Structure of a human TREX complex (with the THO tetramer).
Structure of an ALYREF-exon junction complex hexamer
Pacheco-Fiallos B, Vorländer MK, Riabov-Bassat D, Fin L, O'Reilly FJ, Ayala FI, Schellhaas U, Rappsilber J, Plaschka C
Nature (2023) 616 pp. 828-835 [ DOI: 10.1038/s41586-023-05904-0 Pubmed: 37020021 ]
Image sets:
- CryoSPARC job of the final 3D reconstruction
- Final particles used for reconstruction
- Motion-corrected and dose-weighted micrographs
- Unaligned multi-frame micrographs of the ALYREF-EJX hexamer in EER format
- Motion-corrected micrographs
- Gainref file for motion correction of compressed movies
Structure of an endogenous human TREX complex bound to mRNA (single particle cryo-EM dataset)
Pacheco-Fiallos B, Vorländer MK, Riabov-Bassat D, Fin L, O'Reilly FJ, Ayala FI, Schellhaas U, Rappsilber J, Plaschka C
Nature (2023) 616 pp. 828-835 [ DOI: 10.1038/s41586-023-05904-0 Pubmed: 37020021 ]
Image sets:
- Motion-corrected micrographs (dataset 1)
- Gainref file for motion correction of compressed tiff movies (dataset 1)
- Gainref file for motion correction of compressed tiff movies (dataset 2)
- Motion-corrected micrographs (dataset 2)
- Unaligned movies in compressed TIFF format collected on a K3 detector (dataset 1)
- Unaligned movies in compressed TIFF format collected on a K3 detector (dataset 2)
Structure of the human TREX core THO-UAP56 complex (map D)
Resolution: 3.45 Å
EM Method: Single-particle
Fitted PDBs: 7znl
Q-score: 0.069
Pacheco-Fiallos B, Vorlander MK, Riabov-Bassat D, Fin L, O'Reilly FJ, Ayala FI, Schellhaas U, Rappsilber J, Plaschka C
Nature (2023) 616 pp. 828-835 [ DOI: doi:10.1038/s41586-023-05904-0 Pubmed: 37020021 ]
- Tho complex subunit 2 (183 kDa, Protein from Homo sapiens)
- Tho complex subunit 1 (75 kDa, Protein from Homo sapiens)
- Spliceosome rna helicase ddx39b (49 kDa, Protein from Homo sapiens)
- Tho complex subunit 5 homolog (78 kDa, Protein from Homo sapiens)
- Tho-uap56 (1 MDa, Complex from Homo sapiens)
- Tho complex subunit 6 homolog (37 kDa, Protein from Homo sapiens)
- Tho complex subunit 3 (38 kDa, Protein from Homo sapiens)
- Tho complex subunit 7 homolog (23 kDa, Protein from Homo sapiens)
Composite map of the human TREX core THO-UAP56 complex
Pacheco-Fiallos B, Vorlander MK, Riabov-Bassat D, Fin L, O'Reilly FJ, Ayala FI, Schellhaas U, Rappsilber J, Plaschka C
Nature (2023) 616 pp. 828-835 [ DOI: doi:10.1038/s41586-023-05904-0 Pubmed: 37020021 ]
- Tho-uap56 (1 MDa, Complex from Homo sapiens)
Cryo electron tomography dataset of purified human TREX-mRNPs
Pacheco-Fiallos B, Vorländer MK, Riabov-Bassat D, Fin L, O'Reilly FJ, Ayala FI, Schellhaas U, Rappsilber J, Plaschka C
Nature (2023) 616 pp. 828-835 [ DOI: 10.1038/s41586-023-05904-0 Pubmed: 37020021 ]
Image sets:
- Motion corrected tilt exposures
- Unbinned, unaligned tilt series as output by SerialEM.
- Extracted subtomograms that were used in the final reconstruction
- Reconstructed tomograms, binned to a pixel size of 10 Å/px
- Compressed movies of individual tilt exposures
- Denoised, reconstructed tomograms, binned to a pixel size of 10 Å/px
Structure of an endogenous human TREX complex bound to mRNA, composite map
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7znk
Q-score: 0.139
Pacheco-Fiallos B, Vorlander MK, Riabov-Bassat D, Fin L, O'Reilly FJ, Ayala FI, Schellhaas U, Rappsilber J, Plaschka C
Nature (2023) 616 pp. 828-835 [ DOI: doi:10.1038/s41586-023-05904-0 Pubmed: 37020021 ]
- Tho complex subunit 2 (183 kDa, Protein from Homo sapiens)
- Tho complex subunit 1 (75 kDa, Protein from Homo sapiens)
- Spliceosome rna helicase ddx39b (49 kDa, Protein from Homo sapiens)
- Tho complex subunit 5 homolog (78 kDa, Protein from Homo sapiens)
- Tho complex subunit 6 homolog (37 kDa, Protein from Homo sapiens)
- Tho complex subunit 3 (38 kDa, Protein from Homo sapiens)
- Tho complex subunit 4 (26 kDa, Protein from Homo sapiens)
- Tho complex subunit 7 homolog (23 kDa, Protein from Homo sapiens)
- Rna (873 Da, RNA from Homo sapiens)
- Endogenous human trex-mrna complex (1 MDa, Complex from Homo sapiens)
Tomogram of purified human TREX-mRNPs
Pacheco-Fiallos B, Vorlander MK, Riabov-Bassat D, Fin L, O'Reilly FJ, Ayala FI, Schellhaas U, Rappsilber J, Plaschka C
Nature (2023) 616 pp. 828-835 [ DOI: doi:10.1038/s41586-023-05904-0 Pubmed: 37020021 ]
- Endogenous human transcription-export complex (1 MDa, Complex from Homo sapiens)
Structure of an endogenous human TREX complex bound to mRNA, map A
Pacheco-Fiallos B, Vorlander MK, Riabov-Bassat D, Fin L, O'Reilly FJ, Ayala FI, Schellhaas U, Rappsilber J, Plaschka C
Nature (2023) 616 pp. 828-835 [ DOI: doi:10.1038/s41586-023-05904-0 Pubmed: 37020021 ]
- Endogenous human trex-mrna complex (1 MDa, Complex from Homo sapiens)
Structure of an endogenous human TREX complex bound to mRNA, map B
Pacheco-Fiallos B, Vorlander MK, Riabov-Bassat D, Fin L, O'Reilly FJ, Ayala FI, Schellhaas U, Rappsilber J, Plaschka C
Nature (2023) 616 pp. 828-835 [ DOI: doi:10.1038/s41586-023-05904-0 Pubmed: 37020021 ]
- Endogenous human trex-mrna complex (1 MDa, Complex from Homo sapiens)