Structure of the FDX2-bound core ISC complex (proximal conformation)
Resolution: 2.26 Å
EM Method: Single-particle
Fitted PDBs: 8rmc
Q-score: 0.702
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Human fdx2-bound core isc complex (nfs1-isd11-acp1-iscu2-fdx2) (Complex from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Structure of the FDX2-bound core ISC complex (distal conformation)
Resolution: 2.52 Å
EM Method: Single-particle
Fitted PDBs: 8rmd
Q-score: 0.642
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Human fdx2-bound core isc complex (nfs1-isd11-acp1-iscu2-fdx2) (Complex from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Structure of the core ISC complex under turnover conditions (frataxin-bound)
Resolution: 2.49 Å
EM Method: Single-particle
Fitted PDBs: 8rme
Q-score: 0.615
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Frataxin mature form (14 kDa, Protein from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
Structure of the core ISC complex under turnover conditions (FDX2-bound in proximal conformation)
Resolution: 2.33 Å
EM Method: Single-particle
Fitted PDBs: 8rmf
Q-score: 0.633
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
Structure of the core ISC complex under turnover conditions (FDX2-bound in distal conformation)
Resolution: 2.46 Å
EM Method: Single-particle
Fitted PDBs: 8rmg
Q-score: 0.598
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
Resolution: 2.49 Å
EM Method: Single-particle
Fitted PDBs: 8pk8
Q-score: 0.661
Schulz V, Steinhilper R, Oltmanns J, Freibert SA, Krapoth N, Linne U, Welsch S, Hoock MH, Schunemann V, Murphy BJ, Lill R
Nat Commun (2024) 15 pp. 3269-3269 [ DOI: doi:10.1038/s41467-024-47310-8 Pubmed: 38627381 ]
- Water (18 Da, Ligand)
- Cysteine desulfurase (44 kDa, Protein from Homo sapiens)
- Iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Frataxin mature form (14 kDa, Protein from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Iron-bound human nfs1-isd11-acp1-iscu2-fxn (fe-niaux) complex during persulfide transfer (Complex from Homo sapiens)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Resolution: 2.58 Å
EM Method: Single-particle
Fitted PDBs: 8pk9
Q-score: 0.661
Schulz V, Steinhilper R, Oltmanns J, Freibert SA, Krapoth N, Linne U, Welsch S, Hoock MH, Schunemann V, Murphy BJ, Lill R
Nat Commun (2024) 15 pp. 3269-3269 [ DOI: doi:10.1038/s41467-024-47310-8 Pubmed: 38627381 ]
- Water (18 Da, Ligand)
- Iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Cysteine desulfurase (44 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Frataxin mature form (14 kDa, Protein from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Iron-bound human nfs1-isd11-acp1-iscu2-fxn (fe-niaux) complex during persulfide transfer (Complex from Homo sapiens)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 8pka
Q-score: 0.624
Schulz V, Steinhilper R, Oltmanns J, Freibert SA, Krapoth N, Linne U, Welsch S, Hoock MH, Schunemann V, Murphy BJ, Lill R
Nat Commun (2024) 15 pp. 3269-3269 [ DOI: doi:10.1038/s41467-024-47310-8 Pubmed: 38627381 ]
- Water (18 Da, Ligand)
- Cysteine desulfurase, lyr motif-containing protein 4 and iron-sulfur cluster assembly enzyme iscu (Complex from Homo sapiens)
- Iron-bound human nfs1-isd11-acp1-iscu2-fxn (fe-niaux) complex during persulfide transfer (Complex)
- Cysteine desulfurase (44 kDa, Protein from Homo sapiens)
- Acyl carrier protein (Complex from Escherichia coli BL21(DE3))
- Fe (ii) ion (55 Da, Ligand)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
CryoEM structure of Polytomella F-ATP synthase, Full dimer, composite map
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6rd4
Q-score: 0.557
Murphy BJ, Klusch N, Langer J, Mills DJ, Yildiz O, Kuhlbrandt W
Science (2019) 364 [ DOI: doi:10.1126/science.aaw9128 Pubmed: 31221832 ]
- Mitochondrial f1f0 atp synthase associated 32 kda protein (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa6 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Atp synthase associated protein asa1 (68 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase subunit alpha (60 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Magnesium ion (24 Da, Ligand)
- Mitochondrial f-atp synthase dimer from polytomella sp. pringsheim 198.80 (1 MDa, Complex from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase associated protein asa4 (31 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase associated protein asa7 (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial f1f0 atp synthase associated 14 kda protein (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit c (12 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Zinc ion (65 Da, Ligand)
- Mitochondrial atp synthase subunit 6 (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Asa-10: polytomella f-atp synthase associated subunit 10 (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa8 (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa9 (11 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit oscp (25 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Water (18 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Epsilon: polytomella f-atp synthase epsilon subunit (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa2 (44 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase gamma chain, mitochondrial (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit delta (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase subunit beta (61 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Resolution: 2.69 Å
EM Method: Single-particle
Fitted PDBs: 6rd5
Q-score: 0.601
Murphy BJ, Klusch N, Langer J, Mills DJ, Yildiz O, Kuhlbrandt W
Science (2019) 364 [ DOI: doi:10.1126/science.aaw9128 Pubmed: 31221832 ]
- Mitochondrial f1f0 atp synthase associated 32 kda protein (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa9 (11 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial f1f0 atp synthase associated 14 kda protein (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Water (18 Da, Ligand)
- Mitochondrial atp synthase subunit asa6 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Zinc ion (65 Da, Ligand)
- Atp synthase associated protein asa1 (68 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit 6 (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Asa-10: polytomella f-atp synthase associated subunit 10 (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- (1s)-2-{[(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (720 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Mitochondrial atp synthase subunit asa8 (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Polytomella f-atp synthase, focussed refinement of fo and peripheral stalk, c2 symmetry (Complex from Polytomella sp. Pringsheim 198.80)