Pre-fusion Measles virus fusion protein complexed with Fab 77
Resolution: 2.56 Å
EM Method: Single-particle
Fitted PDBs: 8ut2
Q-score: 0.583
Zyla DS, Della Marca R, Niemeyer G, Zipursky G, Stearns K, Leedale C, Sobolik EB, Callaway HM, Hariharan C, Peng W, Parekh D, Marcink TC, Diaz Avalos R, Horvat B, Mathieu C, Snijder J, Greninger AL, Hastie KM, Niewiesk S, Moscona A, Porotto M, Ollmann Saphire E
Science (2024) 384 pp. eadm8693-eadm8693 [ DOI: doi:10.1126/science.adm8693 Pubmed: 38935733 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Fusion glycoprotein f0 (44 kDa, Protein from Measles morbillivirus)
- Antibody 77 fab heavy chain, antibody 77 fab light chain (Complex from Mus musculus)
- Fusion glycoprotein f0 (12 kDa, Protein from Measles morbillivirus)
- Mab 77 light chain (25 kDa, Protein from Mus musculus)
- Mab 77 heavy chain (52 kDa, Protein from Mus musculus)
- Measles virus fusion glycoprotein (Complex from Measles morbillivirus)
- Water (18 Da, Ligand)
- Structure of measles fusion glycoprotein in complex with fab 77 (Complex)
Structure of the Measles virus Fusion protein in the post-fusion conformation
Resolution: 2.73 Å
EM Method: Single-particle
Fitted PDBs: 8utf
Q-score: 0.572
Zyla DS, Della Marca R, Niemeyer G, Zipursky G, Stearns K, Leedale C, Sobolik EB, Callaway HM, Hariharan C, Peng W, Parekh D, Marcink TC, Diaz Avalos R, Horvat B, Mathieu C, Snijder J, Greninger AL, Hastie KM, Niewiesk S, Moscona A, Porotto M, Ollmann Saphire E
Science (2024) 384 pp. eadm8693-eadm8693 [ DOI: doi:10.1126/science.adm8693 Pubmed: 38935733 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Fusion glycoprotein f0 (12 kDa, Protein from Measles virus strain Ichinose-B95a)
- Fusion glycoprotein f0 (44 kDa, Protein from Measles virus strain Ichinose-B95a)
- Water (18 Da, Ligand)
- Structure of the measles virus fusion protein in the post-fusion conformation (Complex from Measles virus strain Ichinose-B95a)
Structure of the Measles virus Fusion protein in the pre-fusion conformation
Resolution: 2.11 Å
EM Method: Single-particle
Fitted PDBs: 8uup
Q-score: 0.637
Zyla DS, Della Marca R, Niemeyer G, Zipursky G, Stearns K, Leedale C, Sobolik EB, Callaway HM, Hariharan C, Peng W, Parekh D, Marcink TC, Diaz Avalos R, Horvat B, Mathieu C, Snijder J, Greninger AL, Hastie KM, Niewiesk S, Moscona A, Porotto M, Ollmann Saphire E
Science (2024) 384 pp. eadm8693-eadm8693 [ DOI: doi:10.1126/science.adm8693 Pubmed: 38935733 ]
- Structure of the measles virus fusion protein in the pre-fusion conformation (Complex from Measles virus strain Ichinose-B95a)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Fusion glycoprotein f0 (44 kDa, Protein from Measles virus strain Ichinose-B95a)
- Fusion glycoprotein f0 (12 kDa, Protein from Measles virus strain Ichinose-B95a)
- Water (18 Da, Ligand)
Resolution: 2.34 Å
EM Method: Single-particle
Fitted PDBs: 8uuq
Q-score: 0.663
Zyla DS, Della Marca R, Niemeyer G, Zipursky G, Stearns K, Leedale C, Sobolik EB, Callaway HM, Hariharan C, Peng W, Parekh D, Marcink TC, Diaz Avalos R, Horvat B, Mathieu C, Snijder J, Greninger AL, Hastie KM, Niewiesk S, Moscona A, Porotto M, Ollmann Saphire E
