E. coli 50S ribosomal subunit in complex with PrAMP rumicidin-2 (focused refinement)
Resolution: 1.95 Å
EM Method: Single-particle
Fitted PDBs: 9d89
Q-score: 0.692
Panteleev PV, Pichkur EB, Kruglikov RN, Paleskava A, Shulenina OV, Bolosov IA, Bogdanov IV, Safronova VN, Balandin SV, Marina VI, Kombarova TI, Korobova OV, Shamova OV, Myasnikov AG, Borzilov AI, Osterman IA, Sergiev PV, Bogdanov AA, Dontsova OA, Konevega AL, Ovchinnikova TV
Nat Commun (2024) 15 pp. 8925-8925 [ Pubmed: 39414793 DOI: doi:10.1038/s41467-024-53309-y ]
- (2s)-2-amino-4-(methylsulfanyl)butane-1-thiol (151 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul29 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul6 (18 kDa, Protein from Escherichia coli)
- Potassium ion (39 Da, Ligand)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
- Water (18 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
- 23s rrna (885 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl25 (10 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul23 (10 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul24 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl20 (13 kDa, Protein from Escherichia coli)
- 5s rrna (38 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul13 (16 kDa, Protein from Escherichia coli)
- Rumicidin-2 (12-27) (2 kDa, Protein from Beatragus hunteri)
- 50s ribosomal protein l9 (4 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
- E. coli 70s ribosome in complex with pramp rumicidin-2 (Complex from Escherichia coli)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
Cryo-EM structure of mouse heavy-chain apoferritin
Omadacycline and spectinomycin bound to the 30S ribosomal subunit head
Resolution: 2.06 Å
EM Method: Single-particle
Fitted PDBs: 8ca7
Q-score: 0.666
Paternoga H, Crowe-McAuliffe C, Bock LV, Koller TO, Morici M, Beckert B, Myasnikov AG, Grubmuller H, Novacek J, Wilson DN
Nat Struct Mol Biol (2023) 30 pp. 1380-1392 [ Pubmed: 37550453 DOI: doi:10.1038/s41594-023-01047-y ]
- Potassium ion (39 Da, Ligand)
- Small ribosomal subunit protein us9 (14 kDa, Protein from Escherichia coli BW25113)
- Magnesium ion (24 Da, Ligand)
- Small ribosomal subunit protein us13 (13 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli BW25113)
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli BW25113)
- Omadacycline (556 Da, Ligand)
- Small ribosomal subunit protein us5 (17 kDa, Protein from Escherichia coli BW25113)
- Spectinomycin (332 Da, Ligand)
- Small ribosomal subunit protein us19 (10 kDa, Protein from Escherichia coli BW25113)
- Ribosomal small subunit head (Complex from Escherichia coli BW25113)
- Water (18 Da, Ligand)
- 16s rrna (499 kDa, RNA from Escherichia coli BW25113)
- Small ribosomal subunit protein us14 (11 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us10 (11 kDa, Protein from Escherichia coli BW25113)
Eravacycline bound to the 30S head
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 8cf8
Q-score: 0.687
Paternoga H, Crowe-McAuliffe C, Bock LV, Koller TO, Morici M, Beckert B, Myasnikov AG, Grubmuller H, Novacek J, Wilson DN
Nat Struct Mol Biol (2023) 30 pp. 1380-1392 [ DOI: doi:10.1038/s41594-023-01047-y Pubmed: 37550453 ]
- Magnesium ion (24 Da, Ligand)
- 16s rrna (499 kDa, RNA from Escherichia coli BW25113)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli BW25113)
- Potassium ion (39 Da, Ligand)
- Small ribosomal subunit protein us9 (14 kDa, Protein from Escherichia coli BW25113)
- Water (18 Da, Ligand)
- Small ribosomal subunit protein us13 (13 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us19 (10 kDa, Protein from Escherichia coli BW25113)
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us14 (11 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us10 (11 kDa, Protein from Escherichia coli BW25113)
- Eravacycline (558 Da, Ligand)
- 70s ribosomes with antibiotic cocktail (Complex from Escherichia coli BW25113)
Tiamulin bound to the 50S subunit
Resolution: 1.66 Å
EM Method: Single-particle
Fitted PDBs: 8cgv
Q-score: 0.807
Paternoga H, Crowe-McAuliffe C, Bock LV, Koller TO, Morici M, Beckert B, Myasnikov AG, Grubmuller H, Novacek J, Wilson DN
Nat Struct Mol Biol (2023) 30 pp. 1380-1392 [ Pubmed: 37550453 DOI: doi:10.1038/s41594-023-01047-y ]
- Large ribosomal subunit protein ul24 (11 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul14 (13 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul4 (22 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl32 (6 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl27 (9 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul22 (12 kDa, Protein from Escherichia coli BW25113)
- Tiamulin (493 Da, Ligand)
- Large ribosomal subunit protein bl33 (6 kDa, Protein from Escherichia coli BW25113)
- Potassium ion (39 Da, Ligand)
- Large ribosomal subunit protein bl36a (4 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl20 (13 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl9 (15 kDa, Protein from Escherichia coli BW25113)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli BW25113)
- Water (18 Da, Ligand)
