Resolution: 3.36 Å
EM Method: Single-particle
Fitted PDBs: 8thi
Q-score: 0.411
Currie MJ, Davies JS, Scalise M, Gulati A, Wright JD, Newton-Vesty MC, Abeysekera GS, Subramanian R, Wahlgren WY, Friemann R, Allison JR, Mace PD, Griffin MDW, Demeler B, Wakatsuki S, Drew D, Indiveri C, Dobson RCJ, North RA
eLife (2024) 12 [ DOI: doi:10.7554/eLife.92307 Pubmed: 38349818 ]
- Sialic acid trap transporter permease protein siat (72 kDa, Protein from Haemophilus influenzae Rd KW20)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Hisiaqm transporter protein solubilised in amphipol a8-35 (Complex from Haemophilus influenzae Rd KW20)
- Sodium ion (22 Da, Ligand)
Resolution: 2.99 Å
EM Method: Single-particle
Fitted PDBs: 8thj
Q-score: 0.52
Currie MJ, Davies JS, Scalise M, Gulati A, Wright JD, Newton-Vesty MC, Abeysekera GS, Subramanian R, Wahlgren WY, Friemann R, Allison JR, Mace PD, Griffin MDW, Demeler B, Wakatsuki S, Drew D, Indiveri C, Dobson RCJ, North RA
eLife (2024) 12 [ DOI: doi:10.7554/eLife.92307 Pubmed: 38349818 ]
- Sialic acid trap transporter permease protein siat (72 kDa, Protein from Haemophilus influenzae Rd KW20)
- Phosphatidylethanolamine (734 Da, Ligand)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Hisiaqm transporter protein solubilised in amphipol a8-35 (Complex from Haemophilus influenzae Rd KW20)
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 8b01
Q-score: 0.539
Davies JS, Currie MJ, North RA, Scalise M, Wright JD, Copping JM, Remus DM, Gulati A, Morado DR, Jamieson SA, Newton-Vesty MC, Abeysekera GS, Ramaswamy S, Friemann R, Wakatsuki S, Allison JR, Indiveri C, Drew D, Mace PD, Dobson RCJ
Nat Commun (2023) 14 pp. 1120-1120 [ Pubmed: 36849793 DOI: doi:10.1038/s41467-023-36590-1 ]
- Megabody c7hopq (54 kDa, Protein from synthetic construct)
- Docosane (310 Da, Ligand)
- Hexane (86 Da, Ligand)
- Putative trap-type c4-dicarboxylate transport system, large permease component (45 kDa, Protein from Photobacterium profundum SS9)
- Phosphatidylethanolamine (734 Da, Ligand)
- Putative trap-type c4-dicarboxylate transport system, small permease component (19 kDa, Protein from Photobacterium profundum SS9)
- Sodium ion (22 Da, Ligand)
- Tridecane (184 Da, Ligand)
- Decane (142 Da, Ligand)
- Siaqm (Complex from Photobacterium profundum)
- N-octane (114 Da, Ligand)
- Siaqm with megabody (Complex)
- Megabody (Complex from Helicobacter pylori)
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 7qha
Q-score: 0.48
Davies JS, Currie MJ, North RA, Scalise M, Wright JD, Copping JM, Remus DM, Gulati A, Morado DR, Jamieson SA, Newton-Vesty MC, Abeysekera GS, Ramaswamy S, Friemann R, Wakatsuki S, Allison JR, Indiveri C, Drew D, Mace PD, Dobson RCJ
Nat Commun (2023) 14 pp. 1120-1120 [ Pubmed: 36849793 DOI: doi:10.1038/s41467-023-36590-1 ]
- Megabody c7hopq (54 kDa, Protein from synthetic construct)
- Putative trap-type c4-dicarboxylate transport system, large permease component (45 kDa, Protein from Photobacterium profundum SS9)
- Megabody (Complex from Helicobacter pylori)
- Putative trap-type c4-dicarboxylate transport system, small permease component (19 kDa, Protein from Photobacterium profundum SS9)
- Sodium ion (22 Da, Ligand)
- Siaqm (Complex from Photobacterium profundum)
- Siaqm with megabody (Complex)
CryoEM single particle dataset for NanR dimer-DNA hetero-complex.
Venugopal H, Horne CR, Panjikar S, Wood DM, Henrickson A, Brookes E, North RA, Murphy JM, Friemann R, Griffin MDW, Ramm G, Demeler B, Dobson RCJ
Nat Commun (2021) 12 [ Pubmed: 33790291 DOI: 10.1038/s41467-021-22253-6 ]
Image sets:
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6wfq
Q-score: 0.256
Horne CR, Venugopal H, Panjikar S, Wood DM, Henrickson A, Brookes E, North RA, Murphy JM, Friemann R, Griffin MDW, Ramm G, Demeler B, Dobson RCJ
Nat Commun (2021) 12 pp. 1988-1988 [ Pubmed: 33790291 DOI: doi:10.1038/s41467-021-22253-6 ]
- Nanr-dna hetero-complex (72 kDa, Complex from Escherichia coli)
- Hth-type transcriptional repressor nanr (29 kDa, Protein from Escherichia coli)
- Dna (5'-d(p*tp*ap*tp*ap*cp*cp*tp*gp*tp*tp*ap*tp*ap*cp*c)-3') (4 kDa, DNA from synthetic construct)
- Dna (5'-d(p*gp*gp*tp*ap*tp*ap*ap*cp*ap*gp*gp*tp*ap*tp*a)-3') (4 kDa, DNA from synthetic construct)
Hexameric NanR-DNA hetero-complex
Resolution: 8.3 Å
EM Method: Single-particle
Fitted PDBs: 6wg7
Q-score: 0.062
Horne CR, Venugopal H, Panjikar S, Wood DM, Henrickson A, Brookes E, North RA, Murphy JM, Friemann R, Griffin MDW, Ramm G, Demeler B, Dobson RCJ
Nat Commun (2021) 12 pp. 1988-1988 [ Pubmed: 33790291 DOI: doi:10.1038/s41467-021-22253-6 ]
- Dna (35-mer) (10 kDa, DNA from synthetic construct)
- Dna (35-mer) (10 kDa, DNA from synthetic construct)
- Hth-type transcriptional repressor nanr (29 kDa, Protein from Escherichia coli)
- Hexameric nanr-dna hetero-complex (198 kDa, Complex from Escherichia coli)