New1 bound to 80S ribosome; A-tRNA, P-tRNA, eIF5a (State 1)
Turnbull K, Paternoga H, von der Weth E, Egorov AA, Pochopien AA, Zhang Y, Nersisyan L, Margus T, Johansson MJO, Pelechano V, Wilson DN, Hauryliuk V
Nucleic Acids Res (2024) 52 pp. 12005-12020 [ Pubmed: 39217469 DOI: doi:10.1093/nar/gkae748 ]
New1 bound to 80S ribosome; A-tRNA, P-E*-tRNA (State 2)
Turnbull K, Paternoga H, von der Weth E, Egorov AA, Pochopien AA, Zhang Y, Nersisyan L, Margus T, Johansson MJO, Pelechano V, Wilson DN, Hauryliuk V
Nucleic Acids Res (2024) 52 pp. 12005-12020 [ Pubmed: 39217469 DOI: doi:10.1093/nar/gkae748 ]
New1 bound to 80S ribosome; A-tRNA, P-tRNA, E-tRNA (State 5)
Turnbull K, Paternoga H, von der Weth E, Egorov AA, Pochopien AA, Zhang Y, Nersisyan L, Margus T, Johansson MJO, Pelechano V, Wilson DN, Hauryliuk V
Nucleic Acids Res (2024) 52 pp. 12005-12020 [ Pubmed: 39217469 DOI: doi:10.1093/nar/gkae748 ]
New1 bound to 80S ribosome; A-tRNA, P-tRNA, E-tRNA (State 6)
Turnbull K, Paternoga H, von der Weth E, Egorov AA, Pochopien AA, Zhang Y, Nersisyan L, Margus T, Johansson MJO, Pelechano V, Wilson DN, Hauryliuk V
Nucleic Acids Res (2024) 52 pp. 12005-12020 [ Pubmed: 39217469 DOI: doi:10.1093/nar/gkae748 ]
New1 bound to 80S ribosome; P-tRNA, E-tRNA (State 4)
Turnbull K, Paternoga H, von der Weth E, Egorov AA, Pochopien AA, Zhang Y, Nersisyan L, Margus T, Johansson MJO, Pelechano V, Wilson DN, Hauryliuk V
Nucleic Acids Res (2024) 52 pp. 12005-12020 [ Pubmed: 39217469 DOI: doi:10.1093/nar/gkae748 ]
New1 bound to 80S ribosome; eRF1, P-tRNA, E-tRNA (State 7)
Turnbull K, Paternoga H, von der Weth E, Egorov AA, Pochopien AA, Zhang Y, Nersisyan L, Margus T, Johansson MJO, Pelechano V, Wilson DN, Hauryliuk V
Nucleic Acids Res (2024) 52 pp. 12005-12020 [ Pubmed: 39217469 DOI: doi:10.1093/nar/gkae748 ]
New1 bound to 80S ribosome; A-P-tRNA, P-E-tRNA (State 3)
Turnbull K, Paternoga H, von der Weth E, Egorov AA, Pochopien AA, Zhang Y, Nersisyan L, Margus T, Johansson MJO, Pelechano V, Wilson DN, Hauryliuk V
Nucleic Acids Res (2024) 52 pp. 12005-12020 [ Pubmed: 39217469 DOI: doi:10.1093/nar/gkae748 ]
PoxtA-EQ2 antibiotic resistance ABCF bound to E. faecalis 70S ribosome, state I
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7p7r
Q-score: -0.017
Crowe-McAuliffe C, Murina V, Turnbull KJ, Huch S, Kasari M, Takada H, Nersisyan L, Sundsfjord A, Hegstad K, Atkinson GC, Pelechano V, Wilson DN, Hauryliuk V
Nat Commun (2022) 13 pp. 1860-1860 [ DOI: doi:10.1038/s41467-022-29274-9 Pubmed: 35387982 ]
- 50s ribosomal protein l34 (5 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- Potassium ion (39 Da, Ligand)
- 50s ribosomal protein l22 (12 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l24 (11 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 70s ribosome (2 MDa, Complex from Enterococcus faecalis)
- 50s ribosomal protein l6 (19 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l18 (12 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s14 type z (7 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s20 (8 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s6 (11 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s7 (17 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- Zinc ion (65 Da, Ligand)
- Spectinomycin (332 Da, Ligand)
- 30s ribosomal protein s4 (23 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l17 (14 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s16 (10 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l13 (16 kDa, Protein from Enterococcus faecalis V583)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 1,4-diaminobutane (88 Da, Ligand)
- 50s ribosomal protein l5 (20 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l16 (16 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- Magnesium ion (24 Da, Ligand)
- Mrna (6 kDa, RNA from Enterococcus faecalis V583)
- 50s ribosomal protein l1 (24 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l23 (11 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- Are-abc-f family resistance factor poxta (67 kDa, Protein from Enterococcus faecalis V583)
- 50s ribosomal protein l20 (13 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s15 (10 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l28 (7 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s9 (14 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l4 (22 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l31 type b (10 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l14 (13 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s12 (15 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l36 (4 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l33 3 (5 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- Complex of poxta-eq2 bound to the 70s ribosome from e. faecalis (initiation complex), state i. (2 MDa, Complex)
