Cryo-EM structure of the IS621 recombinase in complex with bridge RNA, donor DNA, and target DNA
Hiraizumi M, Perry NT, Durrant MG, Soma T, Nagahata N, Okazaki S, Athukoralage JS, Isayama Y, Pai JJ, Pawluk A, Konermann S, Yamashita K, Hsu PD, Nishimasu H
Nature (2024) 630 pp. 994-1002 [ DOI: 10.1038/s41586-024-07570-2 Pubmed: 38926616 ]
Image sets:
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 8wt6
Q-score: 0.604
Hiraizumi M, Perry NT, Durrant MG, Soma T, Nagahata N, Okazaki S, Athukoralage JS, Isayama Y, Pai JJ, Pawluk A, Konermann S, Yamashita K, Hsu PD, Nishimasu H
Nature (2024) 630 pp. 994-1002 [ Pubmed: 38926616 DOI: doi:10.1038/s41586-024-07570-2 ]
- Donor dna (13 kDa, DNA from Escherichia coli)
- Target dna (11 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- The is621 recombinase in complex with bridge rna, donor dna, and target dna in the pre-strand exchange state (Complex from Escherichia coli)
- Donor dna (13 kDa, DNA from Escherichia coli)
- Is621 transposase (36 kDa, Protein from Escherichia coli)
- Bridge rna (57 kDa, RNA from Escherichia coli)
- Target dna (11 kDa, DNA from Escherichia coli)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8wt7
Q-score: 0.563
Hiraizumi M, Perry NT, Durrant MG, Soma T, Nagahata N, Okazaki S, Athukoralage JS, Isayama Y, Pai JJ, Pawluk A, Konermann S, Yamashita K, Hsu PD, Nishimasu H
Nature (2024) 630 pp. 994-1002 [ Pubmed: 38926616 DOI: doi:10.1038/s41586-024-07570-2 ]
- Donor dna (13 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Target dna (11 kDa, DNA from Escherichia coli)
- Bridge rna (57 kDa, RNA from Escherichia coli)
- The is621 recombinase in complex with bridge rna, donor dna, and target dna in the pre-strand exchange locked state (Complex from Escherichia coli)
- Donor dna (13 kDa, DNA from Escherichia coli)
- Is621 transposase (36 kDa, Protein from Escherichia coli)
- Target dna (11 kDa, DNA from Escherichia coli)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8wt8
Q-score: 0.543
Hiraizumi M, Perry NT, Durrant MG, Soma T, Nagahata N, Okazaki S, Athukoralage JS, Isayama Y, Pai JJ, Pawluk A, Konermann S, Yamashita K, Hsu PD, Nishimasu H
Nature (2024) 630 pp. 994-1002 [ Pubmed: 38926616 DOI: doi:10.1038/s41586-024-07570-2 ]
- Donor dna-target dna (10 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Bridge rna (57 kDa, RNA from Escherichia coli)
- Donor dna (13 kDa, DNA from Escherichia coli)
- Is621 transposase (36 kDa, Protein from Escherichia coli)
- The is621 recombinase in complex with bridge rna, donor dna, and target dna in the post-strand exchange state (holliday junction intermediate) (Complex from Escherichia coli)
- Target dna-donor dna (15 kDa, DNA from Escherichia coli)
- Target dna (11 kDa, DNA from Escherichia coli)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8wt9
Q-score: 0.57
Hiraizumi M, Perry NT, Durrant MG, Soma T, Nagahata N, Okazaki S, Athukoralage JS, Isayama Y, Pai JJ, Pawluk A, Konermann S, Yamashita K, Hsu PD, Nishimasu H
Nature (2024) 630 pp. 994-1002 [ Pubmed: 38926616 DOI: doi:10.1038/s41586-024-07570-2 ]
- The is621 recombinase in complex with bridge rna, donor dna, and target dna in the post-strand exchange state (holliday junction resolution) (Complex from Escherichia coli)
- Donor dna-target dna (10 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Bridge rna (57 kDa, RNA from Escherichia coli)
- Donor dna (13 kDa, DNA from Escherichia coli)
- Is621 transposase (36 kDa, Protein from Escherichia coli)
- Target dna-donor dna (15 kDa, DNA from Escherichia coli)
- Target dna (11 kDa, DNA from Escherichia coli)