Transcription antitermination complex: "pre-engaged" Qlambda-loading complex
Resolution: 3.13 Å
EM Method: Single-particle
Fitted PDBs: 7ubm
Q-score: 0.461
Yin Z, Bird JG, Kaelber JT, Nickels BE, Ebright RH
PNAS (2022) 119 pp. e2205278119-e2205278119 [ DOI: doi:10.1073/pnas.2205278119 Pubmed: 35951650 ]
- Dna (52-mer) (18 kDa, DNA from Escherichia coli)
- Rna polymerase sigma factor rpod (71 kDa, Protein from Escherichia coli)
- Dna (53-mer) (18 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Rna (5'-r(*up*gp*gp*gp*ap*gp*ap*gp*gp*up*a)-3') (3 kDa, RNA from Escherichia coli)
- Transcription antitermination complex: "pre-engaged" qlambda-loading complex (500 kDa, Complex from Escherichia coli)
- Antitermination protein q (22 kDa, Protein from Escherichia phage Lambda)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (158 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
Transcription antitermination complex: NusA-containing "engaged" Qlambda-loading complex
Resolution: 3.36 Å
EM Method: Single-particle
Fitted PDBs: 7ubn
Q-score: 0.435
Yin Z, Bird JG, Kaelber JT, Nickels BE, Ebright RH
PNAS (2022) 119 pp. e2205278119-e2205278119 [ DOI: doi:10.1073/pnas.2205278119 Pubmed: 35951650 ]
- Transcription termination/antitermination protein nusa (57 kDa, Protein from Escherichia coli)
- Rna polymerase sigma factor rpod (71 kDa, Protein from Escherichia coli)
- Dna (52-mer) (18 kDa, DNA from Escherichia phage Lambda)
- Dna (53-mer) (18 kDa, DNA from Escherichia phage Lambda)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Rna (5'-r(*up*gp*gp*gp*ap*gp*ap*gp*gp*up*a)-3') (3 kDa, RNA from Escherichia coli)
- Antitermination protein (22 kDa, Protein from Escherichia coli)
- Transcription antitermination complex: nusa-containing "engaged" qlambda-loading complex (550 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (158 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
Escherichia coli sigma 70-dependent paused transcription elongation complex
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7n4e
Q-score: 0.343
Pukhrambam C, Molodtsov V, Kooshkbaghi M, Tareen A, Vu H, Skalenko KS, Su M, Yin Z, Winkelman JT, Kinney JB, Ebright RH, Nickels BE
PNAS (2022) 119 pp. e2201301119-e2201301119 [ DOI: doi:10.1073/pnas.2201301119 Pubmed: 35653571 ]
- Dna (61-mer) (18 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna (61-mer) (18 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- 5'-r(*up*up*cp*gp*gp*ap*gp*ap*gp*gp*up*a)-3' (3 kDa, RNA from synthetic construct)
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Escherichia coli sigma 70-dependent paused transcription elongation complex (Complex from Escherichia coli)
Cryo-EM structure of Thermus thermophilus reiterative transcription complex with 11nt oligo-G RNA
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7rdq
Q-score: 0.516
Liu Y, Yu L, Pukhrambam C, Winkelman JT, Firlar E, Kaelber JT, Zhang Y, Nickels BE, Ebright RH
PNAS (2022) 119 [ Pubmed: 35082149 DOI: doi:10.1073/pnas.2115746119 ]
- Reiterative transcription initiation complex with 11nt oligo-g rna (425 kDa, Complex from Thermus thermophilus HB8)
- Dna-directed rna polymerase subunit beta (125 kDa, Protein from Thermus thermophilus HB8)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Thermus thermophilus HB8)
- Dna (31-mer) nontemplate strand (10 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta' (170 kDa, Protein from Thermus thermophilus HB8)
- Rna polymerase sigma factor siga (48 kDa, Protein from Thermus thermophilus HB8)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Thermus thermophilus HB8)
- Dna (31-mer) template strand (10 kDa, DNA from synthetic construct)
- Rna (5'-r(p*gp*gp*gp*gp*gp*gp*gp*gp*gp*gp*g)-3') (3 kDa, RNA from synthetic construct)