- 50s ribosomal protein l21 (11 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l32-3 (6 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s5 (17 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s11 (13 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l29 (7 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l19 (13 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s10 (11 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- Are-abc-f family resistance factor poxta (70 kDa, Complex from Enterococcus faecalis)
- 5s rrna (37 kDa, RNA from Enterococcus faecalis V583)
- 50s ribosomal protein l2 (30 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 23s rrna (944 kDa, RNA from Enterococcus faecalis V583)
- Trna-fmet (24 kDa, RNA from Enterococcus faecalis V583)
- 30s ribosomal protein s8 (14 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l27 (10 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 16s rrna (504 kDa, RNA from Enterococcus faecalis V583)
- 50s ribosomal protein l30 (6 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s2 (29 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s3 (24 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s17 (10 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l3 (22 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s18 (9 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s13 (13 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l35 (7 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 50s ribosomal protein l15 (15 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
- 30s ribosomal protein s19 (10 kDa, Protein from Enterococcus faecalis (strain ATCC 700802 / V583))
E. faecalis 70S ribosome bound by PoxtA-EQ2, high-resolution combined volume
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7p7q
Q-score: 0.572
Crowe-McAuliffe C, Murina V, Turnbull KJ, Huch S, Kasari M, Takada H, Nersisyan L, Sundsfjord A, Hegstad K, Atkinson GC, Pelechano V, Wilson DN, Hauryliuk V
Nat Commun (2022) 13 pp. 1860-1860 [ DOI: doi:10.1038/s41467-022-29274-9 Pubmed: 35387982 ]
- 50s ribosomal protein l29 (7 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l5 (20 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s16 (10 kDa, Protein from Enterococcus faecalis)
- Fmet-trna (24 kDa, RNA from Enterococcus faecalis)
- 50s ribosomal protein l30 (6 kDa, Protein from Enterococcus faecalis)
- Spectinomycin (332 Da, Ligand)
- 50s ribosomal protein l19 (13 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s4 (23 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l31 type b (10 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s8 (14 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l21 (11 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s11 (13 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s20 (8 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l32-3 (6 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s12 (15 kDa, Protein from Enterococcus faecalis)
- Potassium ion (39 Da, Ligand)
- 16s rrna (504 kDa, RNA from Enterococcus faecalis)
- 30s ribosomal protein s9 (14 kDa, Protein from Enterococcus faecalis)
- Complex of poxta-eq2 bound to the 70s ribosome from e. faecalis (initiation complex), state i. (Complex)
- 50s ribosomal protein l1 (24 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l3 (22 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l17 (14 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l22 (12 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s5 (17 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s15 (10 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s2 (29 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l20 (13 kDa, Protein from Enterococcus faecalis)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Are-abc-f family resistance factor poxta (67 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l15 (15 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s19 (10 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l2 (30 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l14 (13 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l18 (12 kDa, Protein from Enterococcus faecalis)
- Water (18 Da, Ligand)
- Poxa (Complex from Enterococcus faecalis)
- 50s ribosomal protein l6 (19 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l34 (5 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l24 (11 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l36 (4 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s18 (9 kDa, Protein from Enterococcus faecalis)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Mrna (6 kDa, RNA from Enterococcus faecalis)
- 50s ribosomal protein l35 (7 kDa, Protein from Enterococcus faecalis)
- 5s rrna (37 kDa, RNA from Enterococcus faecalis)
- 30s ribosomal protein s10 (11 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l13 (16 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l23 (11 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s17 (10 kDa, Protein from Enterococcus faecalis)
