Structure of the FDX2-bound core ISC complex (proximal conformation)
Resolution: 2.26 Å
EM Method: Single-particle
Fitted PDBs: 8rmc
Q-score: 0.702
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Fe (ii) ion (55 Da, Ligand)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Human fdx2-bound core isc complex (nfs1-isd11-acp1-iscu2-fdx2) (Complex from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Structure of the FDX2-bound core ISC complex (distal conformation)
Resolution: 2.52 Å
EM Method: Single-particle
Fitted PDBs: 8rmd
Q-score: 0.642
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Fe (ii) ion (55 Da, Ligand)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Human fdx2-bound core isc complex (nfs1-isd11-acp1-iscu2-fdx2) (Complex from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Structure of the core ISC complex under turnover conditions (frataxin-bound)
Resolution: 2.49 Å
EM Method: Single-particle
Fitted PDBs: 8rme
Q-score: 0.615
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Frataxin mature form (14 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Structure of the core ISC complex under turnover conditions (FDX2-bound in proximal conformation)
Resolution: 2.33 Å
EM Method: Single-particle
Fitted PDBs: 8rmf
Q-score: 0.633
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Fe (ii) ion (55 Da, Ligand)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Structure of the core ISC complex under turnover conditions (FDX2-bound in distal conformation)
Resolution: 2.46 Å
EM Method: Single-particle
Fitted PDBs: 8rmg
Q-score: 0.598
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Fe (ii) ion (55 Da, Ligand)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Resolution: 2.49 Å
EM Method: Single-particle
Fitted PDBs: 8pk8
Q-score: 0.661
Schulz V, Steinhilper R, Oltmanns J, Freibert SA, Krapoth N, Linne U, Welsch S, Hoock MH, Schunemann V, Murphy BJ, Lill R
Nat Commun (2024) 15 pp. 3269-3269 [ Pubmed: 38627381 DOI: doi:10.1038/s41467-024-47310-8 ]
- Iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Fe (ii) ion (55 Da, Ligand)
- Iron-bound human nfs1-isd11-acp1-iscu2-fxn (fe-niaux) complex during persulfide transfer (Complex from Homo sapiens)
- Frataxin mature form (14 kDa, Protein from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- Cysteine desulfurase (44 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Resolution: 2.58 Å
EM Method: Single-particle
Fitted PDBs: 8pk9
Q-score: 0.661
Schulz V, Steinhilper R, Oltmanns J, Freibert SA, Krapoth N, Linne U, Welsch S, Hoock MH, Schunemann V, Murphy BJ, Lill R
Nat Commun (2024) 15 pp. 3269-3269 [ Pubmed: 38627381 DOI: doi:10.1038/s41467-024-47310-8 ]
- Iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Fe (ii) ion (55 Da, Ligand)
- Iron-bound human nfs1-isd11-acp1-iscu2-fxn (fe-niaux) complex during persulfide transfer (Complex from Homo sapiens)
- Frataxin mature form (14 kDa, Protein from Homo sapiens)
- Cysteine desulfurase (44 kDa, Protein from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 8pka
Q-score: 0.624
Schulz V, Steinhilper R, Oltmanns J, Freibert SA, Krapoth N, Linne U, Welsch S, Hoock MH, Schunemann V, Murphy BJ, Lill R
Nat Commun (2024) 15 pp. 3269-3269 [ Pubmed: 38627381 DOI: doi:10.1038/s41467-024-47310-8 ]
- Iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Acyl carrier protein (Complex from Escherichia coli BL21(DE3))
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Fe (ii) ion (55 Da, Ligand)
- Cysteine desulfurase, lyr motif-containing protein 4 and iron-sulfur cluster assembly enzyme iscu (Complex from Homo sapiens)
- Iron-bound human nfs1-isd11-acp1-iscu2-fxn (fe-niaux) complex during persulfide transfer (Complex)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- Cysteine desulfurase (44 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7z0s
Q-score: 0.64
Steinhilper R, Hoff G, Heider J, Murphy BJ
Nat Commun (2022) 13 pp. 5395-5395 [ Pubmed: 36104349 DOI: doi:10.1038/s41467-022-32831-x ]
- Formate hydrogenlyase subunit 4 (33 kDa, Protein from Escherichia coli K-12)
- Formate hydrogenlyase subunit 6 (20 kDa, Protein from Escherichia coli K-12)
- Nickel (ii) ion (58 Da, Ligand)
- Carbonmonoxide-(dicyano) iron (135 Da, Ligand)
- Formate hydrogenlyase subunit 7 (28 kDa, Protein from Escherichia coli K-12)
- Phosphatidylethanolamine (734 Da, Ligand)
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- Formate hydrogenlyase subunit 3 (64 kDa, Protein from Escherichia coli K-12)
- Formate hydrogenlyase subunit 2 (21 kDa, Protein from Escherichia coli K-12)
- 1-cis-9-octadecanoyl-2-cis-9-hexadecanoyl phosphatidyl glycerol (746 Da, Ligand)
- Escherichia coli formate hydrogenlyase complex without formate dehydrogenase h (Complex from Escherichia coli)
- Formate hydrogenlyase subunit 5 (66 kDa, Protein from Escherichia coli K-12)
- Iron/sulfur cluster (351 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- Water (18 Da, Ligand)
- Fe (iii) ion (55 Da, Ligand)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7z0t
Q-score: 0.533
Steinhilper R, Hoff G, Heider J, Murphy BJ
Nat Commun (2022) 13 pp. 5395-5395 [ Pubmed: 36104349 DOI: doi:10.1038/s41467-022-32831-x ]
- Formate hydrogenlyase subunit 4 (33 kDa, Protein from Escherichia coli K-12)
- Formate hydrogenlyase subunit 6 (20 kDa, Protein from Escherichia coli K-12)
- Nickel (ii) ion (58 Da, Ligand)
- Carbonmonoxide-(dicyano) iron (135 Da, Ligand)
- Formate hydrogenlyase subunit 7 (28 kDa, Protein from Escherichia coli K-12)
- Formate dehydrogenase h (79 kDa, Protein from Escherichia coli K-12)
- Formate hydrogenlyase subunit 3 (64 kDa, Protein from Escherichia coli K-12)
- Formate hydrogenlyase subunit 2 (21 kDa, Protein from Escherichia coli K-12)
- 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide (740 Da, Ligand)
- Molybdenum(vi) ion (95 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Formate hydrogenlyase subunit 5 (66 kDa, Protein from Escherichia coli K-12)
- Fe (iii) ion (55 Da, Ligand)
- Escherichia coli formate hydrogenlyase complex (Complex from Escherichia coli)