Human NLRP1 complexed with thioredoxin (focused map)
Zhang Z, Shibata T, Fujimura A, Kitaura J, Miyake K, Ohto U, Shimizu T
Nature (2023) 622 pp. 188-194 [ Pubmed: 37704723 DOI: doi:10.1038/s41586-023-06532-4 ]
- Sftrx (Complex)
- Hnlrp1 (Complex from Spodoptera frugiperda)
- Hnlrp1 complexed with sftrx (Complex from Homo sapiens)
Human NLRP1 complexed with thioredoxin
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7wge
Q-score: 0.384
Zhang Z, Shibata T, Fujimura A, Kitaura J, Miyake K, Ohto U, Shimizu T
Nature (2023) 622 pp. 188-194 [ Pubmed: 37704723 DOI: doi:10.1038/s41586-023-06532-4 ]
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Sftrx (Complex)
- Hnlrp1 complexed with sftrx (Complex from Homo sapiens)
- Nacht, lrr and pyd domains-containing protein 1, n-terminus (101 kDa, Protein from Homo sapiens)
- Hnlrp1 (Complex from Spodoptera frugiperda)
- Magnesium ion (24 Da, Ligand)
- Thioredoxin (11 kDa, Protein from Spodoptera frugiperda)
Structure of bile acid transporter NTCP
Asami J, Kimura KT, Fujita-Fujiharu Y, Ishida H, Zhang Z, Nomura Y, Liu K, Uemura T, Sato Y, Ono M, Yamamoto M, Noda T, Shigematsu H, Drew D, Iwata S, Shimizu T, Nomura N, Ohto U
Nature (2022) [ DOI: 10.1038/s41586-022-04845-4 Pubmed: 35580629 ]
Image sets:
- Raw movies for Human NTCP (Q261A) - YN69202Fab (EMD-31837, PDB 7VAD)
- Raw movies for Rat NTCP (Q261A) - YN69202Fab (EMD-31839, PDB 7VAE)
- Raw movies for Human NTCP (Q261A) - YN69202Fab-myr-preS1 (EMD-31840, PDB 7VAF)
- Raw movies for Bovine NTCP (Q261A) - YN69202Fab (EMD-31838, PDB 7VAE)
- Raw movies for Human NTCP (wildtype) - YN69202Fab (EMD-32759, PDB 7WSI)
Cryo-EM structure of human NTCP complexed with YN69202Fab
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 7vad
Q-score: 0.399
Asami J, Kimura KT, Fujita-Fujiharu Y, Ishida H, Zhang Z, Nomura Y, Liu K, Uemura T, Sato Y, Ono M, Yamamoto M, Noda T, Shigematsu H, Drew D, Iwata S, Shimizu T, Nomura N, Ohto U
Nature (2022) 606 pp. 1021-1026 [ DOI: doi:10.1038/s41586-022-04845-4 Pubmed: 35580629 ]
- Fab light chain from antibody igg clone number yn69202 (24 kDa, Protein from Mus musculus)
- Sodium/bile acid cotransporter (38 kDa, Protein from Homo sapiens)
- Fab (Complex)
- Complex of ntcp and fab (Complex from Homo sapiens)
- Fab heavy chain from antibody igg clone number yn69202 (26 kDa, Protein from Mus musculus)
- Ntcp: (Complex from Mus musculus)
Cryo-EM structure of bovine NTCP complexed with YN69202Fab
Resolution: 3.55 Å
EM Method: Single-particle
Fitted PDBs: 7vae
Q-score: 0.4
Asami J, Kimura KT, Fujita-Fujiharu Y, Ishida H, Zhang Z, Nomura Y, Liu K, Uemura T, Sato Y, Ono M, Yamamoto M, Noda T, Shigematsu H, Drew D, Iwata S, Shimizu T, Nomura N, Ohto U
Nature (2022) 606 pp. 1021-1026 [ DOI: doi:10.1038/s41586-022-04845-4 Pubmed: 35580629 ]
- Ntcp (Complex from Mus musculus)
- Fab light chain from antibody igg clone number yn69202 (24 kDa, Protein from Mus musculus)
- Fab heavy chain from antibody igg clone number yn69202 (26 kDa, Protein from Mus musculus)
- Solute carrier family 10 (sodium/bile acid cotransporter family), member 1 (38 kDa, Protein from Bos taurus)
- Fab (Complex)
- Complex of ntcp and fab (Complex from Homo sapiens)
Cryo-EM structure of Rat NTCP complexed with YN69202Fab
Resolution: 3.11 Å
EM Method: Single-particle
Fitted PDBs: 7vaf
Q-score: 0.461
Asami J, Kimura KT, Fujita-Fujiharu Y, Ishida H, Zhang Z, Nomura Y, Liu K, Uemura T, Sato Y, Ono M, Yamamoto M, Noda T, Shigematsu H, Drew D, Iwata S, Shimizu T, Nomura N, Ohto U
Nature (2022) 606 pp. 1021-1026 [ DOI: doi:10.1038/s41586-022-04845-4 Pubmed: 35580629 ]
- Complex of ntcp and fab (Complex from Mus musculus)
- Ntcp (Complex)
- Sodium/bile acid cotransporter (38 kDa, Protein from Rattus norvegicus)
- Fab (Complex from Rattus norvegicus)
- Fab heavy chain from antibody igg clone number yn69202 (26 kDa, Protein from Mus musculus)
- Fab light chain from antibody igg clone number yn69202 (24 kDa, Protein from Mus musculus)
Resolution: 3.32 Å
EM Method: Single-particle
Fitted PDBs: 7vag
Q-score: 0.401
Asami J, Kimura KT, Fujita-Fujiharu Y, Ishida H, Zhang Z, Nomura Y, Liu K, Uemura T, Sato Y, Ono M, Yamamoto M, Noda T, Shigematsu H, Drew D, Iwata S, Shimizu T, Nomura N, Ohto U
Nature (2022) 606 pp. 1021-1026 [ DOI: doi:10.1038/s41586-022-04845-4 Pubmed: 35580629 ]
- Ntcp (Complex from Mus musculus)
- Complex of ntcp and fab in the presence of myristoylated pres1 peptide (Complex from Homo sapiens)
- Sodium/bile acid cotransporter (38 kDa, Protein from Homo sapiens)
- Fab heavy chain from antibody igg clone number yn69202 (26 kDa, Protein from Mus musculus)
- Fab (Complex)
- Fab light chain from antibody igg clone number yn69202 (24 kDa, Protein from Mus musculus)
Cryo-EM structure of human NTCP (wild-type) complexed with YN69202Fab
Resolution: 3.32 Å
EM Method: Single-particle
Fitted PDBs: 7wsi
Q-score: 0.434
Asami J, Kimura KT, Fujita-Fujiharu Y, Ishida H, Zhang Z, Nomura Y, Liu K, Uemura T, Sato Y, Ono M, Yamamoto M, Noda T, Shigematsu H, Drew D, Iwata S, Shimizu T, Nomura N, Ohto U
Nature (2022) 606 pp. 1021-1026 [ DOI: doi:10.1038/s41586-022-04845-4 Pubmed: 35580629 ]
- Fab light chain from antibody igg clone number yn69202 (24 kDa, Protein from Mus musculus)
- Fab heavy chain from antibody igg clone number yn69202 (26 kDa, Protein from Mus musculus)
- Fab (Complex)
- Complex of ntcp and fab (Complex from Homo sapiens)
- Ntcp (Complex from Homo sapiens)
- Sodium/bile acid cotransporter (38 kDa, Protein from Homo sapiens)