- Pentacycline (551 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 23s rrna (941 kDa, RNA from Escherichia coli BW25113)
- Large ribosomal subunit protein ul3 (22 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul13 (16 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul30 (6 kDa, Protein from Escherichia coli BW25113)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul18 (12 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul16 (15 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul6 (18 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl21 (11 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl35 (7 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul29 (7 kDa, Protein from Escherichia coli BW25113)
- 70s ribosomes with antibiotic cocktail (Complex from Escherichia coli BW25113)
- Large ribosomal subunit protein bl19 (13 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl28 (9 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul2 (29 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl34 (5 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul23 (11 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl17 (14 kDa, Protein from Escherichia coli BW25113)
- 5s rrna (38 kDa, RNA from Escherichia coli BW25113)
Streptomycin and Hygromycin B bound to the 30S body
Resolution: 2.08 Å
EM Method: Single-particle
Fitted PDBs: 8cai
Q-score: 0.698
Paternoga H, Crowe-McAuliffe C, Bock LV, Koller TO, Morici M, Beckert B, Myasnikov AG, Grubmuller H, Novacek J, Wilson DN
Nat Struct Mol Biol (2023) 30 pp. 1380-1392 [ DOI: doi:10.1038/s41594-023-01047-y Pubmed: 37550453 ]
- Small ribosomal subunit protein us8 (14 kDa, Protein from Escherichia coli BW25113)
- Hygromycin b variant (527 Da, Ligand)
- Small ribosomal subunit protein bs21 (8 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us15 (10 kDa, Protein from Escherichia coli BW25113)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli BW25113)
- Potassium ion (39 Da, Ligand)
- Water (18 Da, Ligand)
- Small ribosomal subunit protein us5 (17 kDa, Protein from Escherichia coli BW25113)
- 16s rrna (499 kDa, RNA from Escherichia coli BW25113)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein bs18 (9 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us17 (9 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein bs6, non-modified isoform (15 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us11 (13 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us12 (13 kDa, Protein from Escherichia coli BW25113)
- [(2~{s},3~{s},4~{s},5~{r},6~{s})-2-[(2~{r},3~{r},4~{r},5~{s})-2-[(1~{r},2~{s},3~{r},4~{r},5~{s},6~{r})-2,4-bis[[azaniumylidene(azanyl)methyl]amino]-3,5,6-tris(oxidanyl)cyclohexyl]oxy-4-[bis(oxidanyl)methyl]-5-methyl-4-oxidanyl-oxolan-3-yl]oxy-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-3-yl]-methyl-azanium (602 Da, Ligand)
- Spectinomycin (332 Da, Ligand)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli BW25113)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli BW25113)
- 70s ribosomes with antibiotic cocktail (Complex from Escherichia coli BW25113)
- Magnesium ion (24 Da, Ligand)
Evernimicin bound to the 50S subunit
Resolution: 1.86 Å
EM Method: Single-particle
Fitted PDBs: 8cam
Q-score: 0.731
Paternoga H, Crowe-McAuliffe C, Bock LV, Koller TO, Morici M, Beckert B, Myasnikov AG, Grubmuller H, Novacek J, Wilson DN
Nat Struct Mol Biol (2023) 30 pp. 1380-1392 [ DOI: doi:10.1038/s41594-023-01047-y Pubmed: 37550453 ]
- Large ribosomal subunit protein bl17 (14 kDa, Protein from Escherichia coli BW25113)
- Potassium ion (39 Da, Ligand)
- Large ribosomal subunit protein bl33 (6 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul23 (11 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl35 (7 kDa, Protein from Escherichia coli BW25113)
- Water (18 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Evernimicin (1 kDa, Ligand)
- Acetate ion (59 Da, Ligand)
- Large ribosomal subunit protein bl20 (13 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul13 (16 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul2 (29 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul4 (22 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl28 (9 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul3 (22 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl19 (13 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl9 (15 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul29 (7 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul30 (6 kDa, Protein from Escherichia coli BW25113)
- 5s rrna (38 kDa, RNA from Escherichia coli BW25113)
- Large ribosomal subunit protein bl32 (6 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul24 (11 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul22 (12 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul6 (18 kDa, Protein from Escherichia coli BW25113)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl27 (9 kDa, Protein from Escherichia coli BW25113)
- 70s ribosomes with antibiotic cocktail (Complex from Escherichia coli BW25113)
- 23s rrna (941 kDa, RNA from Escherichia coli BW25113)
