cAMP-free rabbit HCN4 stabilized in LMNG-CHS detergent mixture
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7np3
Q-score: 0.419
Saponaro A, Bauer D, Giese MH, Swuec P, Porro A, Gasparri F, Sharifzadeh AS, Chaves-Sanjuan A, Alberio L, Parisi G, Cerutti G, Clarke OB, Hamacher K, Colecraft HM, Mancia F, Hendrickson WA, Siegelbaum SA, DiFrancesco D, Bolognesi M, Thiel G, Santoro B, Moroni A
Mol Cell (2021) 81 pp. 2929-2943.e6 [ DOI: doi:10.1016/j.molcel.2021.05.033 Pubmed: 34166608 ]
cAMP-bound rabbit HCN4 stabilized in LMNG-CHS detergent mixture
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7np4
Q-score: 0.451
Saponaro A, Bauer D, Giese MH, Swuec P, Porro A, Gasparri F, Sharifzadeh AS, Chaves-Sanjuan A, Alberio L, Parisi G, Cerutti G, Clarke OB, Hamacher K, Colecraft HM, Mancia F, Hendrickson WA, Siegelbaum SA, DiFrancesco D, Bolognesi M, Thiel G, Santoro B, Moroni A
Mol Cell (2021) 81 pp. 2929-2943.e6 [ DOI: doi:10.1016/j.molcel.2021.05.033 Pubmed: 34166608 ]
Rabbit HCN4 stabilised in amphipol A8-35
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7nmn
Q-score: 0.459
Saponaro A, Bauer D, Giese MH, Swuec P, Porro A, Gasparri F, Sharifzadeh AS, Chaves-Sanjuan A, Alberio L, Parisi G, Cerutti G, Clarke OB, Hamacher K, Colecraft HM, Mancia F, Hendrickson WA, Siegelbaum SA, DiFrancesco D, Bolognesi M, Thiel G, Santoro B, Moroni A
Mol Cell (2021) 81 pp. 2929- [ DOI: doi:10.1016/j.molcel.2021.05.033 Pubmed: 34166608 ]
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 7mjs
Q-score: 0.528
Cater RJ, Chua GL, Erramilli SK, Keener JE, Choy BC, Tokarz P, Chin CF, Quek DQY, Kloss B, Pepe JG, Parisi G, Wong BH, Clarke OB, Marty MT, Kossiakoff AA, Khelashvili G, Silver DL, Mancia F
Nature (2021) 595 pp. 315-319 [ Pubmed: 34135507 DOI: doi:10.1038/s41586-021-03650-9 ]
- Major facilitator superfamily domain containing 2a (60 kDa, Protein from Gallus gallus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 2ag3 fab light chain (23 kDa, Protein from Synthetic construct)
- [(2~{r})-2-oxidanyl-3-[oxidanyl-[2-(trimethyl-$l^{4}-azanyl)ethoxy]phosphoryl]oxy-propyl] (9~{z},12~{z},15~{z})-octadeca-9,12,15-trienoate (518 Da, Ligand)
- Single-particle cryo-em structure of major facilitator superfamily domain containing 2a (mfsd2a) gallus gallus in complex with lpc-18:3 (Complex from Gallus gallus)
- 2ag3 fab heavy chain (25 kDa, Protein from Synthetic construct)
Human WLS in complex with WNT8A
Resolution: 3.19 Å
EM Method: Single-particle
Fitted PDBs: 7kc4
Q-score: 0.506
Nygaard R, Yu J, Kim J, Ross DR, Parisi G, Clarke OB, Virshup DM, Mancia F
Cell (2021) 184 pp. 194- [ Pubmed: 33357447 DOI: doi:10.1016/j.cell.2020.11.038 ]
- Protein wnt-8a (40 kDa, Protein from Homo sapiens)
- Protein wntless homolog (65 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- 1-cis-9-octadecanoyl-2-cis-9-hexadecanoyl phosphatidyl glycerol (746 Da, Ligand)
- Complex of wls/evi and wnt8a in nanodisc (105 kDa, Complex from Homo sapiens)
- Palmitic acid (256 Da, Ligand)