Cryo-EM Structure of human ABCA7 in BPL/Ch Nanodiscs
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8edw
Q-score: 0.34
Le LTM, Thompson JR, Dehghani-Ghahnaviyeh S, Pant S, Dang PX, French JB, Kanikeyo T, Tajkhorshid E, Alam A
EMBO J (2023) 42 pp. e111065-e111065 [ DOI: doi:10.15252/embj.2022111065 Pubmed: 36484366 ]
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Human abca7 in porcine bpl/ch/msp1d1 nanodiscs (234 kDa, Complex from Homo sapiens)
- Unknown ligand (622 Da, Ligand)
- Phospholipid-transporting atpase abca7 (234 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Human ABCA7 in BPL/Ch nanodiscs (Map 2)
Le LTM, Thompson JR, Dehghani-Ghahnaviyeh S, Pant S, Dang PX, French JB, Kanikeyo T, Tajkhorshid E, Alam A
EMBO J (2023) 42 pp. e111065-e111065 [ DOI: doi:10.15252/embj.2022111065 Pubmed: 36484366 ]
- Human abca7 in porcine bpl/ch/msp1d1 nanodiscs (234 kDa, Complex from Homo sapiens)
Cryo-EM Structure of human ABCA7 in PE/Ch nanodiscs
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 8ee6
Q-score: 0.314
Le LTM, Thompson JR, Dehghani-Ghahnaviyeh S, Pant S, Dang PX, French JB, Kanikeyo T, Tajkhorshid E, Alam A
EMBO J (2023) 42 pp. e111065-e111065 [ DOI: doi:10.15252/embj.2022111065 Pubmed: 36484366 ]
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Human abca7 in porcine bpl/ch/msp1d1 nanodiscs (234 kDa, Complex from Homo sapiens)
- Unknown ligand (622 Da, Ligand)
- Phospholipid-transporting atpase abca7 (234 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Cryo-EM structure of human ABCA7 in Digitonin
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8eeb
Q-score: 0.329
Le LTM, Thompson JR, Dehghani-Ghahnaviyeh S, Pant S, Dang PX, French JB, Kanikeyo T, Tajkhorshid E, Alam A
EMBO J (2023) 42 pp. e111065-e111065 [ DOI: doi:10.15252/embj.2022111065 Pubmed: 36484366 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Human abca7 in digitonin (234 kDa, Complex from Homo sapiens)
- Phospholipid-transporting atpase abca7 (234 kDa, Protein from Homo sapiens)
Cryo-EM Structure of Nanodisc reconstituted human ABCA7 EQ mutant in ATP bound closed state
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8eop
Q-score: 0.407
Le LTM, Thompson JR, Dehghani-Ghahnaviyeh S, Pant S, Dang PX, French JB, Kanikeyo T, Tajkhorshid E, Alam A
EMBO J (2023) 42 pp. e111065-e111065 [ DOI: doi:10.15252/embj.2022111065 Pubmed: 36484366 ]
- Phospholipid-transporting atpase abca7 (234 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Human abca7 eq mutant in porcine bpl/ch/msp1d1 nanodiscs in complex with atp//mg2+ (234 kDa, Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Cryo-EM structure of the GltPh L152C-G321C mutant in the intermediate state.
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6wyj
Q-score: 0.473
Chen I, Pant S, Wu Q, Cater RJ, Sobti M, Vandenberg RJ, Stewart AG, Tajkhorshid E, Font J, Ryan RM
Nature (2021) 591 pp. 327-331 [ Pubmed: 33597752 DOI: doi:10.1038/s41586-021-03240-9 ]
Cryo-EM structure of the GltPh L152C-G321C mutant in the intermediate chloride conducting state.
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6wyk
Q-score: 0.388
Chen I, Pant S, Wu Q, Cater RJ, Sobti M, Vandenberg RJ, Stewart AG, Tajkhorshid E, Font J, Ryan RM
Nature (2021) 591 pp. 327-331 [ Pubmed: 33597752 DOI: doi:10.1038/s41586-021-03240-9 ]
- Aspartic acid (133 Da, Ligand)
- Glutamate transporter homolog gltph monomer in intermediate chloride conducting configuration (Complex from Pyrococcus horikoshii OT3)
- Glutamate transporter homolog (44 kDa, Protein from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3))
Cryo-EM structure of GltPh L152C-G351C mutant in the intermediate outward-facing state.
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6wyl
Q-score: 0.424
Chen I, Pant S, Wu Q, Cater RJ, Sobti M, Vandenberg RJ, Stewart AG, Tajkhorshid E, Font J, Ryan RM
Nature (2021) 591 pp. 327-331 [ Pubmed: 33597752 DOI: doi:10.1038/s41586-021-03240-9 ]