CryoEM structure of E.coli RNA polymerase paused elongation complex bound to NusA
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6flq
Q-score: -0.011
Guo X, Myasnikov AG, Chen J, Crucifix C, Papai G, Takacs M, Schultz P, Weixlbaumer A
Mol Cell (2018) 69 pp. 816-827.e4 [ Pubmed: 29499136 DOI: doi:10.1016/j.molcel.2018.02.008 ]
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
- Dna (39-mer) (11 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Rna (5'-r(*cp*cp*up*gp*ap*up*cp*ap*gp*gp*cp*gp*ap*up*gp*up*gp*up*gp*cp*u)-3') (6 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
- Rna polymerase, nusa (Complex from Escherichia coli K-12)
- Nucleic acids (Complex)
- Transcription termination/antitermination protein nusa (54 kDa, Protein from Escherichia coli (strain K12))
- Cryo-em structure of e. coli rna polymerase paused elongation complex bound to nusa (Complex)
- Dna (31-mer) (9 kDa, DNA from synthetic construct)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6flp
Q-score: 0.334
Guo X, Myasnikov AG, Chen J, Crucifix C, Papai G, Takacs M, Schultz P, Weixlbaumer A
Mol Cell (2018) 69 pp. 816-827.e4 [ Pubmed: 29499136 DOI: doi:10.1016/j.molcel.2018.02.008 ]
- Rna polymerase and nusa (Complex from Escherichia coli K-12)
- Dna (39-mer) (11 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Nucleic acids (Complex from synthetic construct)
- Cryo-em structure of e. coli rna polymerase paused elongation complex without rna hairpin bound to nusa (Complex)
- Rna (5'-r(p*gp*ap*up*gp*up*gp*up*gp*cp*u)-3') (3 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli (strain K12))
- Magnesium ion (24 Da, Ligand)
- Dna (30-mer) (9 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
Cryo-EM structure of the yeast chromatin modifying complex SAGA
Sharov G, Voltz K, Durand A, Kolesnikova O, Papai G, Myasnikov AG, Dejaegere A, Shem AB, Schultz P
Nat Commun (2017) 8 pp. 1556-1556 [ DOI: doi:10.1038/s41467-017-01564-7 Pubmed: 29146944 ]
Structure of Tra1 subunit within the chromatin modifying complex SAGA
Resolution: 5.7 Å
EM Method: Single-particle
Fitted PDBs: 5oej
Q-score: 0.219
Sharov G, Voltz K, Durand A, Kolesnikova O, Papai G, Myasnikov AG, Dejaegere A, Shem AB, Schultz P
Nat Commun (2017) 8 pp. 1556-1556 [ DOI: doi:10.1038/s41467-017-01564-7 Pubmed: 29146944 ]
- Tra1 subunit within the chromatin modifying complex saga (438 kDa, Protein from Komagataella pastoris)
- Tra1 subunit of saga complex (400 kDa, Complex from Komagataella pastoris)