- 23s rrna (943 kDa, RNA from Enterococcus faecalis)
- 50s ribosomal protein l33 3 (6 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l16 (16 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s13 (13 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l4 (22 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l28 (7 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s6 (11 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l27 (10 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s7 (17 kDa, Protein from Enterococcus faecalis)
- 70s ribosome (Complex from Enterococcus faecalis)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Enterococcus faecalis)
- 1,4-diaminobutane (88 Da, Ligand)
- 30s ribosomal protein s3 (24 kDa, Protein from Enterococcus faecalis)
PoxtA-EQ2 antibiotic resistance ABCF bound to E. faecalis 70S ribosome, state II
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7p7s
Q-score: 0.526
Crowe-McAuliffe C, Murina V, Turnbull KJ, Huch S, Kasari M, Takada H, Nersisyan L, Sundsfjord A, Hegstad K, Atkinson GC, Pelechano V, Wilson DN, Hauryliuk V
Nat Commun (2022) 13 pp. 1860-1860 [ DOI: doi:10.1038/s41467-022-29274-9 Pubmed: 35387982 ]
- 50s ribosomal protein l29 (7 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l5 (20 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s16 (10 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l30 (6 kDa, Protein from Enterococcus faecalis)
- 70s ribosome (Complex from Enterococcus faecalis)
- Spectinomycin (332 Da, Ligand)
- 50s ribosomal protein l19 (13 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s4 (23 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l31 type b (10 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s8 (14 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l21 (11 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s20 (8 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s12 (15 kDa, Protein from Enterococcus faecalis)
- Potassium ion (39 Da, Ligand)
- 30s ribosomal protein s9 (14 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l1 (24 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l17 (14 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l3 (22 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l16 (16 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l22 (12 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s5 (17 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s15 (10 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s2 (29 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l20 (13 kDa, Protein from Enterococcus faecalis)
- Trna-fmet (25 kDa, RNA from Enterococcus faecalis)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Are-abc-f family resistance factor poxta (67 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l15 (15 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s19 (10 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l2 (30 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l14 (13 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l18 (12 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l6 (19 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l34 (5 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l24 (11 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l36 (4 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s18 (9 kDa, Protein from Enterococcus faecalis)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Mrna (6 kDa, RNA from Enterococcus faecalis)
- Are-abc-f family resistance factor poxta (Complex from Enterococcus faecalis)
- 50s ribosomal protein l35 (7 kDa, Protein from Enterococcus faecalis)
- 23s rrna (944 kDa, RNA from Enterococcus faecalis)
- 5s rrna (37 kDa, RNA from Enterococcus faecalis)
- 30s ribosomal protein s10 (11 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l13 (16 kDa, Protein from Enterococcus faecalis)
- Complex of poxta-eq2 bound to the 70s ribosome from e. faecalis (initiation complex), state ii. (Complex)
- 50s ribosomal protein l23 (11 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s17 (10 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s13 (13 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l4 (22 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l28 (7 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s11 (13 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s6 (11 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l27 (10 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l32 (6 kDa, Protein from Enterococcus faecalis)
- 50s ribosomal protein l33 (6 kDa, Protein from Enterococcus faecalis)
- 16s rrna (504 kDa, RNA from Enterococcus faecalis)
- 30s ribosomal protein s7 (17 kDa, Protein from Enterococcus faecalis)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Enterococcus faecalis)
- 1,4-diaminobutane (88 Da, Ligand)
- 30s ribosomal protein s3 (24 kDa, Protein from Enterococcus faecalis)