, Ligand)
- Carbon monoxide dehydrogenase/acetyl-coa synthase in complex with corrinoid/iron-sulfur protein (Complex from Clostridium autoethanogenum DSM 10061)
- Nickel (ii) ion (58 Da, Ligand)
Resolution: 2.65 Å
EM Method: Single-particle
Fitted PDBs: 9fzz
Q-score: 0.675
Yin MD, Lemaire ON, Rosas Jimenez JG, Belhamri M, Shevchenko A, Hummer G, Wagner T, Murphy BJ
Science (2025) 387 pp. 498-504 [ DOI: doi:10.1126/science.adr9672 Pubmed: 39883773 ]
- Carbon monoxide dehydrogenase/acetyl-coa synthase beta subunit (67 kDa, Protein from Clostridium autoethanogenum DSM 10061)
- Acetyl-coa decarbonylase/synthase complex subunit delta (34 kDa, Protein from Clostridium autoethanogenum DSM 10061)
- Corrinoid iron-sulfur protein large subunit (49 kDa, Protein from Clostridium autoethanogenum DSM 10061)
- Co-methylating acetyl-coa synthase (76 kDa, Protein from Clostridium autoethanogenum DSM 10061)
- Iron/sulfur cluster (351 Da, Ligand)
- Fe(3)-ni(1)-s(4) cluster (410 Da, Ligand)
- Cobalamin (1 kDa, Ligand)
- Carbon monoxide dehydrogenase/acetyl-coa synthase in complex with corrinoid/iron-sulfur protein (Complex from Clostridium autoethanogenum DSM 10061)
- Nickel (ii) ion (58 Da, Ligand)
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 9g00
Q-score: 0.663
Yin MD, Lemaire ON, Rosas Jimenez JG, Belhamri M, Shevchenko A, Hummer G, Wagner T, Murphy BJ
Science (2025) 387 pp. 498-504 [ DOI: doi:10.1126/science.adr9672 Pubmed: 39883773 ]
- Carbon monoxide dehydrogenase/acetyl-coa synthase beta subunit (67 kDa, Protein from Clostridium autoethanogenum DSM 10061)
- Acetyl-coa decarbonylase/synthase complex subunit delta (34 kDa, Protein from Clostridium autoethanogenum DSM 10061)
- Corrinoid iron-sulfur protein large subunit (49 kDa, Protein from Clostridium autoethanogenum DSM 10061)
- Co-methylating acetyl-coa synthase (76 kDa, Protein from Clostridium autoethanogenum DSM 10061)
- Iron/sulfur cluster (351 Da, Ligand)
- Cobalamin (1 kDa, Ligand)
- Fe(3)-ni(1)-s(4) cluster (410 Da, Ligand)
- Carbon monoxide dehydrogenase/acetyl-coa synthase in complex with corrinoid/iron-sulfur protein (Complex from Clostridium autoethanogenum DSM 10061)
- Nickel (ii) ion (58 Da, Ligand)
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 9g01
Q-score: 0.706
Yin MD, Lemaire ON, Rosas Jimenez JG, Belhamri M, Shevchenko A, Hummer G, Wagner T, Murphy BJ
Science (2025) 387 pp. 498-504 [ DOI: doi:10.1126/science.adr9672 Pubmed: 39883773 ]
- Carbon monoxide dehydrogenase/acetyl-coa synthase beta subunit (67 kDa, Protein from Clostridium autoethanogenum DSM 10061)
- Carbon monoxide (28 Da, Ligand)
- Carbon monoxide dehydrogenase/acetyl-coa synthase (Complex from Clostridium autoethanogenum DSM 10061)
- Co-methylating acetyl-coa synthase (76 kDa, Protein from Clostridium autoethanogenum DSM 10061)
- Iron/sulfur cluster (351 Da, Ligand)
- Fe(3)-ni(1)-s(4) cluster (410 Da, Ligand)
- Nickel (ii) ion (58 Da, Ligand)
Resolution: 2.1 Å
EM Method: Single-particle
Fitted PDBs: 9g03
Q-score: 0.712
Yin MD, Lemaire ON, Rosas Jimenez JG, Belhamri M, Shevchenko A, Hummer G, Wagner T, Murphy BJ
Science (2025) 387 pp. 498-504 [ DOI: doi:10.1126/science.adr9672 Pubmed: 39883773 ]
- Carbon monoxide dehydrogenase/acetyl-coa synthase beta subunit (67 kDa, Protein from Clostridium autoethanogenum DSM 10061)
- Ferredoxin (5 kDa, Protein from Clostridium autoethanogenum DSM 10061)
- Co-methylating acetyl-coa synthase (76 kDa, Protein from Clostridium autoethanogenum DSM 10061)
- Iron/sulfur cluster (351 Da, Ligand)
- Carbon monoxide dehydrogenase/acetyl-coa synthase in complex with ferredoxin (Complex from Clostridium autoethanogenum DSM 10061)
- Fe(3)-ni(1)-s(4) cluster (410 Da, Ligand)
Structure of the FDX2-bound core ISC complex (proximal conformation)
Resolution: 2.26 Å
EM Method: Single-particle
Fitted PDBs: 8rmc
Q-score: 0.702
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Human fdx2-bound core isc complex (nfs1-isd11-acp1-iscu2-fdx2) (Complex from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Structure of the FDX2-bound core ISC complex (distal conformation)
Resolution: 2.52 Å
EM Method: Single-particle
Fitted PDBs: 8rmd
Q-score: 0.642
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Human fdx2-bound core isc complex (nfs1-isd11-acp1-iscu2-fdx2) (Complex from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Structure of the core ISC complex under turnover conditions (frataxin-bound)
Resolution: 2.49 Å
EM Method: Single-particle
Fitted PDBs: 8rme
Q-score: 0.615
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Frataxin mature form (14 kDa, Protein from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
Structure of the core ISC complex under turnover conditions (FDX2-bound in proximal conformation)
Resolution: 2.33 Å
EM Method: Single-particle
Fitted PDBs: 8rmf
Q-score: 0.633
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
Structure of the core ISC complex under turnover conditions (FDX2-bound in distal conformation)
Resolution: 2.46 Å
EM Method: Single-particle
Fitted PDBs: 8rmg
Q-score: 0.598
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)