Science (2024) 384 pp. eadm8693-eadm8693 [ DOI: doi:10.1126/science.adm8693 Pubmed: 38935733 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Fusion glycoprotein f0 (12 kDa, Protein from Measles virus strain Ichinose-B95a)
- Structure of the measles virus fusion protein in the pre-fusion conformation with bound [fip-hrc]2-peg11 (Complex from Measles virus strain Ichinose-B95a)
- [fip-hrc]2-peg11 (503 Da, Protein from synthetic construct)
- Fusion glycoprotein f0 (44 kDa, Protein from Measles virus strain Ichinose-B95a)
- Water (18 Da, Ligand)
Fab 77-stabilized MeV F ectodomain fragment
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 9at8
Q-score: 0.444
Zyla DS, Della Marca R, Niemeyer G, Zipursky G, Stearns K, Leedale C, Sobolik EB, Callaway HM, Hariharan C, Peng W, Parekh D, Marcink TC, Diaz Avalos R, Horvat B, Mathieu C, Snijder J, Greninger AL, Hastie KM, Niewiesk S, Moscona A, Porotto M, Ollmann Saphire E
Science (2024) 384 pp. eadm8693-eadm8693 [ DOI: doi:10.1126/science.adm8693 Pubmed: 38935733 ]
- Fusion glycoprotein f0 (57 kDa, Protein from Measles virus strain Ichinose-B95a)
- Mab 77 heavy chain (52 kDa, Protein from Homo sapiens)
- Mab 77 light chain (25 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Measles virus fusion glycoprotein (Complex from Measles virus strain Ichinose-B95a)
- Antibody 77 fab heavy chain, antibody 77 fab light chain (Complex from Mus musculus)
- Fab 77-stabilized mev f ectodomain fragment (43 kDa, Complex)
Structure of EBOV GP lacking the mucin-like domain with 2.1.1D5 scFv and 6D6 scFv bound
Resolution: 2.53 Å
EM Method: Single-particle
Fitted PDBs: 8dpl
Q-score: 0.536
Yu X, Hastie KM, Davis CW, Avalos RD, Williams D, Parekh D, Hui S, Mann C, Hariharan C, Takada A, Ahmed R, Saphire EO
Cell Rep (2023) 42 pp. 113366-113366 [ DOI: doi:10.1016/j.celrep.2023.113366 Pubmed: 37938974 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 2.1.1d5 light chain variable domain (11 kDa, Protein from Homo sapiens)
- 6d6 single-chain variable fragment (25 kDa, Protein from Mus musculus)
- Glycoprotein gp1 (31 kDa, Protein from Ebola virus - Mayinga, Zaire, 1976)
- Structure of ebov gp in complex with 2.1.1d5 scfv and 6d6 scfv (Complex from Ebola virus - Mayinga, Zaire, 1976)
- Glycoprotein gp2 (15 kDa, Protein from Ebola virus - Mayinga, Zaire, 1976)
- 2.1.1d5 heavy chain variable domain (13 kDa, Protein from Homo sapiens)
Structure of EBOV GP lacking the mucin-like domain with 9.20.1A2 Fab and 6D6 scFv bound
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8dpm
Q-score: 0.505
Yu X, Hastie KM, Davis CW, Avalos RD, Williams D, Parekh D, Hui S, Mann C, Hariharan C, Takada A, Ahmed R, Saphire EO
Cell Rep (2023) 42 pp. 113366-113366 [ DOI: doi:10.1016/j.celrep.2023.113366 Pubmed: 37938974 ]
- Antibody 9.20.1a2 fab (Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Antibody 6d6 scfv (25 kDa, Protein from Mus musculus)
- Antibody 9.20.1a2 fab light chain (11 kDa, Protein from Homo sapiens)
- Glycoprotein gp1 (31 kDa, Protein from Ebola virus - Mayinga, Zaire, 1976)
- Complex of ebola virus glycoprotein trimer with 1a2 antibody fab and 6d6 antibody scfv (Complex from Ebola virus - Mayinga, Zaire, 1976)
