Cryo-EM structure of the gastric proton pump
Abe K, Ozako M, Inukai M, Matsuyuki Y, Kitayama S, Kanai C, Nagai C, Gopalasingam CC, Gerle C, Shigematsu H, Umekubo N, Yokoshima S, Yoshimori A
Communications Biology (2023) 6 [ Pubmed: 37726448 DOI: 10.1038/s42003-023-05334-8 ]
Image sets:
- K3 movies for gastric proton pump with DQ-18
- K3 movies for gastric proton pump with DQ-21
- K3 movies for gastric proton pump with DQ-02
- K3 movies for gastric proton pump with DQ-06
Cryo-EM structure of the gastric proton pump with bound DQ-02
Resolution: 2.1 Å
EM Method: Single-particle
Fitted PDBs: 8ijv
Q-score: 0.687
Abe K, Ozako M, Inukai M, Matsuyuki Y, Kitayama S, Kanai C, Nagai C, Gopalasingam CC, Gerle C, Shigematsu H, Umekubo N, Yokoshima S, Yoshimori A
Commun Biol (2023) 6 pp. 956-956 [ Pubmed: 37726448 DOI: doi:10.1038/s42003-023-05334-8 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- 4-[[5-chloranyl-2-(4-chlorophenyl)phenyl]methoxy]-n-methyl-but-2-yn-1-amine (334 Da, Ligand)
- Sodium/potassium-transporting atpase subunit alpha (114 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Potassium-transporting atpase subunit beta (33 kDa, Protein from Sus scrofa)
- Cholesterol (386 Da, Ligand)
- Water (18 Da, Ligand)
- Hetero dimer of alpha and beta subunit (135 kDa, Complex from Sus scrofa)
Cryo-EM structure of the gastric proton pump with bound DQ-06
Resolution: 2.19 Å
EM Method: Single-particle
Fitted PDBs: 8ijw
Q-score: 0.658
Abe K, Ozako M, Inukai M, Matsuyuki Y, Kitayama S, Kanai C, Nagai C, Gopalasingam CC, Gerle C, Shigematsu H, Umekubo N, Yokoshima S, Yoshimori A
Commun Biol (2023) 6 pp. 956-956 [ Pubmed: 37726448 DOI: doi:10.1038/s42003-023-05334-8 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- N-methyl-1-[4-[(2-phenylmethoxycyclohexa-1,3-dien-1-yl)methoxy]cyclohexa-1,3-dien-1-yl]methanamine (337 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Sodium/potassium-transporting atpase subunit alpha (114 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Potassium-transporting atpase subunit beta (33 kDa, Protein from Sus scrofa)
- Cholesterol (386 Da, Ligand)
- Water (18 Da, Ligand)
- Hetero dimer of alpha and beta subunit (135 kDa, Complex from Sus scrofa)
Cryo-EM structure of the gastric proton pump with bound DQ-18
Resolution: 2.08 Å
EM Method: Single-particle
Fitted PDBs: 8ijx
Q-score: 0.68
Abe K, Ozako M, Inukai M, Matsuyuki Y, Kitayama S, Kanai C, Nagai C, Gopalasingam CC, Gerle C, Shigematsu H, Umekubo N, Yokoshima S, Yoshimori A
Commun Biol (2023) 6 pp. 956-956 [ Pubmed: 37726448 DOI: doi:10.1038/s42003-023-05334-8 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 1-[4-[(5-chloranyl-2-phenylmethoxy-phenyl)methoxy]phenyl]-n-methyl-methanamine (367 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Sodium/potassium-transporting atpase subunit alpha (114 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Potassium-transporting atpase subunit beta (33 kDa, Protein from Sus scrofa)
- Cholesterol (386 Da, Ligand)
- Water (18 Da, Ligand)
- Hetero dimer of alpha and beta subunit (135 kDa, Complex from Sus scrofa)
Cryo-EM structure of the gastric proton pump with bound DQ-21
Resolution: 2.26 Å
EM Method: Single-particle
Fitted PDBs: 8jmn
Q-score: 0.67
Abe K, Ozako M, Inukai M, Matsuyuki Y, Kitayama S, Kanai C, Nagai C, Gopalasingam CC, Gerle C, Shigematsu H, Umekubo N, Yokoshima S, Yoshimori A
Commun Biol (2023) 6 pp. 956-956 [ Pubmed: 37726448 DOI: doi:10.1038/s42003-023-05334-8 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Sodium/potassium-transporting atpase subunit alpha (114 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Potassium-transporting atpase subunit beta (33 kDa, Protein from Sus scrofa)
- Cholesterol (386 Da, Ligand)
- 1-[4-[[2-[(4-chlorophenyl)methoxy]phenyl]methoxy]phenyl]-n-methyl-methanamine (367 Da, Ligand)
- Water (18 Da, Ligand)
- Hetero dimer of alpha and beta subunit (135 kDa, Complex from Sus scrofa)