- Large ribosomal subunit protein bl34 (5 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl36a (4 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul14 (13 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul16 (15 kDa, Protein from Escherichia coli BW25113)
- Magnesium ion (24 Da, Ligand)
- Large ribosomal subunit protein bl21 (11 kDa, Protein from Escherichia coli BW25113)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli BW25113)
Resolution: 2.11 Å
EM Method: Single-particle
Fitted PDBs: 8caz
Q-score: 0.701
Paternoga H, Crowe-McAuliffe C, Bock LV, Koller TO, Morici M, Beckert B, Myasnikov AG, Grubmuller H, Novacek J, Wilson DN
Nat Struct Mol Biol (2023) 30 pp. 1380-1392 [ DOI: doi:10.1038/s41594-023-01047-y Pubmed: 37550453 ]
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us9 (14 kDa, Protein from Escherichia coli BW25113)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us19 (10 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us14 (11 kDa, Protein from Escherichia coli BW25113)
- 70s ribosomes with antibiotic cocktail (Complex from Escherichia coli BW25113)
- Small ribosomal subunit protein us13 (13 kDa, Protein from Escherichia coli BW25113)
- 16s rrna (499 kDa, RNA from Escherichia coli BW25113)
- Potassium ion (39 Da, Ligand)
- Large ribosomal subunit protein bl31a (7 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us10 (11 kDa, Protein from Escherichia coli BW25113)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli BW25113)
Retapamulin and Capreomycin bound to the 50S subunit
Resolution: 1.83 Å
EM Method: Single-particle
Fitted PDBs: 8ceu
Q-score: 0.756
Paternoga H, Crowe-McAuliffe C, Bock LV, Koller TO, Morici M, Beckert B, Myasnikov AG, Grubmuller H, Novacek J, Wilson DN
Nat Struct Mol Biol (2023) 30 pp. 1380-1392 [ Pubmed: 37550453 DOI: doi:10.1038/s41594-023-01047-y ]
- Large ribosomal subunit protein ul24 (11 kDa, Protein from Escherichia coli BW25113)
- Capreomycin ia (686 Da, Protein from Streptomyces californicus)
- Large ribosomal subunit protein ul14 (13 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul4 (22 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl32 (6 kDa, Protein from Escherichia coli BW25113)
- Retapamulin (517 Da, Ligand)
- Large ribosomal subunit protein bl27 (9 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul22 (12 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl33 (6 kDa, Protein from Escherichia coli BW25113)
- Potassium ion (39 Da, Ligand)
- Large ribosomal subunit protein bl36a (4 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl20 (13 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl9 (15 kDa, Protein from Escherichia coli BW25113)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli BW25113)
- Water (18 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 23s rrna (941 kDa, RNA from Escherichia coli BW25113)
- Large ribosomal subunit protein ul3 (22 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul13 (16 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul30 (6 kDa, Protein from Escherichia coli BW25113)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul18 (12 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul16 (15 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul6 (18 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul5 (20 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl21 (11 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl35 (7 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul29 (7 kDa, Protein from Escherichia coli BW25113)
- 70s ribosomes with antibiotic cocktail (Complex from Escherichia coli BW25113)
- Large ribosomal subunit protein bl19 (13 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl28 (9 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul2 (29 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl34 (5 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul23 (11 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl17 (14 kDa, Protein from Escherichia coli BW25113)
- 5s rrna (38 kDa, RNA from Escherichia coli BW25113)
Tetracycline bound to the 30S head
Resolution: 1.82 Å
EM Method: Single-particle
Fitted PDBs: 8cf1
Q-score: 0.743
Paternoga H, Crowe-McAuliffe C, Bock LV, Koller TO, Morici M, Beckert B, Myasnikov AG, Grubmuller H, Novacek J, Wilson DN
Nat Struct Mol Biol (2023) 30 pp. 1380-1392 [ Pubmed: 37550453 DOI: doi:10.1038/s41594-023-01047-y ]
- Potassium ion (39 Da, Ligand)
- Small ribosomal subunit protein us9 (14 kDa, Protein from Escherichia coli BW25113)
- Magnesium ion (24 Da, Ligand)
- 70s ribosomes with antibiotic cocktail (Complex from Escherichia coli BW25113)
- Small ribosomal subunit protein us13 (13 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli BW25113)
- 16s rrna (499 kDa, RNA from Escherichia coli BW25113)
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us5 (17 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us19 (10 kDa, Protein from Escherichia coli BW25113)
- Water (18 Da, Ligand)
- Tetracycline (444 Da, Ligand)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us14 (11 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us10 (11 kDa, Protein from Escherichia coli BW25113)