- Glycoprotein gp2 (15 kDa, Protein from Ebola virus - Mayinga, Zaire, 1976)
- Antibody 9.20.1a2 fab heavy chain (13 kDa, Protein from Homo sapiens)
Native Lassa glycoprotein in complex with neutralizing antibodies 8.9F and 37.2D
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 7uot
Q-score: 0.559
Li H, Buck T, Zandonatti M, Yin J, Moon-Walker A, Fang J, Koval A, Heinrich ML, Rowland MM, Diaz Avalos R, Schendel SL, Parekh D, Zyla D, Enriquez A, Harkins S, Sullivan B, Smith V, Chukwudozie O, Watanabe R, Robinson JE, Garry RF, Branco LM, Hastie KM, Saphire EO
Sci Transl Med (2022) 14 pp. eabq0991-eabq0991 [ DOI: doi:10.1126/scitranslmed.abq0991 Pubmed: 36288283 ]
- Glycoprotein g2 (26 kDa, Protein from Lassa virus)
- 37.2d light chain (variable domain) (11 kDa, Protein from Homo sapiens)
- 37.2d heavy chain (variable domain) (13 kDa, Protein from Homo sapiens)
- Native lassa glycoprotein in complex with 8.9f-scfv and 37.2d-scfv (Complex)
- 8.9f light chain (variable domain) (11 kDa, Protein from Homo sapiens)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus)
- 8.9f heavy chain (variable domain) (15 kDa, Protein from Homo sapiens)
Native Lassa glycoprotein in complex with neutralizing antibodies 12.1F and 37.2D
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 7uov
Q-score: 0.541
Li H, Buck T, Zandonatti M, Yin J, Moon-Walker A, Fang J, Koval A, Heinrich ML, Rowland MM, Diaz Avalos R, Schendel SL, Parekh D, Zyla D, Enriquez A, Harkins S, Sullivan B, Smith V, Chukwudozie O, Watanabe R, Robinson JE, Garry RF, Branco LM, Hastie KM, Saphire EO
Sci Transl Med (2022) 14 pp. eabq0991-eabq0991 [ DOI: doi:10.1126/scitranslmed.abq0991 Pubmed: 36288283 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 12.1f heavy chain (variable domain) (12 kDa, Protein from Homo sapiens)
- Glycoprotein g2 (26 kDa, Protein from Lassa virus)
- 12.1f light chain (variable domain) (11 kDa, Protein from Homo sapiens)
- 37.2d light chain (variable domain) (11 kDa, Protein from Homo sapiens)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus)
- 37.2d heavy chain (variable domain) (13 kDa, Protein from Homo sapiens)
- Native lassa glycoprotein in complex with 12.1f-scfv and 37.2d-scfv (Complex)
Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-80 IgG
Hastie KM, Li H, Bedinger D, Schendel SL, Dennison SM, Li K, Rayaprolu V, Yu X, Mann C, Zandonatti M, Diaz Avalos R, Zyla D, Buck T, Hui S, Shaffer K, Hariharan C, Yin J, Olmedillas E, Enriquez A, Parekh D, Abraha M, Feeney E, Horn GQ, Aldon Y, Ali H, Aracic S, Cobb RR, Federman RS, Fernandez JM, Glanville J, Green R, Grigoryan G, Lujan Hernandez AG, Ho DD, Huang KA, Ingraham J, Jiang W, Kellam P, Kim C, Kim M, Kim HM, Kong C, Krebs SJ, Lan F, Lang G, Lee S, Leung CL, Liu J, Lu Y, MacCamy A, McGuire AT, Palser AL, Rabbitts TH, Rikhtegaran Tehrani Z, Sajadi MM, Sanders RW, Sato AK, Schweizer L, Seo J, Shen B, Snitselaar JL, Stamatatos L, Tan Y, Tomic MT, van Gils MJ, Youssef S, Yu J, Yuan TZ, Zhang Q, Peters B, Tomaras GD, Germann T, Saphire EO
Science (2021) 374 pp. 472-478 [ Pubmed: 34554826 DOI: doi:10.1126/science.abh2315 ]